Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

Modulation of p120E4F transcriptional activity by the Gam1 adenoviral early protein

Oncogene volume 22, pages 2541–2547 (2003)Cite this article

Abstract

Gam1, an early adenoviral CELO protein, is required for viral replication. Consistent with its ability to inhibit histone deacetylation by HDAC1, Gam1 activates transcription. In this report, we identify the cellular transcription factor p120E4F as a Gam1 interaction partner. p120E4F is a low-abundance transcription factor that represses the adenovirus E4 promoter. Here we demonstrate that p120E4F interacts with HDAC1 in vivo and in vitro, and that E4F-mediated transcriptional repression is alleviated by the HDAC inhibitor trichostatin A or by overexpressing Gam1. A mutant E4 promoter unresponsive to E4F-mediated transcriptional repression is also not stimulated by Gam1. Moreover, our cofractionation experiments demonstrate that p120E4F, HDAC1 and Gam1 may be concomitantly present in protein complexes. We conclude that Gam1 activates E4-dependent transcription possibly by inactivating HDAC1.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4

Similar content being viewed by others

References

  • Ahringer J . (2000). Trends Genet., 16, 351–356.

  • Bartl S, Taplick J, Lagger G, Khier H, Kuchler K and Seiser C . (1997). Mol. Cell. Biol., 17, 5033–5043.

  • Chiocca S, Baker A and Cotten M . (1997). J. Virol., 71, 3168–3177.

  • Chiocca S, Kurtev V, Colombo R, Boggio R, Sciurpi MT, Brosch G, Seiser C, Draetta GF and Cotten M . (2002). Curr. Biol., 12, 594–598.

  • Chiocca S, Kurzbauer R, Schaffner G, Baker A, Mautner V and Cotten M . (1996). J. Virol., 70, 2939–2949.

  • Colombo R, Boggio R, Seiser C, Draetta GF and Chiocca S . (2002). EMBO Rep., 3, 1062–1068.

  • Cress WD and Seto E . (2000). J. Cell. Physiol., 184, 1–16.

  • Eckner R, Ewen ME, Newsome D, Gerdes M, DeCaprio JA, Lawrence JB and Livingston DM . (1994). Genes. Dev., 8, 869–884.

  • Fajas L, Paul C, Vie A, Estrach S, Medema R, Blanchard JM, Sardet C and Vignais ML . (2001). Mol. Cell. Biol., 21, 2956–2966.

  • Fajas L, Paul C, Zugasti O, Le Cam L, Polanowska J, Fabbrizio E, Medema R, Vignais ML and Sardet C . (2000). Proc. Natl. Acad. Sci. USA, 97, 7738–7743.

  • Fernandes ER and Rooney RJ . (1997). Mol. Cell. Biol., 17, 1890–1903.

  • Fernandes ER, Zhang JY and Rooney RJ . (1998). Mol. Cell. Biol., 18, 459–467.

  • Fognani C, Della Valle G and Babiss LE . (1993). EMBO J., 12, 4985–4992.

  • Glotzer JB, Saltik M, Chiocca S, Michou AI, Moseley P and Cotten M . (2000). Nature, 407, 207–211.

  • Johnson CA and Turner BM . (1999). Semin. Cell Dev. Biol., 10, 179–188.

  • Kuo MH and Allis CD . (1998). BioEssays, 20, 615–626.

  • Lehrmann H and Cotten M . (1999). J. Virol., 73, 6517–6525.

  • Ng HH and Bird A . (2000). Trends Biochem. Sci., 25, 121–126.

  • Raychaudhuri P, Bagchi S and Nevins JR . (1989). Genes Dev., 3, 620–627.

  • Raychaudhuri P, Rooney R and Nevins JR . (1987). EMBO J., 6, 4073–4081.

  • Rizos H, Diefenbach E, Badhwar P, Woodruff S, Becker TM, Rooney RJ and Kefford RF . (2002). J. Biol. Chem., 21, 21.

  • Rooney RJ . (2001). Cell Growth Differ., 12, 505–516.

  • Rooney RJ, Daniels RR, Jenkins NA, Gilbert DJ, Rothammer K, Morris SW, Higgs DR and Copeland NG . (1998). Mamm. Genome, 9, 320–323.

  • Sandy P, Gostissa M, Fogal V, Cecco LD, Szalay K, Rooney RJ, Schneider C and Del Sal G . (2000). Oncogene, 19, 188–199.

  • Taplick J, Kurtev V, Kroboth K, Posch M, Lechner T and Seiser C . (2001). J. Mol. Biol., 308, 27–38.

  • Yoshida M, Kijima M, Akita M and Beppu T . 1990 J. Biol. Chem 265 17174–17179

Download references

Acknowledgements

We thank Gianni Del Sal for the p120E4F-expressing plasmids. We thank Dr C Seiser for helpful discussions. This work was supported by grants from Associazione Italiana per la Ricerca sul Cancro (AIRC), Consiglio Nazionale delle Ricerche (CNR), Fondazione Italiana per la Ricerca sul Cancro (FIRC) and Telethon.

Author information

Authors and Affiliations

  1. Department of Experimental Oncology, European Institute of Oncology, via Ripamonti, 435, Milan, 20141, Italy

    Riccardo Colombo, Giulio F Draetta & Susanna Chiocca

Authors
  1. Riccardo Colombo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Giulio F Draetta
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Susanna Chiocca
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Susanna Chiocca.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Colombo, R., Draetta, G. & Chiocca, S. Modulation of p120E4F transcriptional activity by the Gam1 adenoviral early protein. Oncogene 22, 2541–2547 (2003). https://doi.org/10.1038/sj.onc.1206379

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1206379

Keywords

This article is cited by

Search

Advanced search

Quick links