Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

G2 phase cell cycle arrest in human skin following UV irradiation

Oncogene volume 20, pages 6103–6110 (2001)Cite this article

Abstract

The contribution of the short wavelength ultraviolet (UV) component of sunlight to the aetiology of skin cancer has been widely acknowledged, although its direct contribution to tumour initiation or progression is still poorly understood. The loss of normal cell cycle controls, particularly checkpoint controls, are a common feature of cancer. UV radiation causes both G1 and G2 phase checkpoint arrest in vitro cultured cells. In this study we have investigated the cell cycle responses to suberythemal doses of UV on skin. We have utilized short-term whole organ skin cultures, and multi parameter immunohistochemical and biochemical analysis to demonstrate that basal and suprabasal layer melanocytes and keratinocytes undergo a G2 phase cell cycle arrest for up to 48 h following irradiation. The arrest is associated with increased p16 expression but no apparent p53 involvement. This type of organ culture provides a very useful model system, combining the ease of in vitro manipulation with the ability to perform detailed molecular analysis in a normal tissue environment.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8

Similar content being viewed by others

References

  • Campbell C, Quinn AG, Angus B, Farr PM, Rees JL . 1993 Cancer Res. 53: 2697–2699

  • Castellano M, Parmiani G . 1999 Melanoma Res. 9: 421–432

  • Chakraborty A, Pawelek J . 1993 J. Cell. Physiol. 157: 344–350

  • Chakraborty AK, Funasaka Y, Slominski A, Bolognia J, Sodi S, Ichihashi M, Pawelek JM . 1999 Ann. N.Y. Acad. Sci. 885: 100–116

  • Cliby WA, Roberts CJ, Cimprich KA, Stringer CM, Lamb JR, Schreiber SL, Friend SH . 1998 EMBO J. 17: 159–169

  • Dickson MA, Hahn WC, Ino Y, Ronfard V, Wu JY, Weinberg RA, Louis DN, Li FP, Rheinwald JG . 2000 Mol. Cell. Biol. 20: 1436–1447

  • Elder DE . 1989 J. Invest. Dermatol. 92: 297S–303S

  • Freeman SE, Gange RW, Sutherland JC, Matzinger EA, Sutherland BM . 1987 J. Invest. Dermatol. 88: 430–433

  • Friedmann PS, Gilchrest BA . 1987 J. Cell. Physiol. 133: 88–94

  • Gabrielli BG, Clark JM, McCormack AK, Ellem KA . 1997a J. Biol. Chem. 272: 28607–28614

  • Gabrielli BG, Clark JM, McCormack AK, Ellem KA . 1997b Oncogene 15: 749–758

  • Gabrielli BG, De Souza CP, Tonks ID, Clark JM, Hayward NK, Ellem KA . 1996 J. Cell Sci. 109: 1081–1093

  • Gabrielli BG, Sarcevic B, Sinnamon J, Walker G, Castellano M, Wang XQ, Ellem KA . 1999 J. Biol. Chem. 274: 13961–13969

  • Haddad MM, Xu W, Schwahn DJ, Liao F, Medrano EE . 1999 Exp. Cell Res. 253: 561–572

  • Herzinger T, Funk JO, Hillmer K, Eick D, Wolf DA, Kind P . 1995 Oncogene 11: 2151–2156

  • Honigsmann H, Brenner W, Tanew A, Ortel B . 1987 J. Photochem. Photobiol. B 1: 33–43

  • Huang LC, Clarkin KC, Wahl GM . 1996 Proc. Natl. Acad. Sci. USA 93: 4827–4832

  • Im S, Moro O, Peng F, Medrano EE, Cornelius J, Babcock G, Nordlund JJ, Abdel-Malek ZA . 1998 Cancer Res. 58: 47–54

  • Iyengar B . 1994 Melanoma Res. 4: 293–295

  • Ley KD, Ellem KA . 1992 Carcinogenesis 13: 183–187

  • Libow LF, Scheide S, DeLeo VA . 1988 Pigment Cell Res. 1: 397–401

  • Lu X, Lane DP . 1993 Cell 75: 765–778

  • McLane JA, Pawelek JM . 1988 Biochemistry 27: 3743–3747

  • Medrano EE, Im S, Yang F, Abdel-Malek ZA . 1995 Cancer Res. 55: 4047–4052

  • Milligan A, Gabrielli BG, Clark JM, Hayward NK, Ellem KA . 1998 Mutat. Res. 422: 43–53

  • Pavey S, Conroy S, Russell T, Gabrielli B . 1999 Cancer Res. 59: 4185–4189

  • Petrocelli T, Poon R, Drucker DJ, Slingerland JM, Rosen CF . 1996 Oncogene 12: 1387–1396

  • Quevedo Jr WC . 1969 Am. Zool. 9: 531–540

  • Ruas M, Peters G . 1998 Biochim. Biophys. Acta 1378: F115–F177

  • Sarkaria JN, Busby EC, Tibbetts RS, Roos P, Taya Y, Karnitz LM, Abraham RT . 1999 Cancer Res. 59: 4375–4382

  • Shapiro GI, Edwards CD, Ewen ME, Rollins BJ . 1998 Mol. Cell. Biol. 18: 378–387

  • Soufir N, Moles JP, Vilmer C, Moch C, Verola O, Rivet J, Tesniere A, Dubertret L, Basset-Seguin N . 1999 Oncogene 18: 5477–5481

  • Tada A, Suzuki I, Im S, Davis MB, Cornelius J, Babcock G, Nordlund JJ, Abdel-Malek ZA . 1998 Cell Growth Differ. 9: 575–584

  • Tam SW, Shay JW, Pagano M . 1994 Cancer Res. 54: 5816–5820

  • Thody AJ, Graham A . 1998 Pigment Cell Res. 11: 265–274

  • Wang XQ, Gabrielli BG, Milligan A, Dickinson JL, Antalis TM, Ellem KA . 1996 Cancer Res. 56: 2510–2514

  • Watters D, Garrone B, Coomer J, Johnson WE, Brown G, Parsons P . 1998 Biochem. Pharmacol. 55: 1691–1699

  • Wikonkal NM, Brash DE . 1999 J. Investig. Dermatol. Symp. Proc. 4: 6–10

  • Zhan Q, Antinore MJ, Wang XW, Carrier F, Smith ML, Harris CC, Fornace Jr AJ . 1999 Oncogene 18: 2892–2900

  • Ziegler A, Jonason AS, Leffell DJ, Simon JA, Sharma HW, Kimmelman J, Remington L, Jacks T, Brash DE . 1994 Nature 372: 773–776

Download references

Acknowledgements

We would like to thank Dr Nicholas Hayward for his continued support and Drs Hayward and Nick Saunders for critical reading of the manuscript. This project was funded by the National Health and Medical Research Council of Australia. B Gabrielli was supported by an Australian Research Council Fellowship.

Author information

Authors and Affiliations

  1. Department of Pathology, Joint Experimental Oncology Program, University of Queensland, Brisbane, Australia

    Sandra Pavey, Terry Russell & Brian Gabrielli

Authors
  1. Sandra Pavey
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Terry Russell
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Brian Gabrielli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Brian Gabrielli.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pavey, S., Russell, T. & Gabrielli, B. G2 phase cell cycle arrest in human skin following UV irradiation. Oncogene 20, 6103–6110 (2001). https://doi.org/10.1038/sj.onc.1204707

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1204707

Keywords

This article is cited by

Search

Advanced search

Quick links