Abstract
Objective:
To assess the effects of plant sterol or stanol ester consumption on their incorporation into erythrocytes and their effects on osmotic fragility of red blood cells.
Design:
Double-blind, randomized, placebo-controlled intervention trial.
Subjects and intervention:
Forty-one subjects on stable statin treatment – who already have increased serum plant sterol and stanol concentrations – first received for 4 weeks a control margarine. For the next 16 weeks, subjects were randomly assigned to one of three possible interventions. Eleven subjects continued with control margarine, 15 subjects with plant sterol ester enriched and 15 subjects with plant stanol ester-enriched margarine. Daily plant sterol or stanol intake was 2.5 g. Erythrocyte haemolysis was measured spectrophotometrically at five different saline concentrations.
Results:
Despite significant (P=0.004) increases of, respectively, 42 and 59% in cholesterol-standardized serum sitosterol and campesterol concentrations in the plant sterol group as compared to the control group, campesterol levels in the red blood cells did not change (P=0.196). Osmotic fragility did not change significantly (P=0.757) in the plant sterol and plant stanol groups as compared to the control group.
Conclusion:
We conclude that plant sterol and stanol ester consumption for 16 weeks does not change osmotic fragility of erythrocytes in statin-treated patients.
Sponsorship:
Netherlands Organisation for Health Research and Development (Program Nutrition: Health, Safety and Sustainability, Grant 014-12-010)
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References
Bjorkhem I, Boberg KM (1994). Inborn errors in bile acid biosynthesis and storage of sterols other than cholesterol. In: Sciver CR, Beaudet AL, Sly WS, Valee D (eds). The Metabolic Base of Inherited Diseases. McGraw Hill: New York, pp. 2073–2100.
Bruckerdorfer KR, Demel RA, de Gier J, van Deenen LLM (1969). The effect of partial replacement of membrane cholesterol by other steroids on the osmotic fragility and glycerol permeability of erythrocytes. Biochim Biophys Acta 183, 334–345.
Candan F, Gultekin F (2002). Effect of vitamin C and zinc on osmotic fragility and lipid peroxidation in zinc-deficient haemodialysis patients. Cell Biochem Funct 20, 95–98.
Chen J, Batta A, Zheng S, Fitzgibbon WR, Ullian ME, Yu H et al. (2005). The missense mutation in Abcg5 gene in spontaneously hypertensive rats (SHR) segregates with phytosterolemia but not hypertension. BMC Genet 6, 40.
de Jong A, Plat J, Mensink RP (2003). Metabolic effects of plant sterols and stanols [review]. J Nutr Biochem 14, 362–369.
Ebine N, Jia X, Demonty I, Wang Y, Jones PJ (2005). Effects of a water-soluble phytostanol ester on plasma cholesterol levels and red blood cell fragility in hamsters. Lipids 40, 175–180.
Godal HC, Elde AT, Nyborg N, Brosstad F (1980). The normal range of osmotic fragility of red blood cells. Scand J Haematol 25, 107–112.
Hendriks HF, Brink EJ, Meijer GW, Princen HM, Ntanios FY (2003). Safety of long-term consumption of plant sterol esters-enriched spread. Eur J Clin Nutr 57, 681–692.
Jenkins DJ, Kendall CW, Faulkner D, Vidgen E, Trautwein EA, Parker TL et al. (2002). A dietary portfolio approach to cholesterol reduction: combined effects of plant sterols, vegetable proteins, and viscous fibers in hypercholesterolemia. Metabolism 51, 1596–1604.
Katan MB, Grundy SM, Jones P, Law M, Miettinen T, Paoletti R (2003). Efficacy and safety of plant stanols and sterols in the management of blood cholesterol levels. Mayo Clin Proc 78, 965–978.
Ketomaki AM, Gylling H, Antikainen M, Siimes MA, Miettinen TA (2003). Red cell and plasma plant sterols are related during consumption of plant stanol and sterol ester spreads in children with hypercholesterolemia. J Pediatr 142, 524–531.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951). Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265–275.
Lu K, Lee M, Hazard S, Brooks-Wilson A, Hidaka H, Kojima H et al. (2001). Two genes that map to the STSL locus cause sitosterolemia: genomic structure and spectrum of mutations involving sterolin-1 and sterolin-2 encoded by ABCG5 and ABCG8, respectively. Am J Hum Gen 69, 278–290.
