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Factors influencing blood concentration of retinol, α-tocopherol, vitamin C, and β-carotene in the French participants of the SU.VI.MAX trial

European Journal of Clinical Nutrition volume 60, pages 706–717 (2006)Cite this article

Abstract

Objectives:

The data was collected during the inclusion step of the SUpplémentation en VItamines et Minéraux AntioXydants (SU.VI.MAX) study. This article deals with the study's first stage before any supplementation. The collected data shows factors influencing blood vitamin concentrations and may reflect the vitamin status of volunteers.

Material and methods:

A total of 12 741 volunteers were enrolled in the SU.VI.MAX study 7713 women 35–60 years of age and 5028 men 50–60 years of age.

The serum concentrations of retinol, α-tocopherol, and β-carotene were measured by HPLC, and vitamin C concentration was measured by spectrofluorimetry using a Technicon continuous flow analysis apparatus.

The volunteers recorded their 24 h diet by means of a specific terminal that was connected to the main central computer of the SU.VI.MAX study. Volunteers recorded the food they consumed daily and estimated its quantity by comparing pictures of dishes.

Results:

Retinol concentration was significantly higher in older volunteers, and was higher in male than in female volunteers. Smoking had no effect on serum retinol, but the latter was higher in the autumn than in the winter. Serum retinol concentrations were higher in the Southwest region and lower in the Ile-de-France and East-Centre regions. Serum α-tocopherol was slightly higher in older volunteers and also higher in male volunteers. Serum α-tocopherol was significantly lower in smokers, and former smokers showed intermediate levels. Like retinol, serum α-tocopherol was higher in the autumn, and higher in the Southwest as compared to the East-Centre Serum β-carotene was slightly higher in younger volunteers, and concentrations were higher in female than in male volunteers. Tobacco smoking decreased serum β-carotene, which was higher in the autumn, and higher in the East, West, and North regions. Serum vitamin C was higher in female volunteers, and was not age related. Serum vitamin C was lower in smokers, was season-dependant, but contrary to fat-soluble vitamins, concentrations were higher in the winter and spring. Serum vitamin C was higher in the Southeast and East-Centre, but lower in the North region.

Conclusion:

These results suggest that serum retinol concentrations depend on gender, age, seasons, and location of residence. Similarly, serum α-tocopherol concentrations were slightly influenced by age, but more by tobacco smoking, seasons, dietary intake, and location of residence. Serum concentrations of β-carotene depend on gender, age, smoking status, dietary intake, and location of residence. Serum vitamin C concentrations depend on gender, age, smoking status, seasons, dietary intake, and location of residence. Contrary to β-carotene, retinol concentrations were higher in male than in female volunteers. Such a reversed relation suggests a higher β-carotene-retinol conversion in male volunteers.

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References

  • Arnaud J, Fortis I, Blachier S, Kia D, Favier A (1991). Simultaneous determination of retinol, alpha-tocopherol and beta-carotene in serum by isocratic high-performance liquid chromatography. J Chromatogr 572, 103–116.

    Article  CAS  Google Scholar 

  • Baeten JM, Richardson BA, Bankson DD, Wener MH, Kreiss JK, Lavreys L et al. (2004). Use of serum retinol-binding protein for prediction of retinol deficiency: effects of HIV-1 infection, protein malnutrition, and the acute phase response. Am J Clin Nutr 79, 218–225.

    Article  CAS  Google Scholar 

  • Ballew C, Bowman BA, Sowell AL, Gillespie C (2001). Serum retinol distributions in residents of the United States: third National Health and Nutrition Examination Survey, 1988–1994. Am J Clin Nutr 73, 586–593.

    Article  CAS  Google Scholar 

  • Baudier F, Rotily M, Le Bihan G, Janvrin MP, Michaud C (1996). A Survey on Knowledge, Attitudes and Eating Behaviours of the French Between 18 and 75 Years old. Collection ‘Baromètres’ INPES: Paris, France.

    Google Scholar 

  • Bender DA (2003). Nutritional Biochemistry of the Vitamins. Cambridge University Press: Cambridge.

