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Identification and characterization of genes upregulated in cells transformed by v-Jun

Oncogene volume 19, pages 3537–3545 (2000)Cite this article

Abstract

The transcription factor Jun (c-Jun) functions as a recipient of extracellular growth signals and converts them into patterns of gene expression. An oncogenic variant of c-Jun was isolated from the acutely transforming retrovirus ASV17. Overexpression of this viral Jun (v-Jun) induces transformation of chicken embryo fibroblasts (CEF) in culture and fibrosarcomas in chickens. v-Jun is a constitutively active form of c-Jun and transforms cells presumably by deregulating the expression of specific target genes. In this report, we describe six genes whose transcripts are upregulated in v-Jun-transformed CEF. Three of these genes show homology to known mammalian genes, to MAP kinase phosphatase 2 (MKP-2), to reversion-induced LIM protein (RIL) and to cytokine-inducible SH2-containing protein (CIS). Northern blot analysis, using CEF infected with various Jun mutants or an estrogen-regulatable Jun chimera, revealed distinct induction patterns of individual targets by v-Jun. The chicken RIL homolog showed an expression pattern tightly correlated with the activity of v-Jun. Its expression is also transformation-dependent, suggesting a role for this gene in v-Jun transformation. The newly identified v-Jun targets can serve as molecular markers in the v-Jun transformation process.

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Acknowledgements

This work was supported by the Department of Health and Human Services Research Grant CA 42564 from the National Cancer Institute. We thank Douglas Geerdes for preparing primary CEF, Stephen Hughes, George Vande Woude, Ivan Bottoli, Martin Goller and Ulrich Kruse for providing reagents, Susan Burke for preparation of the manuscript and members of the Vogt laboratory for helpful discussions. This is manuscript number 12709-MEM at The Scripps Research Institute.

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  1. Shu-ling Fu

    Present address: Institute of Molecular and Cellular Biology, Tzu-Chi College of Medicine and Humanities, Hualien, Taiwan

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  1. Department of Molecular and Experimental Medicine, The Scripps Research Institute, BCC239, 10550 North Torrey Pines Road, La Jolla, 92037, California, CA, USA

    Shu-ling Fu, Anke Waha & Peter K Vogt

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Fu, Sl., Waha, A. & Vogt, P. Identification and characterization of genes upregulated in cells transformed by v-Jun. Oncogene 19, 3537–3545 (2000). https://doi.org/10.1038/sj.onc.1203691

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