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Stable and unstable pools of Myc protein exist in human cells

Abstract

The oncoprotein transcription factor Myc plays a crucial role in the control of cell growth and proliferation. Consistent with its potent growth-promoting properties, cells have evolved a number of mechanisms to limit the activity and accumulation of the Myc protein. One of the most striking of these mechanisms is ubiquitin (Ub)-mediated proteolysis, which typically destroys Myc within minutes of its synthesis. Here we show that, despite the extreme instability of the Myc protein, cells contain a pool of Myc that is metabolically stable. Entry of Myc into the stable pool is signaled by an element within the carboxy-terminus of the protein, and is a cell-specific process that is regulated during mitosis and by interaction with Max. These data demonstrate that – even for a rapidly turned-over protein such as Myc – metabolically stable and unstable forms of a protein can co-exist in cells, and suggest that the rate of destruction of Myc molecules is linked to their specific functions.

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References

  • Abu Hatoum O, Gross-Mesilaty S, Breitschopf K, Hoffman A, Gonen H, Ciechanover A, Bengal E . 1998 Mol. Cell. Biol. 18: 5670–5677

  • Blackwood EM, Eisenman RN . 1991 Science 251: 1211–1217

  • Carrano AC, Eytan E, Hershko A, Pagano M . 1999 Nat. Cell Biol. 1: 193–199

  • Ciechanover A, DiGiuseppe JA, Bercovich B, Orian A, Richter JD, Schwartz AL, Brodeur GM . 1991 Proc. Natl. Acad. Sci. USA 88: 139–143

  • Evan GI, Hancock DC . 1985 Cell 43: 253–261

  • Furstenthal L, Swanson C, Kaiser BK, Eldridge AG, Jackson PK . 2001 Nat. Cell Biol. 3: 715–722

  • Gregory MA, Hann SR . 2000 Mol. Cell. Biol. 20: 2423–2435

  • Hancock R . 2000 Chromosoma 109: 219–225

  • Hann SR, Eisenman RN . 1984 Mol. Cell. Biol. 4: 2486–2497

  • Henriksson M, Luscher B . 1996 Adv. Cancer Res. 68: 109–182

  • Land H, Parada LF, Weinberg RA . 1983 Nature 304: 596–602

  • Mendez J, Stillman B . 2000 Mol. Cell. Biol. 20: 8602–8612

  • Mendez J, Zou-Yang XH, Kim SY, Hidaka M, Tansey WP, Stillman B . 2002 Mol. Cell 9: 481–491

  • Molinari E, Gilman M, Natesan S . 1999 EMBO J. 18: 6439–6447

  • Niklinski J, Claassen G, Meyers C, Gregory MA, Allegra CJ, Kaye FJ, Hann SR, Zajac-Kaye M . 2000 Mol. Cell. Biol. 20: 5276–5284

  • Peukert K, Staller P, Schneider A, Carmichael G, Hanel F, Eilers M . 1997 EMBO J. 16: 5672–5686

  • Prescott DM, Bender MA . 1962 Exp. Cell Res. 26: 260–268

  • Rudolph C, Adam G, Simm A . 1999 Anal. Biochem. 269: 66–71

  • Sacco-Bubulya P, Spector DL . 2002 J. Cell Biol. 156: 425–436

  • Salghetti SE, Caudy AA, Chenoweth JG, Tansey WP . 2001 Science 293: 1651–1653

  • Salghetti SE, Kim SY, Tansey WP . 1999 EMBO J. 18: 717–726

  • Salghetti SE, Muratani M, Wijnen H, Futcher B, Tansey WP . 2000 Proc. Natl. Acad. Sci. USA 97: 3118–3123

  • Sears R, Nuckolls F, Haura E, Taya Y, Tamai K, Nevins JR . 2000 Genes Dev. 14: 2501–2514

  • Treier M, Staszewski LM, Bohmann D . 1994 Cell 78: 787–798

  • Varshavsky A . 1997 Trends Biochem. Sci. 22: 383–387

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Acknowledgements

For reagents we thank C Bautista, D Bohmann, M Eilers, W Herr, N Hernandez and R Whittaker. We thank A Herbst, S Kim, Y Lazebnik, M Muratani and S Muthuswamy for discussions and critical comments on the manuscript. WP Tansey is a Leukemia and Lymphoma Society of America Scholar. KA Tworkowski is supported by training grant NIGMS-NIH 1T32-GM08468. This work was supported by the CSHL Cancer Center Support Grant CA45508 and by US Public Health Service grant CA-13106 from the National Cancer Institute.

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Correspondence to William P Tansey.

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Tworkowski, K., Salghetti, S. & Tansey, W. Stable and unstable pools of Myc protein exist in human cells. Oncogene 21, 8515–8520 (2002). https://doi.org/10.1038/sj.onc.1205976

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Keywords

  • Myc
  • Max
  • ubiquitin-mediated proteolysis

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