    Book  Google Scholar 

  • Benzie IFF (1999). Vitamin C: prospective functional markers for defining optimal nutritional status. Proc Nutr Soc 58/2, 469–476.

    Article  Google Scholar 

  • Block G, Dresser C, Hartman AM, Caroll DM (1985). Nutrient sources in the American diet: quantitative data from the NHANES II Survey I. Vitamins and minerals. Am J Epidemiol 122, 13–26.

    Article  CAS  Google Scholar 

  • Blomhoff R (1994). Vitamin A in Health and Disease. Marcel Dekker: New York.

    Book  Google Scholar 

  • Bourgeois CF, Chartois HR, Coustans MF, George PR (1989). Dosage automatisé de la vitamine C dans les aliments et les milieux biologiques. Analusis 17/9, 519–525.

    Google Scholar 

  • de Carvalho MJ, Guilland JC, Moreau D, Boggio V, Fuchs F (1996). Vitamin status of healthy volunteers in Burgundy (France). Ann Nutr Metab 40, 24–51.

    Article  CAS  Google Scholar 

  • de Pee S, Dary O (2002). Biochemical indicators of retinol deficiency: serum retinol and serum retinol binding protein. J Nutr 132, 2895S–2901S.

    Article  CAS  Google Scholar 

  • Gibson RS (1990). Principles of Nutritional Assessment. Oxford University Press: New York, Oxford.

    Google Scholar 

  • Girodon F, Galan P, Monget A-L, Boutron-Ruault M-C, Brunet-Lecomte P, Preziosi P et al. (1999). Impact of trace elements and vitamin supplementation on immunity and infections in institutionalized elderly patients. Arch Intern Med 159, 748–754.

    Article  CAS  Google Scholar 

  • Gross M, Yu X, Hannan P, Prouty C, Jacobs DR (2003). Lipid standardization of serum fat-soluble antioxidant concentrations: the YALTA study. Am J Clin Nutr 77, 458–466.

    Article  CAS  Google Scholar 

  • Handelman GL, Packer L, Cross C (1996). Destruction of tocopherols, carotenoids, and retinol in human plasma by cigarette smoke. Am J Clin Nutr 63, 559–565.

    Article  CAS  Google Scholar 

  • Hercberg S, Preziosi P, Galan P, Devanlay M, Keller H, Bourgeois C et al. (1994). Vitamin status of a healthy French population: dietary intakes and biochemical markers. Int J Vitam Nutr Res 64, 220–232.

    CAS  PubMed  Google Scholar 

  • Hercberg S, Preziosi P, Briancon S, Galan P, Triol I, Malvy D et al. (1998a). A primary prevention trial using nutritional doses of antioxidant vitamins and minerals in cardiovascular diseases and cancers in a general population: the SU.VI.MAX study–design, methods, and participant characteristics. Supplementation en VItamines et Mineraux AntioXydants. Control Clin Trials 19, 336–351.

    Article  CAS  Google Scholar 

  • Hercberg S, Galan P, Preziosi P, Roussel AM, Arnaud J, Richard MJ et al. (1998b). Background and rationale behind the SU.VI.MAX study, a prevention trial using nutritional doses of a combination of antioxidant vitamins and minerals to reduce cardivascular and cancer diseases. Int J Vitam Nutr Res 68, 3–20.

    CAS  PubMed  Google Scholar 

  • Hercberg S, Galan P, Preziosi P, Bertrais S, Mennen L, Malvy D et al. (2004). The SU.VI.MAX Study: a randomized, placebo-controlled trial of the health effects of antioxidant vitamins and minerals. Arch Intern Med 164, 2335–2342.

    Article  CAS  Google Scholar 

  • Hercberg S, Preziosi P, Galan P, Faure H, Arnaud J, Duport N et al. (1999). ‘The SU.VI.MAX study’: a primary prevention trial using nutritional doses of antioxidant vitamins and minerals in cardiovascular diseases and cancers. Food Chem Res Toxicol 37, 925–930.

    Article  CAS  Google Scholar 

  • Jordan P, Brubacher D, Moser U, Stahelin HB, Gey KF (1995). α-tocopherol and retinol concentrations in plasma adjusted for cholesterol and triglycerides by multiple regression. Clin Chem 41, 924–927.

