Abstract
The Clathrin Assembly Lymphoid Myeloid leukemia gene (CALM or PICALM) was first identified as the fusion partner of AF10 in the t(10;11)(p13;q14) translocation, which is observed in acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL) and malignant lymphoma. The CALM/AF10 fusion protein plays a crucial role in t(10;11)(p13;q14) associated leukemogenesis. Using the N-terminal half of CALM as a bait in a yeast two-hybrid screen, a novel protein named CATS (CALM interacting protein expressed in thymus and spleen) was identified. Multiple tissue Northern blot analysis showed predominant expression of CATS in thymus, spleen and colon. CATS codes for two protein isoforms of 238 and 248 amino acids (aa). The interaction between CALM and CATS could be confirmed using pull down assays, co-immunoprecipitation and colocalization experiments. The CATS interaction domain of CALM was mapped to aa 221–335 of CALM. This domain is contained in the CALM/AF10 fusion protein. CATS localizes to the nucleus and shows a preference for nucleoli. Expression of CATS was able to markedly increase the nuclear localization of CALM and of the leukemogenic fusion protein CALM/AF10. The possible implications of these findings for CALM/AF10-mediated leukemogenesis are discussed.
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References
Abdou SM, Jadayel DM, Min T, Swansbury GJ, Dainton MG, Jafer O et al. (2002). Leuk Lymphoma 43: 89–95.
Asnafi V, Radford-Weiss I, Dastugue N, Bayle C, Leboeuf D, Charrin C et al. (2003). Blood 102: 1000–1006.
Ayton PM, Cleary ML . (2001). Oncogene 20: 5695–5707.
Bohlander SK, Muschinsky V, Schrader K, Siebert R, Schlegelberger B, Harder L et al. (2000). Leukemia 14: 93–99.
Carlson KM, Vignon C, Bohlander S, Martinez-Climent JA, Le Beau MM, Rowley JD . (2000). Leukemia 14: 100–104.
Chao MM, Walker AC, Pendergast MB, Bohlander SK, Wechsler AC . (2004). Blood 104: 922a–923a.
Chaplin T, Ayton P, Bernard OA, Saha V, Della Valle V, Hillion J et al. (1995). Blood 85: 1435–1441.
De Camilli P, Chen H, Hyman J, Panepucci E, Bateman A, Brunger AT . (2002). FEBS Lett 513: 11–18.
Delabesse E, Dik WA, Brahim W, Braun C, Asnafi V, Van Dongen JJM et al. (2004). Blood 104: 315a.
Deshpande A, Krause A, Cusan M, Wolf E, Hiddemann W, Humphries RK et al. (2003). Blood 102: 216a.
Di Fiore PP, De Camilli P . (2001). Cell 106: 1–4.
DiMartino JF, Ayton PM, Chen EH, Naftzger CC, Young BD, Cleary ML . (2002). Blood 99: 3780–3785.
Dreyling MH, Martinez-Climent JA, Zheng M, Mao J, Rowley JD, Bohlander SK . (1996). Proc Natl Acad Sci USA 93: 4804–4809.
Dreyling MH, Schrader K, Fonatsch C, Schlegelberger B, Haase D, Schoch C et al. (1998). Blood 91: 4662–4667.
Evans PR, Owen DJ . (2002). Curr Opin Struct Biol 12: 814–821.
Ford MG, Mills IG, Peter BJ, Vallis Y, Praefcke GJ, Evans PR et al. (2002). Nature 419: 361–366.
Ford MG, Pearse BM, Higgins MK, Vallis Y, Owen DJ, Gibson A et al. (2001). Science 291: 1051–1055.
Huang F, Khvorova A, Marshall W, Sorkin A . (2004). J Biol Chem 279: 16657–16661.
Jones LK, Chaplin T, Shankar A, Neat M, Patel N, Samuel DP et al. (2001). Leukemia 15: 910–914.
Kalthoff C, Alves J, Urbanke C, Knorr R, Ungewickell EJ . (2002). J Biol Chem 277: 8209–8216.
Klebig ML, Wall MD, Potter MD, Rowe EL, Carpenter DA, Rinchik EM . (2003). Proc Natl Acad Sci USA 100: 8360–8365.
Kobayashi H, Hosoda F, Maseki N, Sakurai M, Imashuku S, Ohki M et al. (1997). Genes Chromosomes Cancer 20: 253–259.
Krause A, Kohlmann A, Haferlach T, Schoch C, Schnittger S, Mecucci C et al. (2004). Blood 104: 791a.
Kumon K, Kobayashi H, Maseki N, Sakashita A, Sakurai M, Tanizawa A et al. (1999). Genes Chromosomes Cancer 25: 33–39.
Morris SA, Schroder S, Plessmann U, Weber K, Ungewickell E . (1993). EMBO J 12: 667–675.
Nakamura F, Maki K, Arai Y, Nakamura Y, Mitani K . (2003). Am J Hematol 72: 138–142.
Narita M, Shimizu K, Hayashi Y, Taki T, Taniwaki M, Hosoda F et al. (1999). Br J Haematol 105: 928–937.
Netzer C, Rieger L, Brero A, Zhang CD, Hinzke M, Kohlhase J et al. (2001). Hum Mol Genet 10: 3017–3024.
Nonet ML, Holgado AM, Brewer F, Serpe CJ, Norbeck BA, Holleran J et al. (1999). Mol Biol Cell 10: 2343–2360.
Perrin L, Bloyer S, Ferraz C, Agrawal N, Sinha P, Dura JM . (2003). Mol Cell Biol 23: 119–130.
Saha V, Chaplin T, Gregorini A, Ayton P, Young BD . (1995). Proc Natl Acad Sci USA 92: 9737–9741.
Salmon-Nguyen F, Busson M, Daniel M, Leblanc T, Bernard OA, Berger R . (2000). Cancer Genet Cytogenet 122: 137–140.
Schlosser I, Holzel M, Murnseer M, Burtscher H, Weidle UH, Eick D . (2003). Nucleic Acids Res 31: 6148–6156.
Silliman CC, McGavran L, Wei Q, Miller LA, Li S, Hunger SP . (1998). Leukemia 12: 1404–1410.
Stahelin RV, Long F, Peter BJ, Murray D, De Camilli P, McMahon HT et al. (2003). J Biol Chem 278: 28993–28999.
Tebar F, Bohlander SK, Sorkin A . (1999). Mol Biol Cell 10: 2687–2702.
Tsai RY, McKay RD . (2002). Genes Dev 16: 2991–3003.
Vecchi M, Polo S, Poupon V, van de Loo JW, Benmerah A, Di Fiore PP . (2001). J Cell Biol 153: 1511–1517.
Zhang B, Koh YH, Beckstead RB, Budnik V, Ganetzky B, Bellen HJ . (1998). Neuron 21: 1465–1475.
Acknowledgements
We thank Michael Hölzel for the YFP-nucleostemin construct and Sigrun Bartels for technical assistance. This research was supported by the Nationales Genomforschungsnetz (NGFN, BMBF), the German Academic Exchange Service (DAAD), the Deutsche Forschungsgemeinschaft (DFG) and the Deutsche José Carreras Leukämie-Stiftung e.V. (DJCLS).
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Archangelo, L., Gläsner, J., Krause, A. et al. The novel CALM interactor CATS influences the subcellular localization of the leukemogenic fusion protein CALM/AF10. Oncogene 25, 4099–4109 (2006). https://doi.org/10.1038/sj.onc.1209438
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DOI: https://doi.org/10.1038/sj.onc.1209438
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