Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

Identification of genes associated with head and neck carcinogenesis by cDNA microarray comparison between matched primary normal epithelial and squamous carcinoma cells

Oncogene volume 21, pages 2634–2640 (2002)Cite this article

Abstract

In order to identify genes involved in head and neck carcinogenesis, we compared the gene expression profile in matched primary normal epithelial cells and primary head and neck cancer cells from the same patients. A cDNA microarray analysis consisting of 12 530 human genes revealed significant changes in the expression of 213 genes, with 91 genes being up-regulated and 122 being down-regulated. This comprehensive list of genes includes those associated with signal transduction (growth factors), cell structure, cell cycle, transcription, apoptosis, and cell–cell adhesion. Further analysis of nine genes involved in cell–cell interaction, using Western blot and/or reverse transcription (RT)–PCR of four paired cell lines supported the reliability of our microarray analysis. More specifically, our study provides the first evidence that claudin-7 and connexin 31.1 are down-regulated in head and neck squamous cell carcinomas (HNSCC) compared to normal cells. These findings provide a large body of information regarding gene expression profiles associated with head and neck carcinogenesis, and also represent a source of potential targets for HNSCC prevention and/or therapeutics.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3

Similar content being viewed by others

References

  • Al Moustafa A-E, Benlimame N, Yen L, Alaoui-Jamali MA . 2002 Lung Cancer in press

  • Anttonen A, Kajanti M, Heikkila P, Jalkanen M, Joensuu H . 1999 Br. J. Cancer 79: 558–564

  • Bagutti C, Speight PM, Watt FM . 1998 J. Pathol. 186: 8–16

  • Bentz BG, Haines III GK, Radosvich JA . 2000 Laryngoscope 110: 1642–1647

  • Beyer EC, Paul DL, Goodenough DA . 1990 J. Memb. Biol. 3: 187–194

  • Bishop AJ, Schiestl RH . 2001 Biochim. Biophys. Acta. 1471: M109–M121

  • Brissette JL, Kumar NM, Gilula NB, Hall JE, Dotto GP . 1994 Proc. Natl. Acad. Sci. USA 91: 6453–6457

  • Budunova IV, Carbajal S, Slaga TJ . 1995 Carinogenesis 11: 2717–2724

  • Chen Z, Malhotra PS, Thomas GR, Ondrey FG, Duffey DC, Smith CW, Enamorado I, Yeh NT, Kroog GS, Rudy S, McCullagh L, Mousa S, Quezado M, Herscher LL, Van Waes C . 1999 Clin. Cancer Res. 5: 1369–1379

  • Chu YW, Seftor EA, Romer LH, Hendrix MJ . 1996 Am. J. Pathol. 148: 63–69

  • Clavel CE, Nollet F, Berx G, Tejpar S, Nawrocki-Raby B, Kaplan HH, van Roy FM, Birembaut PL . 2001 J. Pathol. 194: 20–26

  • Duffey DC, Crowl-Bancroft CV, Chen Z, Ondrey FG, Nejad-Sattari M, Dong G, Van Waes C . 2000 Br. J. Cancer 83: 1367–1374

  • Gofuku J, Shiozaki H, Tsujinaka T, Inoue M, Tamura S, Doki Y, Matsui S, Tsukita S, Kikkawa N, Monden M . 1999 Am. J. Clin. Pathol. 111: 29–37

  • Grunwald GB . 1993 Curr. Opin. Cell Biol. 5: 797–805

  • Hedenfalk I, Duggan D, Chen Y, Radmacher M, Bittner M, Simon R, Meltzer P, Gusterson B, Esteller M, Kallioniemi OP, Wilfond B, Borg A, Trent J . 2001 N. Engl. J. Med. 344: 539–548

  • Heiskala M, Peterson PA, Yang Y . 2001 Traffic 2: 93–98

  • Jefferies S, Foulkes WD . 2001 Oral Oncol. 37: 115–126

  • Kasid U, Pfeifer A, Brennan T, Beckett M, Weichselbaum RR, Dritschilo A, Mark GE . 1989 Science 243: 1354–1356

  • Leethanakul C, Patel V, Gillespie J, Pallente M, Ensley JF, Koontongkaew S, Liotta LA, Emmert-Buck M, Gutkind JS . 2000 Oncogene 19: 3220–3224

  • Li Z, Gallin WJ, Lauzon G, Pasdar M . 1998 J. Cell Sci. 111: 1005–1019

  • Li G, Herlyn M . 2000 Mol. Med. Today 6: 163–169

  • Maniotis AJ, Folberg R, Hess A, Seftor EA, Gardner LM, Pe'er J, Trent JM, Meltzer PS, Hendrix MJ . 1999 Am. J. Pathol. 155: 739–752

  • Miller AC, Kariko K, Myers CE, Clark EP, Samid D . 1993 Int. J. Cancer 53: 302–307

  • Miura K, Suzuki S, Tanita J, Shinkawa H, Satoh K, Tsuchida S . 1997 Jpn. J. Cancer Res. 88: 143–151

  • Morifuji M, Taniguchi S, Sakai H, Nakabeppu Y, Ohishi M . 2000 Am. J. Pathol. 156: 1317–1326

