Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

The human papillomavirus type 16 (HPV-16) E5 protein sensitizes human keratinocytes to apoptosis induced by osmotic stress

Oncogene volume 21, pages 947–953 (2002)Cite this article

Abstract

We have studied the role of the HPV-16 E5 protein in apoptosis, using HaCaT cell lines stably transfected with either E5 (HaCaT/E5) or the empty vector (HaCaT/pMSG) as control. When subjected to a hyperosmolar concentration of sorbitol, HaCaT/E5 cells respond with cytochrome c release, activation of caspase-3, -8, and -9, and PARP-cleavage, showing that the mitochondria and death-receptor mediated apoptotic pathways are involved in subsequent cell death. Similar effects are observed for the control cells only after extended sorbitol treatment. Thus, E5-expressing cells are more sensitive to osmotic stress, perhaps because of modifications of the cellular membranes caused by this strongly hydrophobic molecule.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8

Similar content being viewed by others

References

  • Aragane Y, Kulms D, Metze D, Wilkes G, Pöppelmann B, Luger TA, Schwarz T . 1998 J. Cell Biol. 140: 171–182

  • Assefa Z, Vantieghem A, Garmyn M, Declercq W, Vandenabeele P, Vandenheede JR, Bouillon R, Merlevede W, Agostinis P . 2000 J. Biol. Chem. 275: 21416–21421

  • Boukamp P, Petrussevska RT, Breitkreutz D, Hornung J, Markham A, Fusenig NE . 1988 J. Cell Biol. 106: 761–771

  • Budihardjo I, Oliver H, Lutter M, Luo X, Wang X . 1999 Annu. Rev. Cell Dev. Biol. 15: 269–290

  • Conrad M, Bubb VJ, Schlegel R . 1993 J. Virol. 67: 6170–6178

  • Crusius K, Auvinen E, Steuer B, Gaissert H, Alonso A . 1998 Exp. Cell Res. 241: 76–83

  • Crusius K, Kaszkin M, Kinzel V, Alonso A . 1999 Oncogene 18: 6714–6718

  • Crusius K, Rodriguez I, Alonso A . 2000 Virus Genes 20: 61–65

  • Earnshaw WC, Martins LM, Kaufmann SH . 1999 Annu. Rev. Biochem. 68: 383–424

  • Faulkner-Valle G, Banks L . 1995 J. Gen. Virol. 76: 1239–1245

  • Fesik SW . 2000 Cell 103: 273–282

  • Hachisuka H, Nomura H, Mori O, Nakano S, Okubo K, Kusuhara M, Karashima M, Tanikawa E, Higuchi M, Sasai Y . 1990 Cell Tissue Res. 260: 207–210

  • Hengartner MO . 2000 Nature 407: 770–776

  • Jost M, Gasparro FP, Jensen PJ, Rodeck U . 2001 J. Invest. Dermatol. 116: 860–866

  • Kaufmann SH, Desnoyers S, Ottaviano Y, Davidson NE, Poirier GG . 1993 Cancer Res. 53: 3976–3985

  • Kell B, Jewers RJ, Cason J, Pakarian F, Kaye JN, Best JM . 1994 J. Gen. Virol. 75: 2451–2456

  • Lang F, Busch GL, Ritter M, Völkl H, Waldegger S, Gulbins E, Häussinger D . 1998 Physiol. Rev. 78: 247–306

  • Leechanachai P, Banks L, Moreau F, Matlashewski G . 1992 Oncogene 7: 19–25

  • Leptak C, Ramon y Cajal S, Kulke R, Horwitz BH, Riese II DJ, Dotto GP, DiMaio D . 1991 J. Virol. 65: 7078–7083 [Correction: J. Virol. 66 1833]

  • Oelze I, Kartenbeck J, Crusius K, Alonso A . 1995 J. Virol. 69: 4489–4494

  • Pim D, Collins M, Banks L . 1992 Oncogene 7: 27–32

  • Rich T, Allen RL, Wyllie AH . 2000 Nature 407: 777–783

  • Scaffidi C, Medema JP, Krammer PH, Peter ME . 1997 J. Biol. Chem. 272: 26953–26958

  • Shimizu H, Banno Y, Sumi N, Naganawa T, Kitajima Y, Nozawa Y . 1999 J. Invest. Dermatol. 112: 769–774

  • Stoler MH, Rhodes CR, Whitbeck A, Wolinsky SM, Chow LT, Broker TR . 1992 Hum. Pathol. 23: 117–128

  • Straight SW, Hinkle PM, Jewers RJ, McCance DJ . 1993 J. Virol. 67: 4521–4532

  • Strasser A, O'Connor L, Dixit VM . 2000 Annu. Rev. Biochem. 69: 217–245

  • Thompson CB . 1995 Science 267: 1456–1462

  • Ullman CG, Haris PI, Kell B, Cason J, Jewers RJ, Best JM, Emery VC, Perkins SJ . 1994 Biochem. Soc. Trans. 22: 439–

  • Wang J, Lenardo MJ . 2000 J. Cell Science 113: 753–757

  • Zur Hausen H . 1991a Virology 184: 9–13

  • Zur Hausen H . 1991b Science 254: 1167–1173

Download references

Acknowledgements

We thank Ulrike Rothermel for excellent technical assistance and John Wray for critical reading of the manuscript. We are grateful to Pascal Tomakidi and Wolfgang Kabsch for valuable discussions.

Author information

Authors and Affiliations

  1. Deutsches Krebsforschungszentrum, Im Neuenheimer Feld-242, 69120 Heidelberg, Germany

    Kirsten Kabsch & Angel Alonso

Authors
  1. Kirsten Kabsch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Angel Alonso
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kirsten Kabsch.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kabsch, K., Alonso, A. The human papillomavirus type 16 (HPV-16) E5 protein sensitizes human keratinocytes to apoptosis induced by osmotic stress. Oncogene 21, 947–953 (2002). https://doi.org/10.1038/sj.onc.1205147

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1205147

Keywords

This article is cited by

Search

Advanced search

Quick links