    CAS  PubMed  Google Scholar 

  • Krasinski SD, Russell RM, Otradovec CL, Sadowski JA, Hartz SC, Jacob RA et al. (1989). Relationship of retinol and α-tocopherol intake to fasting plasma retinol, retinol-binding protein, retinyl esters, carotene, alpha-tocopherol, and cholesterol among elderly people and young adults: increased plasma retinyl esters among retinol-supplement users. Am J Clin Nutr 49, 112–120.

    Article  CAS  Google Scholar 

  • Lachili B, Faure H, Arnaud J, Richard MJ, Benlatreche C, Favier A et al. (2001). Roussel AM. Blood micornutrients in Algeria, relationships with sex and age. Int J Vitam Nutr Res 71, 111–116.

    Article  CAS  Google Scholar 

  • Lehmann J, Rao DD, Canary JJ, Judd JT (1988). α-tocopherol and relationships among tocopherols in human plasma, platelets, lymphocytes, and red blood cells. Am J Clin Nutr 47, 470–474.

    Article  CAS  Google Scholar 

  • Le Moullec N, Deheeger M, Preziosi P, Montero P, Valeix P, Rolland-Cachera M-F et al. (1996). Validation du manuel-photos utilise' pour l'enquête alimentaire de l'étude SUVIMAX. Can Nutr Diet 31, 158–164.

    Google Scholar 

  • Marangon K, Herbeth B, Lecomte E, Paul-Dauphin A, Grolier P, Chancerelle Y et al. (1998). Diet, antioxidant status, and smoking habits in French men. Am J Clin Nutr 67, 231–239.

    Article  CAS  Google Scholar 

  • Margetts BM, Jackson AA (1996). The determinants of plasma beta-carotene: interaction between smoking and other lifestyle factors. Eur J Clin Nutr 50, 236–238.

    CAS  PubMed  Google Scholar 

  • Margolis SA, Duewer DL (1996). Measurement of ascorbic acid in human plasma and serum: stability, intralaboratory repeatability, and interlaboratory reproducibility. Clin Chem 42, 1257–1262.

    CAS  PubMed  Google Scholar 

  • Ness AR, Khaw KT, Bingham S, Day NE (1996). Vitamin C status and serum lipids. Eur J Clin Nutr 50, 724–729.

    CAS  PubMed  Google Scholar 

  • Nyssonen K, Parviainen MT, Salonen R, Tuomilehto J, Salonen JT (1997). Vitamin C deficiency and risk of myocardial infarction: prospective population study of men from eastern Finland. BMJ 314, 634–638.

    Article  Google Scholar 

  • Olmedilla B, Granado F, Blanco I, Rojas-Hidalgo E (1994). Seasonal and sex-related variations in six serum carotenoids, retinol, and alpha-tocopherol. Am J Clin Nutr 60, 106–110.

    Article  CAS  Google Scholar 

  • Olmedilla B, Granado F, Southon S, Wright AJ, Blanco I, Gil-Martinez E et al. (2001). Serum concentrations of carotenoids and vitamins A, E, and C in control volunteers from five European countries. Br J Nutr 85, 227–238.

    Article  CAS  Google Scholar 

  • Pamuk ER, Byers T, Coates RJ, Vann JW, Sowell AL, Gunter EW et al. (1994). Effect of smoking on serum nutrient concentrations in African-American women. Am J Clin Nutr 59, 891–895.

    Article  CAS  Google Scholar 

  • Papas A, Stacewicz-Sapuntzakis M, Lagiou P, Bamia C, Chlopstios Y, Trichopoulos A (2003). Plasma retinol and tocopherol levels in relation to demographics, lifestyle and nutritional factors of plant origin in Greece. Br J Nutr 89/1, 83–87.

    Article  Google Scholar 

  • Parlesak A, Menzl I, Feuchter A, Bode JC, Bode C (2000). Inhibition of retinol oxidation by ethanol in the rat liver and colon. Gut 47, 825–831.