  • Ninomiya I, Endo Y, Fushida S, Sasagawa T, Miyashita T, Fujimura T, Nishimura G, Tani T, Hashimoto T, Yagi M, Shimizu K, Ohta T, Yonemura Y, Inoue M, Sasaki T, Miwa K . 2000 Int. J. Cancer 85: 757–761

  • Ono K, Tanaka T, Tsunoda T, Kitahara O, Kihara C, Okamoto A, Ochiai K, Takagi T, Nakamura Y . 2000 Cancer Res. 60: 5007–5011

  • Ozawa M, Baribault H, Kemler R . 1989 EMBO J. 8: 1711–1717

  • Ozawa M, Kemler R . 1992 J. Cell Biol. 116: 989–996

  • Paterson IC, Matthews JB, Huntley S, Robinson CM, Fahey M, Parkinson EK, Prime SS . 2001 J. Pathol. 193: 458–467

  • Pukkila MJ, Virtaniemi JA, Kumpulainen EJ, Pirinen RT, Johansson RT, Valtonen HJ, Juhola MT, Kosma VM . 2001 J. Clin. Pathol. 54: 42–47

  • Reid BC, Winn DM, Morse DE, Pendrys DG . 2000 Oral Oncol. 36: 414–420

  • Shapiro L, Fannon AM, Kwong PD, Thompson A, Lehmann MS, Grubel G, Legrand JF, Als-Nielsen J, Colman DR, Hendrickson WA . 1995 Nature 374: 327–337

  • Takeichi M . 1990 Annu. Rev. Biochem. 59: 237–252

  • Tomson AM, Scholma JA, Meijer B, Koning JG, de Jong KM, van der Werf M . 1996 Clin. Exp. Metast. 14: 501–511

  • Villaret DB, Wang T, Dillon D, Xu J, Sivam D, Cheever MA, Reed SG . 2000 Laryngoscope 110: 374–381

  • Vokes EE, Weichselbaum RR, Lippman SM, Hong WK . 1993 N. Engl. J. Med. 328: 184–194

  • Wang T, Hopkins D, Schmidt C, Silva S, Houghton R, Takita H, Repasky E, Reed SG . 2000 Oncogene 19: 1519–1528

  • Witt CJ, Gabel SP, Meisinger J, Werra G, Liu SW, Young MR . 2000 Otolaryngol Head Neck Surg. 122: 721–727

  • Wong DT, Todd R, Tsuji T, Donoff RB . 1996 Crit. Rev. Oral Biol. Med. 7: 319–328

  • Yamasaki H, Omori Y, Zaidan-Dagli ML, Mironov N, Mesnil M, Krutovskikh V . 1999 Cancer Detect. Prev. 4: 273–279

  • Yang Y, Hao J, Liu X, Dalkin B, Nagle RB . 1997 Am. J. Pathol 150: 693–704

  • Yokoyama K, Kamata N, Hayashi E, Hoteiya T, Ueda N, Fujimoto R, Nagayama M . 2001 Oral Oncol. 37: 65–71

Download references

Acknowledgements

We are grateful to L Yen, PJ Scrivens and Drs D Hamilton, N Bachnou and T Hudson for critical reading of the manuscript. We thank Dr L Rochon for reviewing the histopathological slides of the cases described herein and for helpful discussions. We also thank M Tong and N Hamel for their technical assistance. R Sladek is the recipient of a fellowship from the Canadian Institutes for Health Research. This work supported by the National Cancer Institute of Canada grant # 010254. WD Foulkes is a Chercheur Clinicien Boursier of the Fonds de la Recherche en Santé du Québec.

Author information

Authors and Affiliations

  1. Departments of Medicine, Lady Davis Institute for Medical Research of the Sir Mortimer B Davis-Jewish General Hospital, Oncology and Pathology, and McGill Center for Translational Research in Cancer, 3755, Ch. de la Cote Ste-Catherine, Montreal, H3T 1E2, Quebec, Canada

    Ala-Eddin Al Moustafa, Moulay A Alaoui-Jamali, Gerald Batist, Maite Hernandez-Perez, Corinne Serruya, Lesley Alpert & William D Foulkes

  2. Head and Neck Cancer Division, Department of Surgery, Sir MB Davis-Jewish General Hospital, Montreal, Quebec, Canada

    Martin J Black

  3. Research Institute of McGill University Health Center, Montreal, Quebec, Canada

    Robert Sladek & William D Foulkes

  4. Montreal Genome Centre, McGill University, Montreal, Quebec, Canada

    Robert Sladek

  5. Departments of Human Genetics and Oncology, Program in Cancer Genetics, McGill University, Montreal, Quebec, Canada

    William D Foulkes

Authors
  1. Ala-Eddin Al Moustafa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Moulay A Alaoui-Jamali
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gerald Batist
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maite Hernandez-Perez
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Corinne Serruya
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lesley Alpert
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Martin J Black
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Robert Sladek
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. William D Foulkes
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Moulay A Alaoui-Jamali.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Al Moustafa, AE., Alaoui-Jamali, M., Batist, G. et al. Identification of genes associated with head and neck carcinogenesis by cDNA microarray comparison between matched primary normal epithelial and squamous carcinoma cells. Oncogene 21, 2634–2640 (2002). https://doi.org/10.1038/sj.onc.1205351

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1205351

Keywords

This article is cited by

Search

Advanced search

Quick links