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Plasma and lung macrophage responsiveness to carotenoid supplementation and ozone exposure in humans

European Journal of Clinical Nutrition volume 58, pages 1571–1579 (2004)Cite this article

Abstract

Objective: To examine the effect of ozone exposure and vegetable juice supplementation on plasma and lung macrophage concentrations of carotenoids.

Design: A randomized trial.

Setting: Subjects were exposed to ambient air prior to antioxidant supplementation and to ozone after antioxidant supplementation or placebo. Exposures occurred while exercising intermittently in a controlled metabolic chamber at the Human Studies Division, US EPA.

Subjects: In all, 23 healthy subjects between ages of 18 and 35 y.

Interventions: Subjects consumed a low fruit and vegetable diet for 3 weeks. After the first week, subjects underwent a sham exposure to filtered air with exercise, followed by bronchoalveolar lavage (BAL). Subjects were randomly assigned into supplement (one can vegetable juice, vitamins C and E daily) or placebo (orange soda, placebo pill daily) groups for 2 weeks. After the 2-week intervention, subjects were exposed to 0.4 ppm (784 μg/m3) ozone for 2 h with exercise followed by BAL. Blood samples were drawn before, immediately after and 3 h postexposure on each exposure day. The concentrations of nine carotenoids were determined by HPLC in BAL macrophages and plasma samples.

Results: Plasma concentrations of all the carotenoids that were present in the vegetable juice (except cis-beta-carotene) increased significantly in the supplemented group. Lung macrophage α-carotene concentrations increased significantly, lycopene isomers increased slightly, and all other carotenoids decreased (nonsignificantly) in the supplementation group following the intervention. Ozone exposure resulted in decreases in several carotenoids in plasma of the placebo group, but not in the supplemented group.

Conclusions: Lung macrophage concentrations of carotenoids can be manipulated by diet. Ozone is a potent environmental oxidant that appears to reduce plasma carotenoids in nonsupplemented individuals.

Sponsorship: US Environmental Protection Agency, Campbell's Soup Company.

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Authors and Affiliations

  1. Department of Nutrition, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    S Steck-Scott & L Arab

  2. Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    L Arab

  3. Craft Technologies, Inc., Wilson, NC, USA

    N E Craft

  4. NHEERL, US EPA, Chapel Hill, NC, USA

    J M Samet

Authors
  1. S Steck-Scott
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  2. L Arab
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  3. N E Craft
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  4. J M Samet
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Contributions

Guarantor: S Steck-Scott.

Contributors: SSS was nutrition coordinator, data analyst and main writer; LA contributed to the planning, analyzing, interpretation of results and writing; NC performed laboratory analyses of carotenoids; JS was principal investigator of the ANTIOZ study. All authors read and commented on the manuscript.

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Correspondence to S Steck-Scott.

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Steck-Scott, S., Arab, L., Craft, N. et al. Plasma and lung macrophage responsiveness to carotenoid supplementation and ozone exposure in humans. Eur J Clin Nutr 58, 1571–1579 (2004). https://doi.org/10.1038/sj.ejcn.1601988

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