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pRb2/p130 promotes radiation-induced cell death in the glioblastoma cell line HJC12 by p73 upregulation and Bcl-2 downregulation

Oncogene volume 21, pages 5897–5905 (2002)Cite this article

Abstract

This study shows that in the glioblastoma hamster cell line HJC12 the retinoblastoma family member pRb2/p130 enhances γ-radiation-induced cell death. In HJC12 cells the tetracycline-regulated expression of pRb2/p130 increased the percentage of γ-radiation-induced apoptotic cells from 27 to 47%. pRb2/p130 overexpression was associated with the downregulation of the anti-apoptotic factor Bcl-2 and the upregulation of the steady-state protein levels of the pro-apoptotic transcription factor p73. In particular, RT–PCR showed a significant increase in the expression of the p73δ isoform when pRb2/p130 was overexpressed. The ability of pRb2/p130 to modulate apoptosis was not associated with its role in mediating G0/G1 arrest during cell cycle progression. Our data suggest a role for pRb2/p130 in glioblastoma γ-radiation-induced cell death, indicating that the antitumoral action of pRb2/p130 can regulate both inhibition of cell cycle progression and induction of cell death.

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Acknowledgements

We especially thank Mr Charles Zegar for his support of our research efforts. This work was supported in part by National Institute of Health Grant RO1 CA60999/01A1, by Grant PO1 NS36466 (to A Giordano and K Khalili), by Grant PO1-CA56309 (to A Giordano), by the Sbarro Health Research Organization, by research grant Min. Sanita', Telethon E872, AIRC, MURST-Corn 98, EU grant OLG1-1999-00739 (to G Melino). V Masciullo was supported by a training grant from the National Cancer Institute (PHS 5 T32 CA09137). PP Claudio is the recipient of a fellowship from the ‘Associazione Leonardo di Capua’.

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Authors and Affiliations

  1. Department of Pathology, Anatomy and Cell Biology, Jefferson Medical College, Philadelphia, 19107, Pennsylvania, PA, USA

    Bruna Pucci, Valeria Masciullo & Antonio Giordano

  2. Sbarro Institute for Cancer Research and Molecular Medicine, College of Science and Technology, Temple University, Philadelphia, 19122, Pennsylvania, PA, USA

    Pier Paolo Claudio & Antonio Giordano

  3. Dipartimento di Scienze Odontostomatologiche e Maxillo-facciali, Universita' di Napoli ‘Federico II’, Naples, 80131, Italy

    Pier Paolo Claudio

  4. Dipartimento di Medicina Interna Universita' di Roma ‘Tor Vergata’, Rome, 00133, Italy

    Lorenza Bellincampi

  5. Istituto Dermopatico dell'Immacolata (IDI-IRCCS) c/o Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Laboratorio di Biochimica, Universita' di Roma ‘Tor Vergata’, Rome, 00133, Italy

    Alessandro Terrinoni & Gerry Melino

  6. Center for Neuro Virology and Cancer Biology, Temple University, Philadelphia, 19122, Pennsylvania, PA, USA

    Kamel Khalili

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Correspondence to Antonio Giordano.

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Pucci, B., Claudio, P., Masciullo, V. et al. pRb2/p130 promotes radiation-induced cell death in the glioblastoma cell line HJC12 by p73 upregulation and Bcl-2 downregulation. Oncogene 21, 5897–5905 (2002). https://doi.org/10.1038/sj.onc.1205750

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