Miettinen TA, Gylling H, Lindbohm N, Miettinen TE, Rajaratnam RA, Relas H (2003). Serum noncholesterol sterols during inhibition of cholesterol synthesis by statins. J Lab Clin Med 141, 131–137.
Moghadasian MH, Nguyen LB, Shefer S, McManus BM, Frohlich JJ (1999). Histologic, hematologic, and biochemical characteristics of apo E-deficient mice: effects of dietary cholesterol and phytosterols. Lab Invest 79, 355–364.
Naito Y, Konishi C, Ohara N (2000). Blood coagulation and osmolar tolerance of erythrocytes in stroke-prone spontaneously hypertensive rats given rapeseed oil or soybean oil as the only dietary fat. Toxicol Lett 116, 209–215.
Naito Y, Nagata T, Takano Y, Nagatsu T, Ohara N (2003). Rapeseed oil ingestion and exacerbation of hypertension-related conditions in stroke prone spontaneously hypertensive rats. Toxicology 187, 205–216.
Ogawa H, Yamamoto K, Kamisako T, Meguro T (2003). Phytosterol additives increase blood pressure and promote stroke onset in salt-loaded stroke-prone spontaneously hypertensive rats. Clin Exp Pharmacol Physiol 30, 919–924.
Ostlund Jr RE, McGill JB, Zeng CM, Covey DF, Stearns J, Stenson WF et al. (2002). Gastrointestinal absorption and plasma kinetics of soy Delta(5)- phytosterols and phytostanols in humans. Am J Physiol Endocrinol Metab 282, E911–916.
Plat J, Mensink RP (2000). Vegetable oil based versus wood based stanol ester mixtures: effects on serum lipids and hemostatic factors in non-hypercholesterolemic subjects. Atherosclerosis 148, 101–112.
Plat J, Mensink RP (2001). Effects of diets enriched with two different plant sterol ester mixtures on plasma ubiquinol-10 and fat-soluble antioxidant concentrations. Metabolism 50, 520–529.
Ratnayake WM, Plouffe L, L'Abbe MR, Trick K, Mueller R, Hayward S (2003). Comparative health effects of margarines fortified with plant sterols and stanols on a rat model for hemorrhagic stroke. Lipids 38, 1237–1247.
Ratnayake WMN, L'Abbe MR, Mueller R, Hayward S, Plouffe L, Hollywood R et al. (2000). Vegetable oils high in phytosterols make erythrocytes less deformable and shorten the life span of stroke-prone spontaneously hypertensive rats. J Nutr 130, 1166–1178.
Sanders DJ, Minter HJ, Howes D, Hepburn PA (2000). The safety evaluation of phytosterol esters. Part 6. The comparative absorption and tissue distribution of phytosterols in the rat. Food Chem Toxicol 38, 485–491.
Scoggan KA, Gruber H, Lariviere K (2003). A missense mutation in the rat Abcg5 gene causes phytosterolemia in stroke-prone spontaneously hypertensive (SHRSP), spontaneously hypertensive (SHR), and normotensive (WKY inbred) rats. J Lipid Res 44, 911–916.
Srour MA, Bilto YY, Juma M, Irhimeh MR (2000). Exposure of human erythrocytes to oxygen radicals causes loss of deformability, increased osmotic fragility, lipid peroxidation and protein degradation. Clin Hemorheol Microcirc 23, 13–21.
Suhail M, Rizvi SI (1987). Red cell membrane (Na+/K+)-ATPase in diabetes mellitus. Biochem Biophys Res Commun 146, 179–186.
Tatematsu K, Fuma SY, Nagase T, Ichikawa Y, Fujii Y, Okuyama H (2004). Factors other than phytosterols in some vegetable oils affect the survival of SHRSP rats. Food Chem Toxicol 42, 1443–1451.
Acknowledgements
We thank Mirjam Heinen for dietary support and Frank Cox and Ilona Beugels for technical support. We are grateful to Raisio Group, Raisio, Finland for providing us with all the margarines.
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Guarantor: RP Mensink.
Contributors: AJ conducted the study, performed the osmotic fragility measurements and statistically analysed the data. RPM and JP designed and supervised the study. All authors contributed to the writing of the paper.
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de Jong, A., Plat, J. & Mensink, R. Plant sterol or stanol consumption does not affect erythrocyte osmotic fragility in patients on statin treatment. Eur J Clin Nutr 60, 985–990 (2006). https://doi.org/10.1038/sj.ejcn.1602409
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DOI: https://doi.org/10.1038/sj.ejcn.1602409
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