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p53 contains a DNA break-binding motif similar to the functional part of BRCT-related region of Rb

Oncogene volume 20, pages 2859–2867 (2001)Cite this article

Abstract

The BRCT regions are two repeating structures at BRCA1 carboxyl-terminus and are ubiquitous in some proteins involved in DNA repair and cell cycle checkpoints. It was shown that BRCTs of TopBP1, BRCA1, and BRCT-Ws of Rb bound DNA ends and breaks. We indicate here that the C-terminus of p53 tumor suppressor contains a DNA binding motif (residues 327–333 in human), whose features are similar to those of the part of BRCT-W in Rb with DNA binding activity. The short motif was required for the gel retardation activity of DNA fragments, since residues 311–333 showed the activity while residues 311–326 showed no activity. Significant numbers of total p53 and its fragments with the motif formed multimerizing complexes and associated with DNA ends and breaks. These results suggest the common presence of DNA binding motifs that can recognize DNA ends or damages in major tumor suppressors, Rb, BRCA1 and p53. The oncogenic activity of p53 C-terminus (residues 311–393) required both the DNA damage recognition motif and the repair enzyme-associating domain.

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Acknowledgements

This study was supported by the Program for Promotion of Fundamental Studies in Health Sciences of the Organization for Pharmaceutical Safety and Research of Japan.

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Authors and Affiliations

  1. Laboratory of Biomedical Research, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, 113-0032, Tokyo, Japan

    Kazuhiko Yamane & Takashi Tsuruo

  2. Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, 1-37-1, Kami-Ikebukuro, Toshima-ku, 170-8455, Tokyo, Japan

    Kazuhiko Yamane & Takashi Tsuruo

  3. Division of Biomolecular Imaging, 4PRESTO, Japan Science and Technology Corporation, Kawaguchi, 332-0012, Saitama-ken, Japan

    Eisaku Katayama

  4. Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan

    Eisaku Katayama

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Yamane, K., Katayama, E. & Tsuruo, T. p53 contains a DNA break-binding motif similar to the functional part of BRCT-related region of Rb. Oncogene 20, 2859–2867 (2001). https://doi.org/10.1038/sj.onc.1204408

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  • DOI: https://doi.org/10.1038/sj.onc.1204408

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