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Lupin kernel fibre-enriched foods beneficially modify serum lipids in men



To examine the effect of a diet containing a novel legume food ingredient, Australian sweet lupin (Lupinus angustifolius) kernel fibre (LKFibre), compared to a control diet without the addition of LKFibre, on serum lipids in men.


Randomized crossover dietary intervention study.


Melbourne, Australia — Free-living men.


A total of 38 healthy males between the ages of 24 and 64 y completed the intervention.


Subjects consumed an LKFibre and a control diet for 1 month each. Both diets had the same background menus with seven additional experimental foods that either contained LKFibre or did not. Depending on energy intake, the LKFibre diet was designed to contain an additional 17 to 30 g/day fibre beyond that of the control diet.


Compared to the control diet, the LKFibre diet reduced total cholesterol (TC) (mean±s.e.m.; 4.5±1.7%; P=0.001), low-density lipoprotein cholesterol (LDL-C) (5.4±2.2%; P=0.001), TC: high-density lipoprotein cholesterol (HDL-C) (3.0±2.0%; P=0.006) and LDL-C:HDL-C (3.8±2.6%; P=0.003). No effects on HDL-C, triacylglycerols, glucose or insulin were observed.


Addition of LKFibre to the diet provided favourable changes to some serum lipid measures in men, which, combined with its high palatability, suggest this novel ingredient may be useful in the dietary reduction of coronary heart disease risk.


Grains Research and Development Corporation, Australian Research Council and the Department of Agriculture Western Australia.

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  • Anderson JW & Chen WL (1979): Plant fiber. Carbohydrate and lipid metabolism. Am. J. Clin. Nutr. 32, 346–363.

    Article  CAS  Google Scholar 

  • Anderson JW, Story L, Sieling B, Chen WJ, Petro MS & Story J (1984): Hypocholesterolemic effects of oat-bran or bean intake for hypercholesterolemic men. Am. J. Clin. Nutr. 40, 1146–1155.

    Article  CAS  Google Scholar 

  • Anderson KM, Castelli WP & Levy D (1987): Cholesterol and mortality. 30 years of follow-up from the Framingham study. JAMA 257, 2176–2180.

    Article  CAS  Google Scholar 

  • Anderson JW, Gustafson NJ, Spencer DB, Tietyen J & Bryant CA (1990): Serum lipid response of hypercholesterolemic men to single and divided doses of canned beans. Am. J. Clin. Nutr. 51, 1013–1019.

    Article  CAS  Google Scholar 

  • Anderson JW, Johnstone BM & Cook Newell ME (1995): Meta-analysis of the effects of soy protein intake on serum lipids. N. Engl. J. Med. 333, 276–282.

    Article  CAS  Google Scholar 

  • Anderson JW, Allgood LD, Lawrence A, Altringer LA, Jerdack GR, Hengehold DA & Morel JG (2000a): Cholesterol-lowering effects of psyllium intake adjunctive to diet therapy in men and women with hypercholesterolemia: meta-analysis of 8 controlled trials. Am. J. Clin. Nutr. 71, 472–479.

    Article  CAS  Google Scholar 

  • Anderson JW, Davidson MH, Blonde L, Brown WV, Howard WJ, Ginsberg H, Allgood LD & Weingand KW (2000b): Long-term cholesterol-lowering effects of psyllium as an adjunct to diet therapy in the treatment of hypercholesterolemia. Am. J. Clin. Nutr. 71, 1433–1438.

    Article  CAS  Google Scholar 

  • Anderson JW & Major AW (2002): Pulses and lipaemia, short- and long-term effect: potential in the prevention of cardiovascular disease. Br. J. Nutr. 88, S263–S271.

    Article  CAS  Google Scholar 

  • Anderson JW (2003): Diet first, then medication for hypercholesterolemia. JAMA 290, 531–533.

    Article  Google Scholar 

  • Anderson JW, Smith BM & Washnock CS (1999): Cardiovascular and renal benefits of dry bean and soybean intake. Am. J. Clin. Nutr. 70, 464–474.

