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The impact of freezing and toasting on the glycaemic response of white bread

Abstract

Objective:

To investigate the impact of freezing and toasting on the glycaemic response of white bread.

Subjects/methods:

Ten healthy subjects (three male, seven female), aged 22–59 years, recruited from Oxford Brookes University and the local community. A homemade white bread and a commercial white bread were administered following four different storage and preparation conditions: (1) fresh; (2) frozen and defrosted; (3) toasted; (4) toasted following freezing and defrosting. They were administered randomized repeated measures design. Incremental blood glucose, peak glucose response, 2 h incremental area under the glucose response curve (IAUC).

Results:

The different storage and preparation conditions resulted in lower blood glucose IAUC values compared to both types of fresh white bread. In particular, compared to the fresh homemade bread (IAUC 259 mmol min/l), IAUC was significantly lower when the bread was frozen and defrosted (179 mmol min/l, P<0.05), toasted (193 mmol min/l, P<0.01) and toasted following freezing and defrosting (157 mmol min/l, P<0.01). Similarly, compared to the fresh commercial white bread (253 mmol min/l), IAUC was significantly lower when the bread was toasted (183 mmol min/l, P<0.01) and frozen, defrosted and toasted (187 mmol min/l, P<0.01).

Conclusions:

All three procedures investigated, freezing and defrosting, toasting from fresh and toasting following freezing and defrosting, favourably altered the glucose response of the breads. This is the first study known to the authors to show reductions in glycaemic response as a result of changes in storage conditions and the preparation of white bread before consumption. In addition, the study highlights a need to define and maintain storage conditions of white bread if used as a reference food in the determination of the glycaemic index of foods.

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References

  • Baik M-Y, Dickinson LC, Chinachoti P (2003). Solid-state 13C CP/MAS NMR studies on aging of starch in white bread. J Agric Food Chem 51, 1242–1248.

    CAS  Article  PubMed  Google Scholar 

  • Belderok B (2000). Developments in bread-making processes. Plant Foods Hum Nutr 55, 1–86.

    CAS  Article  PubMed  Google Scholar 

  • Berry CS (1986). Resistant starch: formation and measurement of starch that survives exhaustive digestion with amylolytic enzymes during the fermentation of dietary fibre. J Cereal Sci 4, 301–314.

    CAS  Article  Google Scholar 

  • Bjorck I, Granfeldt Y, Liljeberg H, Tovar J, Asp NG (1994). Food properties affecting the digestion and absorption of carbohydrates. Am J Clin Nutr 59, S699–S705.

    Article  Google Scholar 

  • Bland JM, Altman DG (1986). Statistical methods for assessing agreement between two methods of clinical measurement. Lancet I, 307–310.

    Article  Google Scholar 

  • Brand JC, Nicholson PL, Thorburn AW, Truswell AS (1985). Food processing and the glycemic index. Am J Clin Nutr 42, 1192–1196.

    CAS  Article  PubMed  Google Scholar 

  • Brand-Miller JC, Hayne S, Petocz P, Colagiuri S (2003). Low-glycemic index diets in the management of diabetes. A meta-analysis of randomized controlled trials. Diabetes Care 26, 2261–2267.

    Article  PubMed  Google Scholar 

  • Brouns F, Bjorck I, Frayn KN, Gibbs AL, Lang V, Slama G et al. (2005). Glycaemic index methodology. Br J Nutr 18, 145–171.

    CAS  Google Scholar 

  • Department for Environment, Food and Rural Affairs (2006). Family Food in 2004-05. TSO: London.

  • Englyst HN, Kingman SM, Cummings JH (1992). Classification and measurement of nutritionally important starch fractions. Eur J Clin Nutr 46, S33–S50.

    PubMed  Google Scholar 

  • FAO/WHO (1998). Carbohydrates in Human Nutrition Report of a Joint FAO/WHO Expert Consultation. FAO: Rome.

  • Federation of Bakers (2005). The British Bread and Bakery Snacks Market Factsheet No. http://www.bakersfederation.org.uk/publications/FS3%20-%20UK%20Bakery%20Market.pdf.

  • Frei M, Siddhuraju P, Becker K (2003). Studies on the in vitro starch digestibility and the glycaemic index of six different indigenous rice cultivars from the Philippines. Food Chem 83, 395–402.

    CAS  Article  Google Scholar 

  • Frost G, Leeds A, Trew G, Margara R, Dornhorst A (1998). Insulin sensitivity in women at risk of coronary heart disease and the effect of a low glycemic diet. Metabolism 47, 1245–1251.

    CAS  Article  PubMed  Google Scholar 

  • Frost G, Leeds AA, Dore CJ, Madeiros S, Brading S, Dornhorst A (1999). Glycaemic index as a determinant of serum HDL-cholesterol concentration. Lancet 353, 1045–1048.

    CAS  Article  PubMed  Google Scholar 

  • Goesaert H, Brijs K, Veraverbeke WS, Courtin CM, Gebruers K, Delcour JA (2005). Wheat flour constituents: how they impact bread quality, and how they impact their functionality. Trends Food Sci Tech 16, 12–30.

