Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

Immunological and pathobiological roles of fibulin-1 in breast cancer

Oncogene volume 23, pages 2153–2160 (2004)Cite this article

A Corrigendum to this article was published on 19 August 2004

Abstract

Fibulin-1 (Fbln-1) is an immunogenic breast cancer-related glycoprotein identified by serological analysis of cDNA expression library (SEREX) strategy. Here, we show that dendritic cells from two breast cancer patients elicited a CD4+-mediated T-cell response to Fbln-1 presentation. In both patients, an antibody response to Fbln-1 was also found. By contrast, a Fbln-1-seronegative patient and a weakly seropositive patient demonstrated no such T-cell response. Analysis of human breast cancers for Fbln-1 RNA and protein expression revealed the presence of Fbln-1C and -1D variants. Fbln-1 was detected in the cytoplasm and at the cell surface of different human breast carcinoma cell lines. Immunohistochemical analysis of 528 archival primary breast carcinomas showed the expression of Fbln-1 in 35% of the cases. When the immunohistochemical findings were compared against pathobiological information associated with each specimen, an inverse relationship between Fbln-1 and cathepsin D expression was observed (P=0.04). Furthermore, even though long-term survival was similar between Fbln-1-positive and -negative cases, the survival of Fbln-1-positive cases improved when a lymphoid infiltrate was present at the tumour site. Taken together, our findings of an Fbln-1-specific immunity and the improved survival associated with Fbln-1 expression in the presence of lymphoid infiltration point to a role of Fbln-1 in tumour immunosurveillance.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5

Similar content being viewed by others

References

  • Argraves WS, Tran H, Burgess WH and Dickerson K . (1990). J. Cell Biol., 111, 3155–3164.

  • Brichard V, Van Pel A, Wölfel T, Wölfel C, De Plaen E, Lethé B, Coulie P and Boon T . (1993). J. Exp. Med., 178, 489–495.

  • Clinton GM, Rougeot C, Derancourt J, Roger P, Defrenne A, Godyna S, Argraves WS and Rochefort H . (1996). Proc. Natl. Acad. Sci. USA, 93, 316–320.

  • Du M, Fan X, Hong E and Chen JJ . (2002). Biochem. Biophys. Res. Commun., 296, 962–969.

  • Forti S, Scanlan MJ, Invernizzi AM, Castiglioni F, Pupa S, Agresti R, Fontanelli R, Morelli D, Old LJ, Pupa SM and Ménard S . (2002). Breast Cancer Res. Treat., 73, 245–256.

  • Greene LM, Twal WO, McDermott EW, Hill AD, O'Higgins NJ, McCann AH, Dervan PA, Duffy MJ and Argraves WS . (2003). Br. J. Cancer, 88, 871–878.

  • Hayashido Y, Lucas A, Rougeot C, Godyna S, Argraves WS and Rochefort H . (1998). Int. J. Cancer, 75, 654–658.

  • Jager E, Chen YT, Drijfhout JW, Karbach J, Ringhoffer M, Jager D, Arand M, Wada H, Noguchi Y, Stockert E, Old LJ and Knuth A . (1998). J. Exp. Med., 187, 265–270.

  • Jager E, Nagata Y, Gnjatic S, Wada H, Stockert E, Karbach J, Dunbar PR, Lee SY, Jungbluth A, Jager D, Arand M, Ritter G, Cerundolo V, Dupont B, Chen YT, Old LJ and Knuth A . (2000). Proc. Natl. Acad. Sci. U.S.A, 97, 4760–4765.

  • Liu YJ, Kanzler H, Soumelis V and Gilliet M . (2001). Natl. Immunol., 2, 585–589.

  • Lutz M and Schuler G . (2002). Trends Immunol., 23, 445.

  • Martignone S, Pellegrini R, Villa E, Tandon NN, Mastroianni A, Tagliabue E, Ménard S and Colnaghi MI . (1992). Clin. Exp. Met., 10, 379–386.

  • Ménard S, Casalini P, Tomasic G, Pilotti S, Cascinelli N, Bufalino R, Perrone F, Longhi C, Rilke F and Colnaghi MI . (1999). Breast Cancer Res. Treat., 55, 169–177.

  • Moll F, Katsaros D, Lazennec G, Hellio N, Roger P, Giacalone PL, Chalbos D, Maudelonde T, Rochefort H and Pujol P . (2002). Oncogene, 21, 1097–1107.

  • Nakatsura T, Senju S, Ito M, Nishimura Y and Itoh K . (2002). Eur. J. Immunol., 32, 826–836.

  • Niu Y, Fu X, Lv A, Fan Y and Wang Y . (2002). Int. J. Cancer, 98, 754–760.

