Abstract
SNT/FRS2 is a lipid anchored docking protein that contains an amino-terminal myristylation signal, followed by a phosphotyrosine-binding (PTB) domain and a carboxy-terminal region with multiple tyrosine residues. Here we show that the SNT/FRS2 PTB domain binds to RET receptor tyrosine kinase activated by glial cell line-derived neurotrophic factor (GDNF) or multiple endocrine neoplasia (MEN) 2 mutations. Analyses by site directed-mutagenesis revealed that it binds to tyrosine 1062 in RET that is also known to be a binding site for the SHC adaptor protein. Whereas SHC bound to RET was associated with GRB2 and GAB1 proteins, SNT/FRS2 was associated with GRB2 only, suggesting that SNT/FRS2 is involved mainly in the activation of the RAS/mitogen activated protein kinase (MAPK) pathway but not the phosphatidylinositol 3-kinase (PI3-K)/AKT pathway. In addition, phosphorylated SNT/FRS2 appeared to directly complex with SHP-2 tyrosine phosphatase. These results suggest that tyrosine 1062 in RET provides a site for the interaction of multiple signaling molecules and that the balance of SHC and SNT/FRS2 binding may affect the nature of the intracellular signaling for cell proliferation, differentiation and survival induced by activated RET.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Airaksinen MS, Titievsky A, Saarma M . 1999 Mol. Cell. Neurosci. 13: 313–325
Arighi E, Alberti L, Torriti F, Ghizzoni S, Rizzetti MG, Pelicci G, Pasini B, Bongarzone I, Piutti C, Pierotti MA, Borrello MG . 1997 Oncogene 14: 773–782
Asai N, Murakami H, Iwashita T, Takahashi M . 1996 J. Biol. Chem. 271: 17644–17649
Besset V, Scott RP, Ibáñez CF . 2000 J. Biol. Chem. 275: 39159–39166
Califano D, Rizzo C, D'Alessio A, Colucci-D'Amato GL, Calì G, Bartoli PC, Santelli G, Vecchio G, De Franciscis V . 2000 J. Biol. Chem. 275: 19297–19305
Craparo A, O'Neill TJ, Gustafson TA . 1995 J. Biol. Chem. 270: 15639–15643
DeVita G, Melillo RM, Carlomagno F, Visconti R, Castellone MD, Bellacosa A, Billaud M, Fusco A, Tsichilis PN, Santoro M . 2000 Cancer Res. 60: 3727–3731
Durick K, Gill GN, Taylor SS . 1998 Mol. Cell. Biol. 18: 2298–2308
Geneste O, Bidaud C, De Vita G, Hofstra RMW, Tartare-Deckert S, Buys CHCM, Lenoir GM, Santro M, Billaud M . 1999 Hum. Mol. Genet. 8: 1989–1999
Gustafson TA, He W, Craparo A, Schaub CD, O'Neill TJ . 1995 Mol. Cell. Biol. 15: 2500–2508
Hadari YR, Kouhara H, Lax I, Schlessinger J . 1998 Mol. Cell. Biol. 20: 3966–3973
Hayashi H, Ichihara M, Iwashita T, Murakami H, Shimono Y, Kawai K, Kurokawa K, Murakumo Y, Imai T, Funahashi H, Nakao A, Takahashi M . 2000 Oncogene 19: 4469–4475
He W, Craparo A, Zhu Y, O'Neill TJ, Wang L-M, Pierce JH, Gustafson TA . 1996 J. Biol. Chem. 271: 11641–11645
Ishiguro Y, Iwashita T, Murakami H, Asai N, Iida K, Goto H, Hayakawa T, Takahashi M . 1999 Endocrinology 140: 3992–3998
Iwashita T, Asai N, Murakami H, Matsuyama M, Takahashi M . 1996 Oncogene 12: 481–487
Iwashita T, Kato M, Murakami H, Asai N, Ishiguro Y, Ito S, Iwata Y, Kawai K, Asai M, Kurokawa K, Kajita H, Takahashi M . 1999 Oncogene 18: 3919–3922
Iwashita T, Murakami H, Kurokawa K, Kawai K, Miyauchi A, Futami H, Qiao S, Ichihara M, Takahashi M . 2000 Biochem. Biophys. Res. Commun. 268: 804–808
Kouhara H, Hadari YR, Spivak-Kroizman T, Schilling J, Bar-Sagi D, Lax I, Schlessinger J . 1997 Cell 89: 693–702
Lorenzo MJ, Gish GD, Houghton C, Stonehouse TJ, Pawson T, Ponder BAJ, Smith DP . 1997 Oncogene 14: 763–771
Meakin SO, MacDonald JIS, Gryz EA, Kubu CJ, Verdi JM . 1999 J. Biol. Chem. 274: 9861–9870
Murakami H, Iwashita T, Asai N, Iwata Y, Narumiya S, Takahashi M . 1999a Oncogene 18: 1975–1982
Murakami H, Iwashita T, Asai N, Shimono Y, Iwata Y, Kawai K, Takahashi M . 1999b Biochem. Biophys. Res. Commun. 262: 68–75
Ohiwa M, Murakami H, Iwashita T, Asai N, Iwata Y, Imai T, Funahashi H, Takagi H, Takahashi M . 1997 Biochem. Biophys. Res. Commun. 237: 747–751
Ong SH, Guy GR, Hadari YR, Laks S, Gotoh N, Schlessinger J, Lax I . 2000 Mol. Cell. Biol. 20: 979–989
Segouffin-Cariou C, Billaud M . 2000 J. Biol. Chem. 275: 3568–3576
Rabin SJ, Vaughn C, Kaplan DR . 1993 Mol. Cell. Biol. 13: 2203–2213
Takahashi M, Buma Y, Iwamoto T, Inaguma Y, Ikeda H, Hiai H . 1988 Oncogene 3: 571–578
Takahashi M, Buma Y, Taniguchi M . 1991 Oncogene 6: 297–301
Wright JH, Drueckes P, Bartoe J, Zhao Z, Shen S-H, Krebs EG . 1997 Mol. Biol. Cell. 8: 1575–1585
Xu H, Lee KW, Goldfarb M . 1998 J. Biol. Chem. 273: 17987–17990
Acknowledgements
We are grateful to K Imaizumi and M Kozuka for their technical assistance. This work was supported in part by a grant-in-aid for COE (Center of Excellence) research from the Ministry of Education, Science, Sports and Culture of Japan.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kurokawa, K., Iwashita, T., Murakami, H. et al. Identification of SNT/FRS2 docking site on RET receptor tyrosine kinase and its role for signal transduction. Oncogene 20, 1929–1938 (2001). https://doi.org/10.1038/sj.onc.1204290
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1204290
Keywords
This article is cited by
-
Optogenetic dissection of RET signaling reveals robust activation of ERK and enhanced filopodia-like protrusions of regenerating axons
Molecular Brain (2023)
-
Intracellular RET signaling pathways activated by GDNF
Cell and Tissue Research (2020)
-
Frs2 alpha
AfCS-Nature Molecule Pages (2009)
-
Identification of multiple SNT-binding sites on NPM-ALK oncoprotein and their involvement in cell transformation
Oncogene (2007)
-
Expression of the SNT-1/FRS2 phosphotyrosine binding domain inhibits activation of MAP kinase and PI3-kinase pathways and antiestrogen resistant growth induced by FGF-1 in human breast carcinoma cells
Oncogene (2006)