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  • Original Paper
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Retinoblastoma susceptibility protein, Rb, possesses multiple BRCT-Ws, BRCA1 carboxyl-terminus-related W regions with DNA break-binding activity

Oncogene volume 19, pages 1982–1991 (2000)Cite this article

Abstract

The BRCT region, the carboxyl-terminus of BRCA1 (the breast cancer susceptibility gene 1 product), is ubiquitous in several proteins that participate in cell cycle checkpoints and DNA repair. We have previously shown that the BRCT regions of TopBP1 (DNA topoisomerase II binding protein 1) and BRCA1 bound DNA breaks. A BRCT-related region, BRCT-W1, in the retinoblastoma susceptibility gene product (Rb) also could bind DNA fragments, independently of DNA sequences. Five BRCT-W regions were found in the Rb family. All BRCT-Ws of Rb bound DNA fragments. Electron microscopy and treatment with an exonuclease showed that BRCT-Ws bound double-strand DNA breaks. Since some BRCTs are exceptional common relating elements in tumor suppression, our findings reveal novel aspects of the tumor suppression mechanism.

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Acknowledgements

This study was supported by the Program for Promotion of Fundamental Studies in Health Sciences of the Organization for Pharmaceutical Safety and Research of Japan. K Yamane was a postdoctoral fellow supported by the program. This work is also supported by a special grant for Advanced Research on Cancer from Ministry of Education Science, Sports and Culture, Japan.

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Authors and Affiliations

  1. Laboratory of Biomedical Research, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, 113-0032, Tokyo, Japan

    Kazuhiko Yamane & Takashi Tsuruo

  2. Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, 1-37-1, Kami-Ikebukuro, 170-8455, Toshima-ku, Tokyo, Japan

    Kazuhiko Yamane & Takashi Tsuruo

  3. Department of Fine Morphology, Institute of Medical Science The University of Tokyo, 4-6-1, Shirokanedai, 108-8639, Minato-ku, Tokyo, Japan

    Eisaku Katayama

  4. The Institute of Physical and Chemical Research (RIKEN) 2-1, Hirosawa, 351-0198, Wako-shi, Saitama, Japan

    Kaoru Sugasawa

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Yamane, K., Katayama, E., Sugasawa, K. et al. Retinoblastoma susceptibility protein, Rb, possesses multiple BRCT-Ws, BRCA1 carboxyl-terminus-related W regions with DNA break-binding activity. Oncogene 19, 1982–1991 (2000). https://doi.org/10.1038/sj.onc.1203528

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