    CAS  Article  Google Scholar 

  • Granfeldt Y, Eliasson A-C, Bjork I (2000). An examination of the possibility of lowering the glycaemic index of oat and barley flakes by minimal processing. J Nutr 130, 2207–2214.

    CAS  Article  PubMed  Google Scholar 

  • Gray JA, Bemiller JN (2003). Bread staling: molecular basis and control. Comp Rev Food Sci Food Safety 2, 1–21.

    CAS  Article  Google Scholar 

  • Higgins JA, Higbee DR, Donahoo WT, Brown IL, Bell ML, Bessesen DH (2004). Resistant starch consumption promote lipid oxidation. Nutr Metab 1, 8.

    Article  Google Scholar 

  • Hoebler C, Karinthi A, Chiron H, Champ M, Barry JL (1999). Bioavailability of starch in bread rich in amylose: metabolic responses in healthy subjects and starch structure. Eur J Clin Nutr 53, 360–366.

    CAS  Article  PubMed  Google Scholar 

  • Hoebler C, Karinthi A, Devaux MF, Guillon F, Gallant DJG, Bouchet B et al. (1998). Physical and chemical transformations of cereal food during oral digestion in human subjects. Br J Nutr 80, 429–436.

    CAS  Article  PubMed  Google Scholar 

  • Jenkins DJA, Wolever TMS, Taylor RH, Barker H, Fielden H, Baldwin JM et al. (1981). Glycemic index of foods: a physiological basis for carbohydrate exchange. Am J Clin Nutr 34, 362–366.

    CAS  Article  PubMed  Google Scholar 

  • Lang V, Vitapole D (2004). Development of a range of industrialised cereal-based foodstuffs, high in slowly digestible starch. In: A-C Elliasson (ed). Starch in Food: Structure, Function and Applications. Woodhead Publishing: Cambridge. pp. 477–504.

    Chapter  Google Scholar 

  • Larsen HN, Rasmussen OW, Rasmussen PH, Alstrup KK, Biswas SK, Tetens I et al. (2002). Glycaemic index of parboiled rice depends on the severity of processing: study in type 2 diabetic subjects. Eur J Clin Nutr 54, 380–385.

    Article  Google Scholar 

  • Ludwig DS (2000). The glycemic index. Physiological mechanisms relating to obesity, diabetes, and cardiovascular disease. JAMA 287, 2414–2423.

    Article  Google Scholar 

  • Macintosh CG, Holt SHA, Brand-Miller JC (2003). The degree of fat saturation does not alter glycaemic, insulinaemic or satiety responses to a starchy staple in healthy men. J Nutr 133, 2577–2580.

    CAS  Article  PubMed  Google Scholar 

  • McCleary BV, Monaghan DA (2002). Measurement of resistant starch. J AOAC Int 85, 665–675.

    CAS  PubMed  Google Scholar 

  • Robertson MD, Currie JM, Morgan LM, Jewell DP, Frayn KN (2003). Prior short-term consumption of resistant starch enhances postprandial insulin sensitivity in healthy subjects. Diabetologia 46, 659–665.

    CAS  Article  PubMed  Google Scholar 

  • Salmeron J, Ascherio A, Rimm EB, Colditz GA, Spiegelman D, Jenkins DJ et al. (1997a). Dietary fiber, glycaemic load, and risk of NIDDM in men. Diabetes Care 20, 545–550.

    CAS  Article  PubMed  Google Scholar 

  • Salmeron J, Manson JE, Stampfer MJ, Colditz GA, Wing AL, Willet EC (1997b). Dietary fiber, glycaemic load, and risk of non-insulin-dependent diabetes mellitus in women. JAMA 277, 472–477.

    CAS  Article  PubMed  Google Scholar 

  • Warren JM, Henry CJK, Simonite V (2003). Low glycaemic index breakfasts and reduced food intake in preadolescent children. Pediatrics 112, E414–E419.

    Article  PubMed  Google Scholar 

  • Wolever TMS (1990). The glycemic index. World Rev Nutr Diet 62, 120–185.

    CAS  Article  PubMed  Google Scholar 

  • Wolever TMS (2006). The Glycaemic Index. A Physiological Classification of Dietary Carbohydrate. Wallingford: CABI.

    Book  Google Scholar 

  • Wolever TMS, Mehling CC (2003). Long-term effect of varying the source or amount of dietary CHO on postprandial plasma glucose, insulin, triacylglycerol, and free fatty acid concentrations in subjects with impaired glucose tolerance. Am J Clin Nutr 77, 612–621.

    CAS  Article  PubMed  Google Scholar 

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Acknowledgements

Sponsorship: This study was supported by a Biotechnology and Biological Sciences Research Council studentship.

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Correspondence to H J Lightowler.

Additional information

Contributors: PB planned and designed the study and was responsible for the collection of data and writing the manuscript. HJL advised on the study design and contributed to the preparation of the manuscript.

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Burton, P., Lightowler, H. The impact of freezing and toasting on the glycaemic response of white bread. Eur J Clin Nutr 62, 594–599 (2008). https://doi.org/10.1038/sj.ejcn.1602746

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  • DOI: https://doi.org/10.1038/sj.ejcn.1602746

Keywords

  • glycaemic response
  • bread
  • food storage
  • food preparation
  • blood glucose

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