  • Old LJ and Chen YT . (1998). J. Exp. Med., 187, 1163–1167.

  • Parmiani G, Castelli C, Dalerba P, Mortarini R, Rivoltini L, Marincola FM and Anichini A . (2002). J. Natl. Cancer Inst., 94, 805–818.

  • Pereira H, Pinder SE, Sibbering DM, Galea MH, Elston CW, Blamey RW, Robertson JFR and Ellis IO . (1995). Histopathology, 27, 219–226.

  • Pupa SM, Forti S, Balsari A and Ménard S . (2002a). Ann. Oncol., 13, 483.

  • Pupa SM, Forti S, Invernizzi AM, Giovanazzi R, Twal WO, Argraves WS and Ménard S . (2003). J. Cell. Biochem., 89, 647–652.

  • Pupa SM, Ménard S, Forti S and Tagliabue E . (2002b). J. Cell. Physiol., 192, 259–267.

  • Qing J, Maher VM, Tran H, Argraves WS, Dunstan RW and McCormick JJ . (1997). Oncogene, 15, 2159–2168.

  • Roark EF, Keene DR, Haundenschild CC, Godyna S, Little CD and Argraves WS . (1995). J. Histochem. Cytochem., 43, 401–411.

  • Roger P, Pujol P, Lucas A, Baldet P and Rochefort H . (1998). Am. J. Pathol., 153, 1579–1588.

  • Rosenberg SA . (2001). Nature, 411, 380–384.

  • Sahin U, Tureci O and Pfreundschuh M . (1997). Curr. Opin. Immunol., 9, 709–716.

  • Sahin U, Tureci O, Schmitt H, Cochlovius B, Johannes T, Schmits R, Stenner F, Luo G, Schobert I and Pfreundschuh M . (1995). Proc. Natl. Acad. Sci. USA, 92, 11810–11813.

  • Sallusto F and Lanzavecchia A . (1994). J. Exp. Med., 179, 1109–1118.

  • Struss AK, Romeike BF, Munnia A, Nastainczyk W, Steudel WI, Konig J, Ohgaki H, Feiden W, Fischer U and Meese E . (2001). Oncogene, 20, 4107–4114.

  • Tran H, Mattei M, Godyna S and Argraves WS . (1997). Matrix Biol., 15, 479–493.

  • Twal WO, Czirok A, Hegedus B, Knaak C, Chintalapudi MR, Okagawa H, Sugi Y and Argraves WS . (2001). J. Cell Sci., 114, 4587–4598.

  • Van der Bruggen P, Traversari C, Chomez P, Lurquin C, De Plaen E, Van den Eynde B, Knuth A and Boon T . (1991). Science, 254, 1643–1647.

Download references

Acknowledgements

We thank Laura Mameli for secretarial assistance. This work was supported in part by grants from AIRC and from FIRB fund of the Italian Ministry of Instruction, University and Research, Rome.

Author information

Authors and Affiliations

  1. Department of Experimental Oncology, Molecular Targeting Unit, Istituto Nazionale per lo Studio e la Cura dei Tumori, Via Venezian 1, Milano, 20133, Italy

    Serenella M Pupa, Stefania Forti, Patrizia Casalini, Valeria Berno, Piera Aiello, Annamaria Invernizzi & Sylvie Ménard

  2. Department of Cell Biology, Medical University of South Carolina, Charleston, 29425, SC, USA

    Scott W Argraves & Waleed Otwal

  3. General Oncology B Breast Unit, Istituto Nazionale per lo Studio e la Cura dei Tumori, Via Venezian 1, Milano, 20133, Italy

    Roberto Agresti

  4. Department of Experimental Oncology, Human Tumor Immunobiology Unit, Istituto Nazionale per lo Studio e la Cura dei Tumori, Via Venezian 1, Milano, 20133, Italy

    Paola Baldassari, Roberta Mortarini & Andrea Anichini

Authors
  1. Serenella M Pupa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Scott W Argraves
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Stefania Forti
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Patrizia Casalini
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Valeria Berno
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Roberto Agresti
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Piera Aiello
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Annamaria Invernizzi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Paola Baldassari
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Waleed Otwal
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Roberta Mortarini
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Andrea Anichini
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Sylvie Ménard
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sylvie Ménard.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pupa, S., Argraves, S., Forti, S. et al. Immunological and pathobiological roles of fibulin-1 in breast cancer. Oncogene 23, 2153–2160 (2004). https://doi.org/10.1038/sj.onc.1207323

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1207323

Keywords

This article is cited by

Search

Advanced search

Quick links