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Possible role of Rho/Rhotekin signaling in mammalian septin organization

Oncogene volume 24, pages 7064–7072 (2005)Cite this article

Abstract

There is growing evidence for crosstalk between septin filaments and actin cytoskeleton which is regulated by Rho family of GTPases. Here we show that active Rho disrupts septin filament structures in rat embryonic fibroblast REF52 cells. Among Rho effector molecules tested, Rhotekin induced morphological changes of septin filaments similar to those by activated Rho. The center region of Rhotekin was sufficient for the septin reorganization in the cells, and likely to interact indirectly with the C-terminal half of a septin Sept9b, where a GTPase domain is located. Rhotekin and Sept9b are colocalized mainly in perinuclear regions in serum-starved REF52 cells. Upon stimulation with lysophosphatidic acid, they translocated to actin stress fibers in 10 min and then redistributed again to cytoplasm after 90 min treatment. In neuroblastoma Neuro2a cells, Rhotekin and Sept9b were enriched in the tip of neurites, a location where cortical actin reorganization is induced upon stimulation with lysophosphatidic acid. Taken together, we propose that Rhotekin is a novel regulator organizing mammalian septin structures and provide a new link between the septin and Rho-signaling.

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Acknowledgements

This work was supported in part by grants-in-aid for scientific research from Ministry of Education, Science, Technology, Sports and Culture of Japan and by Aichi Cancer Research Foundation.

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Authors and Affiliations

  1. Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya-cho, Kasugai, 480-0392, Japan

    Hidenori Ito, Ikuko Iwamoto, Rika Morishita, Tomiko Asano & Koh-ichi Nagata

  2. Gifu International Institute of Biotechnology, 1-1 Nakafudogaoka, Gifu, 504-0838, Japan

    Yoshinori Nozawa

  3. Department of Pharmacology, Kyoto University Faculty of Medicine, Yoshida, Sakyo-ku, 606-8501, Kyoto, Japan

    Shuh Narumiya

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  1. Hidenori Ito
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  2. Ikuko Iwamoto
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Correspondence to Koh-ichi Nagata.

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Ito, H., Iwamoto, I., Morishita, R. et al. Possible role of Rho/Rhotekin signaling in mammalian septin organization. Oncogene 24, 7064–7072 (2005). https://doi.org/10.1038/sj.onc.1208862

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