    Article  Google Scholar 

  • AOAC (1990): Official Methods of Analysis. Washington, DC: Association of Official Analytical Chemist.

  • Archer BJ, Johnson SK, Devereux HM & Baxter AL (2004): Effect of fat replacement by inulin or lupin-kernel fibre on sausage patty acceptability, post-meal perceptions of satiety and food intake in men. Br. J. Nutr. 91, 591–599.

    Article  CAS  Google Scholar 

  • Ashton E & Ball M (2000): Effects of soy as tofu vs meat on lipoprotein concentrations. Eur. J. Clin. Nutr. 54, 14–19.

    Article  CAS  Google Scholar 

  • Bakhit RM, Klein BP, Essex Sorlie D, Ham JO, Erdman Jr JW & Potter SM (1994): Intake of 25 g of soybean protein with or without soybean fiber alters plasma lipids in men with elevated cholesterol concentrations. J. Nutr. 124, 213–222.

    Article  CAS  Google Scholar 

  • Bazzano LA, He J, Ogden LG, Loria C, Vupputuri S, Myers L & Whelton PK (2001): Legume consumption and risk of coronary heart disease in US men and women: NHANES I Epidemiologic Follow-up Study. Arch. Int. Med. 161, 2573–2578.

    Article  CAS  Google Scholar 

  • Brown L, Rosner B, Willett WW & Sacks FM (1999): Cholesterol-lowering effects of dietary fiber: a meta-analysis. Am. J. Clin. Nutr. 69, 30–42.

    Article  CAS  Google Scholar 

  • Burley VJ & Blundell JE (1990): Action of dietary fibre on the satiety cascade. In Dietary Fibre: Chemistry, Physiology, and Health Effects eds D Kritchevsky, C Bonfield & JW Anderson pp 227–246. New York: Plenum Press.

    Chapter  Google Scholar 

  • Cerda JJ, Robbins FL, Burgin CW, Baumgartner TG & Rice RW (1988): The effects of grapefruit pectin on patients at risk for coronary heart disease without altering diet or lifestyle. Clin. Cardiol. 11, 589–594.

    Article  CAS  Google Scholar 

  • Clark RL & Johnson SK (2002): Sensory acceptability of foods with added lupin (Lupinus angustifolius) kernel fiber using pre-set criteria. J. Food Sci. 67, 356–362.

    Article  CAS  Google Scholar 

  • Davidson MH, Dugan LD, Burns JH, Bova J, Story K & Drennan KB (1991): The hypocholesterolemic effects of beta-glucan in oatmeal and oat bran. A dose-controlled study. JAMA 265, 1833–1839.

    Article  CAS  Google Scholar 

  • Duane WC (1997): Effects of legume consumption on serum cholesterol, biliary lipids, and sterol metabolism in humans. J. Lipid Res. 38, 1120–1128.

    CAS  PubMed  Google Scholar 

  • Evans AJ, Cheung P & Cheetham NWH (1990): An evaluation of dietary fibre from Lupinus angustifolius. International Lupin Association sixth International Lupin Conference.

  • Evans AJ & Cheung PCK (1993): The carbohydrate composition of cotyledons and hulls of cultivars of Lupinus angustifolius from Western Australia. J. Sci. Food Agric. 61, 189–194.

    Article  CAS  Google Scholar 

  • Friedewald WT, Levy RI & Fredrickson DS (1972): Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18, 499–502.

    CAS  Google Scholar 

  • Fruhbeck G, Monreal I & Santidrian S (1997): Hormonal implications of the hypocholesterolemic effect of intake of field beans (Vicia faba L.) by young men with hypercholesterolemia. Am. J. Clin. Nutr. 66, 1452–1460.

    Article  CAS  Google Scholar 

  • Guillon F & Champ MM (2002): Carbohydrate fractions of legumes: uses in human nutrition and potential for health. Br. J. Nutr. 88, S293–S306.

