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The frequency of Trp64Arg polymorphism of the β3-adrenergic receptor gene in healthy and obese Hungarian children and its association with cardiovascular risk factors

European Journal of Clinical Nutrition volume 59, pages 955–959 (2005)Cite this article

Abstract

Objective:

To estimate the frequency of Arg64 allele of the β3-adrenergic receptor (3-BAR) gene in healthy (H) and obese (O) Hungarian children, and to look for possible associations between this polymorphism and some clinical and metabolic characteristics of obese children.

Patients/methods:

In all, 147 healthy (male: 68) and 295 obese (male: 168) children were examined. The average age of the children in the two groups was 12.4±1.7 vs 12.6±3.2, respectively. Exon 1 of 3-BAR was amplified by polymerase chain reaction and the fragments were digested with BstN1. In obese children, oral glucose tolerance test was carried out and blood pressure (BP) was checked.

Results:

The frequency of Trp64Arg polymorphism in normal and obese Hungarian children was similar (H vs O: n=14/9.5% vs n=35/11.8%). Obese children carrying the Arg64 allele (n=35, male: 23) were compared to randomly chosen, obese children without the Arg64 allele (n=35, male: 20). A significant difference was found between the body weight (81.2±23.2 vs 75.6±17.7 kg; mean±s.d.; P<0.01), body fat (38.8±3.9 vs 36.5±2.3%; mean±s.d.; P<0.05), mean fasting insulin levels (31.4±16.7 vs 16.9±7.6 μIU/ml; P<0.001) and mean systolic BP values (125.2±10.1 vs 114.5±8.3 mmHg; P<0.001) of the two obese groups.

Conclusions:

The frequency of Trp64Arg polymorphism was similar in Hungary as compared to other European countries. Although the prevalence of this polymorphism was similar in H and O children, the presence of Arg64 allele seems to be associated with increased adiposity, elevated systolic BP and higher fasting insulin levels.

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Acknowledgements

This work was supported by Hungarian National Research Grant (OTKA T033066/2000), Hungarian Ministry of Welfare (ETT 113/2003) and Agency for Research Fund Management and Research Exploitation (BIO-00023/2002).

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Authors and Affiliations

  1. Department of Paediatrics, Medical Faculty, University of Pécs, Pécs, Hungary

    É Erhardt, K Csernus & D Molnár

  2. Department of Clinical Genetics and Child Development, Medical Faculty, University of Pécs, Pécs, Hungary

    M Czakó & Gy Kosztolányi

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  1. É Erhardt
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  2. M Czakó
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  4. D Molnár
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Correspondence to É Erhardt.

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Guarantor: D. Molnár.Contributors: M. Czakó, K. Csernus, Gy. Kosztolányi, and D. Molnár.

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Erhardt, É., Czakó, M., Csernus, K. et al. The frequency of Trp64Arg polymorphism of the β3-adrenergic receptor gene in healthy and obese Hungarian children and its association with cardiovascular risk factors. Eur J Clin Nutr 59, 955–959 (2005). https://doi.org/10.1038/sj.ejcn.1602164

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