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  • Original Article
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Human frizzled 1 interacts with transforming Wnts to transduce a TCF dependent transcriptional response

Oncogene volume 18, pages 5959–5966 (1999)Cite this article

Abstract

The human homologue of fz1 (Hfz1) was cloned from a cDNA library. Hfz1 was shown to couple to Wnt signal transduction pathways by its ability to enhance Wnt induced TCF dependent transcription in both autocrine and paracrine modes. Enhanced TCF dependent signaling was dose dependent with respect to both Wnt-3A and Hfz1. Moreover, Hfz1 deletion mutants with truncated carboxy termini showed markedly reduced capacity to enhance Wnt signal transduction. Specificity was demonstrated with respect to signal transduction by different Wnts. While Wnt-3a, -3, -1 and to a lesser extent Wnt-2 cooperated with Hfz1 in the paracrine assay for TCF dependent signaling, neither Wnt-4, -5a, -5b, -6, -7a nor -7b did so, despite similar levels of expression. However, coimmunoprecipitation of Hfz1 with both Wnt-3a and Wnt-5a indicated that TCF dependent signaling in response to Wnts is not determined solely by their ability to bind the receptor. All of these findings provide strong evidence that Hfz1 is a functional partner for certain Wnts in inducing TCF dependent transcription.

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Acknowledgements

This research was supported by NIH R01-CA71672 and a fellowship to Makoto Igarashi from the Leo and Julia Forchheimer Foundation.

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Authors and Affiliations

  1. Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, 69978, Israel

    Arnona Gazit & Abraham Yaniv

  2. Derald H. Ruttenberg Cancer Center, The Mount Sinai School of Medicine, New York, 10029, NY, USA

    Anna Bafico, Tata Pramila, Makoto Igarashi & Stuart A Aaronson

  3. Department of Pathology and Center for Reproductive Sciences, Columbia University, College of Physicians and Surgeons, New York, 10032, NY, USA

    Jan Kitajewski

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  1. Arnona Gazit
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Gazit, A., Yaniv, A., Bafico, A. et al. Human frizzled 1 interacts with transforming Wnts to transduce a TCF dependent transcriptional response. Oncogene 18, 5959–5966 (1999). https://doi.org/10.1038/sj.onc.1202985

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