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Separation of long-range human TERT gene haplotypes by transformation-associated recombination cloning in yeast

Oncogene volume 22, pages 2452–2456 (2003)Cite this article

Abstract

The hTERT gene encoding a catalytic subunit of human telomerase contains four blocks of variable number of tandem repeats (VNTRs) – two in intron 2 and two in intron 6. The segregation of hTERT VNTRs was analysed in families, revealing that all of them were transmitted through meiosis following a Mendelian inheritance. The work reports a further characterization of the minisatellites in hTERT. We employed transformation-associated recombination (TAR) cloning to isolate parental hTERT alleles and determined the specific combination of minisatellites at each of the polymorphic sites. A long-range haplotyping of hTERT determined by TAR cloning was verified by classical Mendelian analysis. Since such a strategy can be applied for any chromosomal locus, we conclude that recombinational gene capture could greatly facilitate haplotypes analysis.

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Acknowledgements

We thank Miriam Bloom (SciWrite Biomedical Writing & Editing Services) for professional editing. S-H Leem and J-H Kim were partially supported by Grant No. R05-2000-000-00151-0 from the Korea Science & Engineering Foundation.

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Authors and Affiliations

  1. Laboratory of Biosystems and Cancer, National Cancer Institute (NIH), Bethesda, MD, USA

    Jung-Hyun Kim, Sun-Hee Leem & Natalay Kouprina

  2. Department of Biology, Dong-A University, Pusan, 604-714, Korea

    Jung-Hyun Kim, Sun-Hee Leem & Yangil Sunwoo

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  1. Jung-Hyun Kim
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Correspondence to Natalay Kouprina.

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Kim, JH., Leem, SH., Sunwoo, Y. et al. Separation of long-range human TERT gene haplotypes by transformation-associated recombination cloning in yeast. Oncogene 22, 2452–2456 (2003). https://doi.org/10.1038/sj.onc.1206316

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