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Genetic analysis of ETS genes in C. elegans

Oncogene volume 19, pages 6400–6408 (2000)Cite this article

Abstract

The recent completion of the Caenorhabditis elegans genome has revealed that this nematode worm has 10 members of the ETS gene family. Isolation and analysis of C. elegans mutants and subsequent screens to identify interacting genes can proceed very quickly in this model organism. Molecular genetic analysis of the receptor tyrosine kinase-Ras-MAP kinase signaling pathway in C. elegans identified the ETS family transcription factor Lin-1 as a nuclear effector of this evolutionarily conserved signal transduction pathway. Here we review classical genetic approaches used to discover the role of Lin-1 in the Ras-MAP kinase signaling pathway and describe new technologies that can be applied to the analyses of signaling pathways and transcription factor regulatory networks in C. elegans.

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Acknowledgements

We wish to thank Joseph Culotti and members of his laboratory at the Samuel Lunenfeld Research Institute, specifically Peter Roy, Lijia Zhang, and Bruce Nash for advice, technical assistance and access to their mutagenized C. elegans library. This work was supported by grants from the National Cancer Institute of Canada (NCIC) and Terry Fox Cancer Foundation to A Bernstein.

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Authors and Affiliations

  1. Program of Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, M5G 1X5, Ontario, Canada

    Adam H Hart, Roddie Reventar & Alan Bernstein

  2. Department of Molecular and Medical Genetics, University of Toronto, M5S 1A8, Canada

    Alan Bernstein

  3. Canadian Institutes of Health Research, 410 Laurier Avenue West, Postal Locator 4209A, Ottawa, K1A 0W9, Ontario, Canada

    Alan Bernstein

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Hart, A., Reventar, R. & Bernstein, A. Genetic analysis of ETS genes in C. elegans. Oncogene 19, 6400–6408 (2000). https://doi.org/10.1038/sj.onc.1204040

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