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  • Oncogenomics
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Identification of tumor markers and differentiation markers for molecular diagnosis of lung adenocarcinoma

Oncogene volume 25, pages 4245–4255 (2006)Cite this article

Abstract

To identify tumor markers and differentiation markers for lung adenocarcinoma (AdC), we analysed expression profiles of 14 500 genes against three cases of type II alveolar epithelial cells, bronchiolar epithelial cells, and bronchial epithelial cells, respectively, and 10 cases of AdC cells isolated by laser capture microdissection. Hierarchical clustering analysis indicated that AdC cells and noncancerous lung epithelial cells are significantly different in their expression profiles, and that different sets of differentiation markers are expressed among alveolar, bronchiolar and bronchial epithelial cells. Nine genes were identified as being highly expressed in AdC cells, but not expressed in noncancerous lung epithelial cells. Sixteen genes were identified as differentiation markers for lung epithelial cells. Real-time RT–PCR analysis of 45 lung AdC cases further revealed that expression of four tumor markers in AdC cells was significantly higher than that in noncancerous lung cells and that expression of ten differentiation markers was retained in a considerable fraction of lung AdC cases. Five tumor markers and seven differentiation markers were not expressed in peripheral blood cells. Similarities and differences in expression profiles between normal epithelial cells from different lung respiratory compartments and AdC cells demonstrated in this study will be informative for the molecular diagnosis of lung AdC.

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Acknowledgements

This work was supported in part by Grants-in-Aid from the Ministry of Health, Labor and Welfare of Japan for the 2nd- and 3rd-term Comprehensive 10-year Strategy for Cancer Control, for Cancer Research (10-4 and 16-1), and for the Program for Promotion of Fundamental Studies in Health Sciences of the Organization for Pharmaceutical Safety and Research. KK and NN were awardees of the Research Resident Fellowship from the Foundation for Promotion of Cancer Research during this study.

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Author notes

  1. N Nakamura, K Kobayashi and M Nakamoto: These authors contributed equally to this work.

Authors and Affiliations

  1. Biology Division, National Cancer Center Research Institute, Tokyo, Japan

    N Nakamura, K Kobayashi, M Nakamoto, T Kohno & J Yokota

  2. Genetics Division, National Cancer Center Research Institute, Tokyo, Japan

    H Sasaki

  3. Pathology Division, National Cancer Center Research Institute, Tokyo, Japan

    Y Matsuno

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  1. N Nakamura
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  2. K Kobayashi
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Correspondence to J Yokota.

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Supplementary Information accompanies the paper on Oncogene website (http://www.nature.com/onc)

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Nakamura, N., Kobayashi, K., Nakamoto, M. et al. Identification of tumor markers and differentiation markers for molecular diagnosis of lung adenocarcinoma. Oncogene 25, 4245–4255 (2006). https://doi.org/10.1038/sj.onc.1209442

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