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cDNA expression array reveals heterogeneous gene expression profiles in three glioblastoma cell lines

Oncogene volume 18, pages 2711–2717 (1999)Cite this article

Abstract

Tumor cell lines are an indispensable tool for cancer research. However, among cell lines of the same pathological group, heterogeneity has been detected in gene expression, gene mutation, and cellular response to various treatments. In this study, we systematically investigated the extent of heterogeneity of gene expression in three glioblastoma cell lines using cDNA array technology in which the expression of 588 cellular genes is studied simultaneously. Comparison of the expression profiles revealed substantial qualitative and quantitative heterogeneity. Among the 588 genes, 197 genes were expressed in all three lines and 56 genes were not expressed in any of the three lines; total of 222 genes were expressed in only two of the three cell lines, and 113 genes were expressed in only one of the three cell lines. These results provide molecular evidence that cell lines of the same pathological origin can be highly heterogeneous.

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References

  • Dimery IW, Ross DD, Testa JR, Gupta SK, Felsted RL, Pollak A and Bachur NR. . 1983 Exp. Hematol. 11: 601–610.

  • El-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW and Vogelstein B. . 1993 Cell 75: 817–825.

    Article  CAS  Google Scholar 

  • Fearon ER and Vogelstein B. . 1990 Cell 61: 759–767.

    Article  CAS  Google Scholar 

  • Grau AM, Zhang L, Wang W, Ruan S, Evans DB, Abbruzzese JL, Zhang W and Chiao PJ . 1997 Cancer Res. 57: 3929–3934.

  • Hahn SA Schutte M, Hoque AT, Moskaluk CA, da Costa LT, Rozenblum E, Weinstein CL, Fischer A, Yeo CJ, Hruban RH and Kern SE. . 1996 Science 271: 350–353.

    Article  CAS  Google Scholar 

  • Holliday RH. . 1989 Trends Genet. 5: 42–45.

  • Hollstein MC, Sidransky D, Vogelstein B and Harris CC. . 1991 Science 252: 49–53.

    Article  CAS  Google Scholar 

  • Jung J-M, Li H, Kobayashi T, Kyritsis AP, Langford LA, Bruner JM, Levin VA and Zhang W. . 1995 Cell Growth Differ. 6: 909–913.

  • Kamb A, Shattuck-Eidens D, Eeles R, Liu Q, Gruis NA, Ding W, Hussey C, Tran T, Miki Y, Weaver-Feldhaus J, McClur M, Aitken JF, Anerson DE, Bergman W, Frants R, Goldgar DE, Green A, MacLennan R, Martin NG, Meyer LJ, Youl P, Zone JJ, Skolnick MH and Cannon-Albright LA. . 1994 Nat. Genet. 8: 22–26.

  • Kastan MB, Onyekwere O, Sidransky D, Vogelstein B and Craig RW. . 1991 Cancer Res. 51: 6304–6311.

  • Nowell PC. . 1976 Science 194: 23–28.

    Article  CAS  Google Scholar 

  • Nowell PC. . 1986 Cancer Res. 46: 2203–2207.

  • Owen-Schaub LB, Zhang W, Cusack J, Angelo LS, Santee SM, Fujiwara T, Roth JA, Deisseroth AB, Zhang W-W, Kruzel E and Radinsky R. . 1995 Mol. Cell. Biol. 15: 3032–3040.

    Article  CAS  Google Scholar 

  • Van Meir EG, Kikuchi T, Tada M, Li H, Diserens AC, Wojcik BE, Huang HJ, Friedmann T, de Tribolet N and Cavenee WK. . 1994 Cancer Res. 54: 649–652.

  • Weinberg RA. . 1989 Cancer Res. 49: 3713–3721.

  • Zhang W, Grasso L, McClain CD, Gambel AM, Cha Y, Travali S, Deisseroth AB and Mercer WE. . 1995 Cancer Res. 55: 668–674.

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Acknowledgements

This work was partially supported by NIH Grants CA67987 and CA55164. Chang Hun Rhee is a visiting fellow partially supported by the Korean Cancer Center Hospital. We thank Stephanie Deming for editorial assistance with the manuscript.

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Authors and Affiliations

  1. Department of Neuro-Oncology, The University of Texas MD Anderson Cancer Center, Houston, 77030, Texas, USA

    Chang Hun Rhee, James Jabbur, Maribelis Ruiz, Yu Yang & Wei Zhang

  2. Department of Biomathematics, The University of Texas MD Anderson Cancer Center, Houston, 77030, Texas, USA

    Kenneth Hess

  3. Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, 77030, Texas, USA

    Gregory N Fuller

  4. Clontech Laboratories, Inc., Palo Alto, 94303-4230, California, USA

    Stephen Chen & Alex Chenchik

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  1. Chang Hun Rhee
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  2. Kenneth Hess
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Rhee, C., Hess, K., Jabbur, J. et al. cDNA expression array reveals heterogeneous gene expression profiles in three glioblastoma cell lines. Oncogene 18, 2711–2717 (1999). https://doi.org/10.1038/sj.onc.1202623

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  • DOI: https://doi.org/10.1038/sj.onc.1202623

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