    Article  CAS  Google Scholar 

  • Rautalahti M, Albanes D, Haukka J, Roos E, Gref C-G, Virtamo J (1993). Seasonal variation of serum concentrations of beta-carotene and alpha-tocopherol. Am J Clin Nutr 57, 551–556.

    Article  CAS  Google Scholar 

  • Russel RM (2000). The retinol spectrum: from deficiency to toxicity. Am J Clin Nutr 71, 878–884.

    Article  Google Scholar 

  • Saintot M, Astre C, Scali J, Gerber M (1995). Within-volunteers seasonal variation and determinants of inter-individual variations of plasma beta-carotene. Int J Vitam Nutr Res 65, 169–174.

    CAS  PubMed  Google Scholar 

  • Sauberlich HE (1975). Human requirements and needs. Vitamin C status: methods and findings. Ann NY Acad Sci 258, 438–450.

    Article  CAS  Google Scholar 

  • Sauberlich HE (1999). Laboratory Tests for the Assessment of Nutritional Status. CRC Press Boca Raton: London, New York, Washington, DC.

    Google Scholar 

  • Scott KJ, Thurnham DI, Hart DJ, Bingham SA, Day K (1996). The correlation between the intake of lutein, lycopene and beta-carotene from vegetables and fruits, and blood plasma concentrations in a group of women aged 50–65 years in the UK. Br J Nutr 75, 409–418.

    Article  CAS  Google Scholar 

  • Speek AJ, Schrijver J, Schreurs WHP (1984). Fluorometric determination of total vitamin C in whole blood by high-performance liquid chromatography with pre-column determination. J Chromatogr 305, 53–60.

    Article  CAS  Google Scholar 

  • Tanumihardjo SA (2004). Assessing retinol status: past, present and future. J Nutr 134, 290S–293S.

    Article  CAS  Google Scholar 

  • Thurnam DI, Davies JA, Crump BJ, Situnayake RD, Davis M (1986). The use of different lipid ratios for the measurement of α-tocopherol status. Ann Clin Biochem 23, 514–520.

    Article  Google Scholar 

  • WHO/UNICEF (2000). Indicators of VAD and their use in monitoring intervention programmes. In: WHO/NUT/96.10. World Health Organization: Geneva. pp 66.

  • Winbauer AN, Pingree SS, Nuttall KL (1999). Evaluating serum α-tocopherol (α-tocopherol) in terms of a lipid ratio. Ann Clin Lab Sci 29/3, 185–191.

    Google Scholar 

  • Winklhofer-Roob BM, van't Hof MA, Shmerling DH (1997). Reference values for plasma concentrations of α-tocopherol and A and carotenoids in a Swiss population from infancy to adulthood, adjusted for seasonal influences. Clin Chem 43, 146–153.

    CAS  PubMed  Google Scholar 

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Acknowledgements

The authors would like to thank Daniel Ruffieux who co-ordinated the SU.VI.MAX laboratory in Grenoble, Sandrine Dupuis and Sandrine Charrel who performed vitamin determinations.

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Authors and Affiliations

  1. Département de Biologie Intégrée Bâtiment B, CHU La Tronche BP, Grenoble, Cedex 9, France

    H Faure & A Favier

  2. U557 Inserm/Inra/Cnam, ISTNA Conservatoire National des Arts et Métiers 5, rue Vertbois, Paris, France

    P Preziosi, S Bertrais, P Galan & S Hercberg

  3. NVMC, Laboratory de Biochimie, UFR de médecine-pharmacie, Av. des maquis du Grésivaudan, La Tronche, France

    A-M Roussel

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  1. H Faure
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Correspondence to H Faure.

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Guarantors: S Hercberg and A Favier.

Contributors: SH and PG designed the study. HF, AF, and A-MR supervised and performed vitamin measurements. HF, PP and SB performed the statistical analysis.

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Faure, H., Preziosi, P., Roussel, AM. et al. Factors influencing blood concentration of retinol, α-tocopherol, vitamin C, and β-carotene in the French participants of the SU.VI.MAX trial. Eur J Clin Nutr 60, 706–717 (2006). https://doi.org/10.1038/sj.ejcn.1602372

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