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Epigenetic silencing of TCEAL7 (Bex4) in ovarian cancer

Oncogene volume 24, pages 5089–5100 (2005)Cite this article

Abstract

Epigenetic silencing by hypermethylation of CpGs represents a mechanism of inactivation of tumor suppressors. Here we report on the cloning of a novel candidate tumor suppressor gene TCEAL7 inactivated by methylation in ovarian cancer. TCEAL codes for a 1.35 kb transcript that was previously reported to be downregulated in ovarian cancer by cDNA microarray and suppression subtraction cDNA (SSH) analyses. This report focuses on the elucidation of mechanisms associated with TCEAL7 downregulation. Expression of TCEAL7 is downregulated in a majority of ovarian tumors and cancer cell lines but induced by 5-aza-2′-deoxycytidine treatment in a dose-dependant manner, implicating methylation as a mechanism of TCEAL7 inactivation. Sequence analyses of bisufite-modified genomic DNA from somatic cell hybrids with either the active or the inactive human X chromosome reveal that TCEAL7 is subjected to X chromosome inactivation. Loss of TCEAL7 expression in primary tumors and cell lines correlates with methylation of a CpG site within the promoter. In vitro methylation of the CpG site suppresses promoter activity whereas selective demethylation of the SmaI site attenuates the suppression. Finally, re-expression of TCEAL7 in cancer cell lines induces cell death and reduces colony formation efficiency. These data implicate TCEAL7 as a cell death regulatory protein that is frequently inactivated in ovarian cancers, and suggest that it may function as a tumor suppressor.

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Acknowledgements

We thank Dr Kimberly R Kalli (Mayo Clinic, Rochester) for the short-term cultures of OSE and Dr Mariano Rocchi (University of Bari, Italy) for the somatic cell hybrids used in this study. This work was supported by Department of Defense Grant W81XWH-04-1-0085 (to VS) and DAMD17-02-1-0473 (to VS, DIS, SHK, and LCH) and the Mayo Foundation (to VS).

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Authors and Affiliations

  1. Division of Experimental Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic/Foundation, 200 First Street, SW, Rochester, 55905, MN, USA

    Jeremy Chien, Julie Staub, Rajeswari Avula, Heyu Zhang, Wanguo Liu, David I Smith & Viji Shridhar

  2. Division of Medical Oncology, Department of Oncology, Oncology Research, Mayo Clinic/Foundation, 200 First Street, SW, Rochester, 55905, MN, USA

    Lynn C Hartmann

  3. Department of Oncology Research, Mayo Clinic/Foundation, 200 First Street, SW, Rochester, 55905, MN, USA

    Scott H Kaufmann

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  1. Jeremy Chien
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Correspondence to Viji Shridhar.

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Chien, J., Staub, J., Avula, R. et al. Epigenetic silencing of TCEAL7 (Bex4) in ovarian cancer. Oncogene 24, 5089–5100 (2005). https://doi.org/10.1038/sj.onc.1208700

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