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  • Oncogenomics
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Cloning and characterization of a novel 90 kDa ‘companion’ auto-antigen of p62 overexpressed in cancer

Oncogene volume 21, pages 5006–5015 (2002)Cite this article

Abstract

Recently our laboratory identified a cytoplasmic RNA-binding protein p62 which binds to and regulates the expression of IGF II mRNA. p62 was initially shown to be recognized by auto-antibodies in hepatocellular carcinoma (HCC) but now anti-p62 has been described in diverse malignancies. p62 is uniformly expressed in fetal liver and prominently in 33% of HCC nodules, but not detectable in adult liver or normal tissue adjacent to HCC nodules. In this study, a 90 kDa protein (p90), auto-antibodies to which were found associated with anti-p62 responses in the same HCC patient group, was identified by cDNA expression cloning. Indirect immunofluorescence showed that, like p62, p90 localized to the cytoplasm in cultured cells and mouse fetal, but not adult liver. Among 11 human gastric cancer tissues examined, p90 was overexpressed in six (55%). Together with other cancer associated auto-antibodies such as anti-p53, anti-p62, anti-Koc, and anti-CENP-F, auto-antibodies to p90 represent a new marker for tumors such as HCC and gastric cancer. Our data support the working hypothesis that auto-antibody production in cancer may be directly linked to aberrant auto-antigen expression.

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Abbreviations

GC:

gastric cancer

HCC:

hepatocellular carcinoma

IMP:

IGF-II mRNA binding protein

IP:

immunoprecipitation

TnT:

in vitro transcription and translation

References

  • Altschul SF, Madden TL, Schaffer AA, Zhang Z, Miller W, Lipman DJ . 1997 Nucleic Acids Res. 25: 3389–3402

  • Braverman R, Bhattacharya B, Feuerstein N, Cooper HL . 1986 J. Biol. Chem. 261: 14342–14348

  • Bressac B, Kew M, Wands J, Ozturk M . 1991 Nature 350: 429–431

  • Chan EKL, Buyon JP . 1994 Manual of Biological Markers of Disease van Venrooij WJ and Maini RN (eds) Kluwer Academic Publishers: Dordrecht, Netherlands pp. 1–18

    Google Scholar 

  • Chan EKL, Fritzler MJ . 1998 Elec. J. Biotechnol. 1: 1–10

  • Chan EKL, Hamel JC, Buyon JP, Tan EM . 1991 J. Clin. Invest. 87: 68–76

  • Covini G, von Mühlen CA, Pacchetti S, Colombo M, Chan EKL, Tan EM . 1997 J. Hepatol. 26: 1255–1265

  • Deshler JO, Highett MI, Abramson T, Schnapp BJ . 1998 Curr. Biol. 8: 489–496

  • Doyle GA, Betz NA, Leeds PF, Fleisig AJ, Prokipcak RD, Ross J . 1998 Nucleic Acids Res. 26: 5036–5044

  • Fields S, Song O . 1989 Nature 340: 245–246

  • Fritzler MJ, Kerfoot SM, Feasby TE, Zochodne DW, Westendorf JM, Dalmau JO, Chan EKL . 2000 J. Investig. Med. 48: 28–39

  • Hardin JA . 1986 Arthritis Rheum. 29: 457–460

  • Havin L, Git A, Elisha Z, Oberman F, Yaniv K, Schwartz SP, Standart N, Yisraeli JK . 1998 Genes Dev. 12: 1593–1598

  • Imai H, Nakano Y, Kiyosawa K, Tan EM . 1993 Cancer 71: 26–35

  • Kikuno R, Nagase T, Suyama M, Waki M, Hirosawa M, Ohara O . 2000 Nucleic Acids Res. 28: 331–332

  • Kiyosawa K, Sodeyama T, Tanaka E, Gibo Y, Yoshizawa K, Nakano Y, Furuta S, Akahane Y, Nishioka K, Purcell RH . 1990 Hepatology 12: 671–675

  • Kozak M . 1987 Nucleic Acids Res. 15: 8125–8148

  • Lu M, Nakamura RM, Dent ED, Zhang JY, Nielsen FC, Christiansen J, Chan EKL, Tan EM . 2001 Am. J. Pathol. 159: 945–953

