Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Effects of fish oil supplementation on apolipoprotein B100 production and lipoprotein metabolism in normolipidaemic males

Abstract

Objective: To evaluate the effects of four weeks of fish oil supplementation on apolipoprotein B100 production and lipoprotein metabolism in normolipidaemic males.

Design and subjects: Very low density lipoprotein (VLDL) apolipoprotein B100 (apoB100) kinetics in ten healthy, white males, aged 22–43 y (mean 32 y) were investigated using a 13C-leucine technique and gas chromatography-mass spectrometry before and after fish oil supplementation.

Intervention: All subjects received 10 g (1.8 g EPA, 1.2 g DHA)/d of fish oil concentrate for four weeks.

Results: Fish oil supplementation resulted in a decrease of total plasma VLDL (mean±s.d. 1.11±0.41 vs 0.87±0.28 mmol/l, P<0.05) and triacylglycerol concentrations (0.74±0.27 vs 0.48±0.21 mmol/l, P<0.01). VLDL apoB100 pool size was decreased without alteration of the fractional synthetic rate but a significant decrease of apoB100 production (2.23±0.90 vs 1.54±0.52 mg/dl/h, P<0.02). Following fish oil supplementation plasma concentrations of glucose and insulin as well as lipoprotein and hepatic lipase activities were unchanged. Fasting plasma concentrations of non-esterified fatty acid (NEFA) were decreased (0.45±0.12 vs 0.33±0.10 mmol/l, P<0.05).

Conclusions: Dietary supplementation with fish oil in healthy males results in decreased VLDL-triacylglycerol concentrations through a decrease in VLDL particle synthesis. The decrease in NEFA substrate supply also contributes.

Sponsorship: Fish oil (Maxepa®) was supplied by Seven Seas Ltd, Hull, England, free of charge. Dr OAF Bodamer was funded by the `Deutsche Forschungsgemeinschaft' (Bo 1193/1-2).

Author information

Affiliations

Authors

Corresponding author

Correspondence to OAF Bodamer.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Bordin, P., Bodamer, O., Venkatesan, S. et al. Effects of fish oil supplementation on apolipoprotein B100 production and lipoprotein metabolism in normolipidaemic males. Eur J Clin Nutr 52, 104–109 (1998). https://doi.org/10.1038/sj.ejcn.1600522

Download citation

Keywords

  • fish oil
  • n-3 fatty acids
  • apoB100
  • lipoprotein
  • mass spectrometry
  • stable isotopes

Further reading

Search

Quick links