Abstract
Nonsteroidal anti-inflammatory drugs (NSAIDs) such as aspirin have been shown to suppress transcription factor NF-κB, which controls the expression of genes such as cyclooxygenase (COX)-2 and cyclin D1, leading to inhibition of proliferation of tumor cells. There is no systematic study as to how these drugs differ in their ability to suppress NF-κB activation and NF-κB-regulated gene expression or cell proliferation. In the present study, we investigated the effect of almost a dozen different commonly used NSAIDs on tumor necrosis factor (TNF)-induced NF-κB activation and NF-κB-regulated gene products, and on cell proliferation. Dexamethasone, an anti-inflammatory steroid, was included for comparison with NSAIDs. As indicated by DNA binding, none of the drugs alone activated NF-κB. All compounds inhibited TNF-induced NF-κB activation, but with highly variable efficacy. The 50% inhibitory concentration required was 5.67, 3.49, 3.03, 1.25, 0.94, 0.60, 0.38, 0.084, 0.043, 0.027, 0.024, and 0.010 mM for aspirin, ibuprofen, sulindac, phenylbutazone, naproxen, indomethacin, diclofenac, resveratrol, curcumin, dexamethasone, celecoxib, and tamoxifen, respectively. All drugs inhibited IκBα kinase and suppressed IκBα degradation and NF-κB-regulated reporter gene expression. They also suppressed NF-κB-regulated COX-2 and cyclin D1 protein expression in a dose-dependent manner. All compounds inhibited the proliferation of tumor cells, with 50% inhibitory concentrations of 6.09, 1.12, 0.65, 0.49, 1.01, 0.19, 0.36, 0.012, 0.016, 0.047, 0.013, and 0.008 mM for aspirin, ibuprofen, sulindac, phenylbutazone, naproxen, indomethacin, diclofenac, resveratrol, curcumin, dexamethasone, celecoxib, and tamoxifen, respectively. Overall these results indicate that aspirin and ibuprofen are least potent, while resveratrol, curcumin, celecoxib, and tamoxifen are the most potent anti-inflammatory and antiproliferative agents of those we studied.
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Abbreviations
- NSAIDs:
-
nonsteroidal anti-inflammatory drugs
- TNF:
-
tumor necrosis factor
- IκB:
-
inhibitory subunit of NF-κB
- IKK:
-
IκBα kinase
- EMSA:
-
electrophoretic mobility shift assays
- SEAP:
-
secretory alkaline phosphatase
- COX:
-
cyclooxygenase
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Acknowledgements
We thank Mr Walter Pagel for carefully proof-reading the manuscript and providing valuable comments. Dr Aggarwal is a Ransom Horne Jr, Distinguished Professor of Cancer Research. This work was supported partially by the Clayton Foundation for Research (to BBA), Department of Defense US Army Breast Cancer Research Program Grant BC010610 (to BBA), a PO1 Grant (CA91844) from the National Institutes of Health on Lung Cancer Chemoprevention (to BBA), and a P50 Head and Neck SPORE Grant from the National Institutes of Health (to BBA).
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Takada, Y., Bhardwaj, A., Potdar, P. et al. Nonsteroidal anti-inflammatory agents differ in their ability to suppress NF-κB activation, inhibition of expression of cyclooxygenase-2 and cyclin D1, and abrogation of tumor cell proliferation. Oncogene 23, 9247–9258 (2004). https://doi.org/10.1038/sj.onc.1208169
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DOI: https://doi.org/10.1038/sj.onc.1208169
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