  • Lupas A . 1997 Curr. Opin. Struct. Biol. 7: 388–393

  • Mueller-Pillasch F, Lacher U, Wallrapp C, Micha A, Zimmerhackl F, Hameister H, Varga G, Friess H, Buchler M, Beger HG, Vila MR, Adler G, Gress TM . 1997 Oncogene 14: 2729–2733

  • Nagase T, Kikuno R, Ishikawa K, Hirosawa M, Ohara O . 2000 DNA Res. 7: 143–150

  • Nielsen J, Christiansen J, Lykke-Andersen J, Johnsen AH, Wewer UM, Nielsen FC . 1999 Mol. Cell. Biol. 19: 1262–1270

  • Ochs RL, Stein Jr TW, Chan EKL, Ruutu M, Tan EM . 1996 Mol. Biol. Cell 7: 1015–1024

  • Rattner JB, Rees J, Whitehead CM, Casiano CA, Tan EM, Humbel RL, Conrad K, Fritzler MJ . 1997 Clin. Invest. Med. 20: 308–319

  • Ross AF, Oleynikov Y, Kislauskis EH, Taneja KL, Singer RH . 1997 Mol. Cell. Biol. 17: 2158–2165

  • Selak S, Chan EKL, Schoenroth L, Senecal JL, Fritzler MJ . 1999 J. Investig. Med. 47: 311–318

  • Shaw G, Kamen R . 1986 Cell 46: 659–667

  • Simonetti RG, Camma C, Fiorello F, Politi F, D'Amico G, Pagliaro L . 1991 Dig. Dis. Sci. 36: 962–972

  • Soussi T . 2000 Cancer Res. 60: 1777–1788

  • Tan EM . 1989 Adv. Immunol. 44: 93–151

  • Tan EM . 2001 J. Clin. Invest. 108: 1411–1415

  • Tan EM, Chan EKL, Sullivan KF, Rubin RL . 1988 Clin. Immunol. Immunopathol. 47: 121–141

  • Wang D, Buyon JP, Zhu W, Chan EKL . 1999 J. Clin. Invest. 104: 1265–1275

  • Wang D, Chan EKL . 1996 J. Invest. Dermatol. 107: 610–614

  • Zhang JY, Chan EKL, Peng XX, Lu M, Wang X, Mueller F, Tan EM . 2001a Clin. Immunol. 100: 149–156

  • Zhang JY, Chan EKL, Peng XX, Tan EM . 1999 J. Exp. Med. 189: 1101–1110

  • Zhang JY, Dai M, Wang X, Lu WQ, Li DS, Zhang MX, Wang KJ, Dai LP, Han SG, Zhou YF, Zhuang H . 1998a Int. J. Epidemiol. 27: 574–578

  • Zhang JY, Wang X, Han SG, Zhuang H . 1998b Am. J. Trop. Med. Hyg. 59: 947–951

  • Zhang JY, Zhu W, Imai H, Kiyosawa K, Chan EKL, Tan EM . 2001b Clin. Exp. Immunol. 125: 3–9

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Acknowledgements

We thank Dr Eng M Tan for his encouragement, support and comments during this study. This is publication 14396-MEM from The Scripps Research Institute. This work was supported in part by National Institutes of Health Grants CA56956 and AI39645, the Sam and Rose Stein Charitable Trust, and NIH grant M01RR00833 provided to the General Clinical Research Center of The Scripps Research Institute.

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  1. Jianying Y Zhang

    Present address: Department of Biological Sciences, University of Texas at El Paso, 500 West University Avenue, El Paso, 79968-0519, TX

Authors and Affiliations

  1. Department of Molecular and Experimental Medicine, WM Keck Autoimmune Disease Center and DNA Core Laboratory for Structural Analysis, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, 92037, California, CA, USA

    Linda Soo Hoo, Jianying Y Zhang & Edward KL Chan

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  1. Linda Soo Hoo
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Correspondence to Edward KL Chan.

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Hoo, L., Zhang, J. & Chan, E. Cloning and characterization of a novel 90 kDa ‘companion’ auto-antigen of p62 overexpressed in cancer. Oncogene 21, 5006–5015 (2002). https://doi.org/10.1038/sj.onc.1205625

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