    Article  CAS  Google Scholar 

  • Holt SHA, Brand Miller JC, Petocz P & Farmakalidis E (1995): A satiety index of common foods. Eur. J. Clin. Nutr. 49, 675–690.

    CAS  PubMed  Google Scholar 

  • Jenkins DJ, Wong GS, Patten R, Bird J, Hall M, Buckley GC, McGuire V, Reichert R & Little JA (1983): Leguminous seeds in the dietary management of hyperlipidemia. Am. J. Clin. Nutr. 38, 567–573.

    Article  CAS  Google Scholar 

  • Jenkins DJ, Kendall CW, Faulkner D, Vidgen E, Trautwein EA, Parker TL, Marchie A, Koumbridis G, Lapsley KG, Josse RG, Leiter LA & Connelly PW (2002a): A dietary portfolio approach to cholesterol reduction: combined effects of plant sterols, vegetable proteins, and viscous fibers in hypercholesterolemia. Metabolism 51, 1596–1604.

    Article  CAS  Google Scholar 

  • Jenkins DJ, Kendall CW, Vuksan V, Vidgen E, Parker T, Faulkner D, Mehling CC, Garsetti M, Testolin G, Cunnane SC, Ryan MA & Corey PN (2002b): Soluble fiber intake at a dose approved by the US Food and Drug Administration for a claim of health benefits: serum lipid risk factors for cardiovascular disease assessed in a randomized controlled crossover trial. Am. J. Clin. Nutr. 75, 834–839.

    Article  CAS  Google Scholar 

  • Jenkins DJ, Kendall CW, Marchie A, Faulkner DA, Wong JM, de Souza R, Emam A, Parker TL, Vidgen E, Lapsley KG, Trautwein EA, Josse RG, Leiter LA & Connelly PW (2003): Effects of a dietary portfolio of cholesterol-lowering foods vs lovastatin on serum lipids and C-reactive protein. JAMA 290, 502–510.

    Article  CAS  Google Scholar 

  • Johnson SK & Gray DM (1993): Ingredients derived from lupin — strong potential for a range of dietary fibre applications. IFI 5, 18–23.

    Google Scholar 

  • Johnson SK, McQuillan PL, Sin JH & Ball MJ (2003): Sensory acceptability of white bread with added Australian sweet lupin (Lupinus angustifolius) kernel fibre and its glycaemic and insulinaemic responses when eaten as a breakfast. J. Sci. Food Agric. 83, 1366–1372.

    Article  CAS  Google Scholar 

  • Kerckhoffs DA, Hornstra G & Mensink RP (2003): Cholesterol-lowering effect of beta-glucan from oat bran in mildly hypercholesterolemic subjects may decrease when beta-glucan is incorporated into bread and cookies. Am. J. Clin. Nutr. 78, 221–227.

    Article  CAS  Google Scholar 

  • Leterme P & Munoz LC (2002): Factors influencing pulse consumption in Latin America. Br. J. Nutr. 88, S251–S254.

    Article  CAS  Google Scholar 

  • Lo GS, Goldberg AP, Lim A, Grundhauser JJ, Anderson C & Schonfeld G (1986): Soy fiber improves lipid and carbohydrate metabolism in primary hyperlipidemic subjects. Atherosclerosis 62, 239–248.

    Article  CAS  Google Scholar 

  • LRC-CPPT (1984): The Lipid Research Clinics Coronary Primary Prevention Trial results. II. The relationship of reduction in incidence of coronary heart disease to cholesterol lowering. JAMA 251, 365–374.

  • Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF & Turner RC (1985): Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 28, 412–419.

    Article  CAS  Google Scholar 

  • Mensink RP, Zock PL, Kester AD & Katan MB (2003): Effects of dietary fatty acids and carbohydrates on the ratio of serum total to HDL cholesterol and on serum lipids and apolipoproteins: a meta-analysis of 60 controlled trials. Am. J. Clin. Nutr. 77, 1146–1155.

