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Huntingtin interacting protein 14 is an oncogenic human protein: palmitoyl acyltransferase

Oncogene volume 23, pages 9230–9237 (2004)Cite this article

Abstract

Protein palmitoyltransferases (PATs) represent an exciting new target for anticancer drug design due to their pivotal roles in the subcellular localization of a number of oncogenes. We show that the Huntingtin interacting protein 14 (HIP14) is a PAT with a preference for the farnesyl-dependent palmitoylation motif found in H- and N-RAS. Characterization of HIP14 in mouse cells has revealed that it has the ability to induce colony formation in cell culture, anchorage-independent growth, and tumors in mice. Activity of the enzyme and its ability to transform cells is dependent on critical residues in the active site of the enzyme.

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Acknowledgements

We thank Dwayne Dexter for assistance in obtaining the HIP14 clone. We are also grateful to Lynn Maines for his assistance in the preparation of the manuscript. This work was supported by NIH Grant No. R01 CA75248 to CDS.

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  1. Department of Pharmacology, H078, Penn State College of Medicine, 500 University Drive Hershey, 17033, PA, USA

    Charles E Ducker, Erin M Stettler, Kevin J French, John J Upson & Charles D Smith

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  1. Charles E Ducker
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Correspondence to Charles D Smith.

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Ducker, C., Stettler, E., French, K. et al. Huntingtin interacting protein 14 is an oncogenic human protein: palmitoyl acyltransferase. Oncogene 23, 9230–9237 (2004). https://doi.org/10.1038/sj.onc.1208171

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