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Multi-faceted analyses of a highly metastatic human lung cancer cell line NCI-H460-LNM35 suggest mimicry of inflammatory cells in metastasis

Oncogene volume 20, pages 4228–4234 (2001)Cite this article

Abstract

This study established and characterized low-metastatic revertant and parental clones of a highly metastatic human lung cancer cell line, NCI-H460-LNM35 (hereafter referred to as LNM35). Expression-profiling analysis revealed that up-regulation of various proinflammatory cytokines and angiogenic chemotactic chemokines was present in LNM35. Further, while COX-2 itself is known to be inducible in inflammation, COX-2 expression levels correlated well with the capabilities of these clones for not only in vitro motility and invasion but also in vivo metastasis, and COX-2 inhibitors were shown for the first time to reduce lung cancer metastasis in vivo. These findings suggest that lung cancer cells may mimic inflammatory cells in the process of metastasis.

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Acknowledgements

We would like to thank K Matsuo for his advice on the biostatistical analyses. We are also grateful to H Konishi and Y Yatabe for their critical reading of this manuscript. This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science and Culture of Japan.

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Authors and Affiliations

  1. Division of Molecular Oncology, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, Aichi, 464-8681, Japan

    Ken-ichi Kozaki, Katsumi Koshikawa, Yoshio Tatematsu, Hiroko Saito, Hirotaka Osada & Takashi Takahashi

  2. Department of Internal Medicine, Aichi Cancer Center Hospital, Nagoya, Aichi, 464-8681, Japan

    Toyoaki Hida

  3. Department of Basic Gerontology, National Institute for Longevity Sciences, Ohbu, Aichi, 474-8522, Japan

    Osamu Miyaishi

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  1. Ken-ichi Kozaki
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Correspondence to Takashi Takahashi.

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Kozaki, Ki., Koshikawa, K., Tatematsu, Y. et al. Multi-faceted analyses of a highly metastatic human lung cancer cell line NCI-H460-LNM35 suggest mimicry of inflammatory cells in metastasis. Oncogene 20, 4228–4234 (2001). https://doi.org/10.1038/sj.onc.1204561

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