    Article  CAS  Google Scholar 

  • NCEP (2001): Executive Summary of The Third Report of The National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, And Treatment of High Blood Cholesterol In Adults (Adult Treatment Panel III). JAMA 285, 2486–2497.

  • NHF (2001): Lipid management guidelines—2001. National Heart Foundation of Australia, The Cardiac Society of Australia and New Zealand. Med. J. Aust. 175, S57–S85.

  • Petterson DS (1998): Composition and food uses of lupins. Lupins as Crop Plants: Biology, Production and Utilization eds JS Gladstones, CA Atkins & J Hamblin pp 353–384. Wallingford, Oxon: CAB International.

    Google Scholar 

  • Schweizer TF, Bekhechi AR, Koellreutter B, Reimann S, Pometta D & Bron BA (1983): Metabolic effects of dietary fiber from dehulled soybeans in humans. Am. J. Clin. Nutr. 38, 1–11.

    Article  CAS  Google Scholar 

  • Shepherd J, Cobbe SM, Ford I, Isles CG, Lorimer AR, MacFarlane PW, McKillop JH & Packard CJ (1995): Prevention of coronary heart disease with pravastatin in men with hypercholesterolemia. West of Scotland Coronary Prevention Study Group. N. Engl. J. Med. 333, 1301–1307.

    Article  CAS  Google Scholar 

  • Shorey RL, Day PJ, Willis RA, Lo GS & Steinke FH (1985): Effects of soybean polysaccharide on plasma lipids. J. Am. Diet. Assoc. 85, 1461–1465.

    CAS  PubMed  Google Scholar 

  • Stamler J, Wentworth D & Neaton JD (1986): Is relationship between serum cholesterol and risk of premature death from coronary heart disease continuous and graded? Findings in 356,222 primary screenees of the Multiple Risk Factor Intervention Trial (MRFIT). JAMA 256, 2823–2828.

    Article  CAS  Google Scholar 

  • Tsai AC, Mott EL, Owen GM, Bennick MR, Lo GS & Steinke FH (1983): Effects of soy polysaccharide on gastrointestinal functions, nutrient balance, steroid excretions, glucose tolerance, serum lipids, and other parameters in humans. Am. J. Clin. Nutr. 38, 504–511.

    Article  CAS  Google Scholar 

  • Turnbull C, Baxter A & Johnson SK (2002): In vitro hydration and fermentation properties of lupin kernel fibre compared to commercial dietary fibres under simulated human gastrointestinal conditions. Functional Foods Through Innovation to Market.

  • Yu Poth S, Zhao G, Etherton T, Naglak M, Jonnalagadda S & Kris Etherton PM (1999): Effects of the National Cholesterol Education Program's Step I and Step II dietary intervention programs on cardiovascular disease risk factors: a meta-analysis. Am. J. Clin. Nutr. 69, 632–646.

    Article  CAS  Google Scholar 

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We are grateful to Food Science Australia for manufacturing the LKFibre and to George Weston Foods for producing the experimental food products and conducting nutritional analyses of these foods. We thank Carl Gibbons for database construction, Iain Robertson for conducting the statistical analysis of biochemical parameters and Damien Jolley for assisting with statistical interpretation. We are indebted to the study participants for their commitment to the study protocol.

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Correspondence to S K Johnson.

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Guarantors: SK Johnson.

Contributors: RSH, SKJ and MJB were responsible for the design of this study; RSH and ALB performed subject recruitment, selection, development of research diets and management of feeding trials; RSH and SKJ interpreted the data and drafted the manuscript and MJB reviewed and edited the manuscript.

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Hall, R., Johnson, S., Baxter, A. et al. Lupin kernel fibre-enriched foods beneficially modify serum lipids in men. Eur J Clin Nutr 59, 325–333 (2005).

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  • lupin
  • fibre
  • legume
  • cholesterol
  • lipid

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