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  • Original Paper
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Search for oncogenic regulators in an autocrine tumor model using differential display PCR: Identification of novel candidate genes including the calcium channel mtrp6

Oncogene volume 18, pages 1487–1494 (1999)Cite this article

Abstract

A hemopoietic multistep tumor model, in which IL-3 dependent PB-3c mast cells, following expression of v-H-ras progress in vivo to IL-3 producing autocrine tumors has previously been established. Central for this oncogenic progression is a recessive step, which is reversible by cell fusion and leads to stabilization of IL-3 mRNA with concomitant activation of the autocrine loop. Comparing the IL-3 dependent PB-3c and the IL-3 autocrine V2D1 tumor cells with differential display PCR revealed 12 differentially expressed genes of which eight were upregulated and four downregulated in the tumor. They included four proteases (mouse mast cell protease 2, granzyme B, pepsinogen F and serine protease 1) and two metabolic enzymes (adenine phosphoribosyltransferase and fructose1,6-bisphosphatase). For validation, expression of the identified genes was tested in independent PB-3c precursor clones and their tumor derivatives. Expression of an endogenous retroviral IAP element and three unknown transcripts were consistently upregulated in all tumor lines. In somatic cell hybrids, two of these unknown cDNAs showed a dominant and one a recessive expression pattern. One transcript, expressed in the precursor but downregulated in the tumor cells, was cloned and identified as the murine calcium channel mtrp6.

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References

  • Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W and Lipman DJ. . 1997 Nucl. Acids. Res. 25: 3389–3402.

  • Ball PE, Conroy MC, Heusser CH, Davis JM and Conscience JF. . 1983 Differentiation 24: 74–78.

  • Banholzer R, Nair AP, Hirsch HH, Ming XF and Moroni C. . 1997 Mol. Cell. Biol. 17: 3254–3260.

  • Bauer D, Muller H, Reich J, Riedel H, Ahrenkiel V, Warthoe P and Strauss M. . 1993 Nucl. Acids Res. 21: 4272–4280.

  • Bertioli DJ, Schlichter UH, Adams MJ, Burrows PR, Steinbiss HH and Antoniw JF. . 1995 Nucl. Acids Res. 23: 4520–4523.

  • Boulay G, Zhu X, Peyton M, Jiang M, Hurst R, Stefani E and Birnbaumer L. . 1997 J. Biol. Chem. 272: 29672–29680.

  • Brunet JF, Denizot F, Suzan M, Haas W, Mencia-Huerta JM, Berke G, Luciani MF and Golstein P. . 1987 J. Immunol. 138,: 4102–4105.

  • Brunet JF, Dosseto M, Denizot F, Mattei MG, Clark WR, Haqqi TM, Ferrier P, Nabholz M, Schmitt-Verhulst AM, Luciani MF and Golstein P. . 1986 Nature 322: 268–271.

  • Buess M, Moroni C and Hirsch HH. . 1997 Nucl. Acids Res. 25: 2233–2235.

  • Burnay MM, Python CP, Vallotton MB, Capponi AM and Rossier MF. . 1994 Endocrinology 135: 751–758.

  • Chenchik A, Diachenko L, Moqadam F, Tarabykin V, Lukyanov S and Siebert PD. . 1996 Biotechniques 21: 526–534.

  • Darmon AJ, Ley TJ, Nicholson DW and Bleackley RC. . 1996 J. Biol. Chem. 271: 21709–21712.

  • Diamantis ID, Nair AP, Hirsch HH and Moroni C. . 1989 Proc. Natl, Acad. Sci. USA 86: 9299–9303.

  • Fanger CM, Hoth M, Crabtree GR and Lewis RS. . 1995 J. Cell. Biol. 131: 655–667.

  • Gough NM. . 1988 Anal. Biochem. 173: 93-95.

  • Hardie RC and Minke B. . 1992 Neuron 8: 643–651.

  • Hawley RG, Shulman MJ and Hozumi N. . 1984 Mol. Cell. Biol. 4: 2565–2572.

  • Hirsch HH, Nair AP, Backenstoss V and Moroni C. . 1995 J. Biol. Chem. 270: 20629–20635.

  • Hirsch HH, Nair AP and Moroni C. . 1993 J. Exp. Med. 178: 403–411.

  • Karasuyama H and Melchers F. . 1988 Eur. J. Immunol. 18: 97–104.

  • Kuff EL and Lueders KK. . 1988 Adv. Cancer Res. 51: 183–276.

  • Liang P, Averboukh L, Keyomarsi K, Sager R and Pardee AB. . 1992 Cancer Res. 52: 6966–6968.

  • Liang P, Averboukh L and Pardee AB. . 1993 Nucl. Acids Res. 21: 3269–3275.

  • Liang P and Pardee AB. . 1992 Science 257: 967–971.

  • Liang P and Pardee AB. . 1995 Curr. Opin. Immunol. 7: 274–280.

  • Liotta LA, Steeg PS and Stetler-Stevenson WG. . 1991 Cell 64: 327–336.

  • McNeil HP, Austen KF, Somerville LL, Gurish MF and Stevens RL. . 1991 J. Biol. Chem. 266: 20316–20322.

  • Mirza H, Schmidt VA, Derian CK, Jesty J and Bahou NF. . 1997 Blood 90: 3914–3922.

  • Nair AP, Diamantis ID, Conscience JF, Kindler V, Hofer P and Moroni C. . 1989 Mol. Cell. Biol. 9: 1183–1190.

  • Nair AP, Hahn S, Banholzer R, Hirsch HH and Moroni C. . 1994 Nature 369: 239–242.

  • Nair AP, Hirsch HH and Moroni C. . 1992 Oncogene 7: 1963–1972.

  • Nomura M, Takihara Y, Yasunaga T and Shimada K. . 1994 FEBS Lett. 348: 201–205.

  • Philipp S, Cavalie A, Freichel M, Wissenbach U, Zimmer S, Trost C, Marquart A, Murakami M and Flockerzi V. . 1996 EMBO J. 15: 6166–6171.

  • Sager R, Anisowicz A, Neveu M, Liang P and Sotiropoulou G. . 1993 Faseb J. 7: 964–70.

  • Serafin WE, Reynolds DS, Rogelj S, Lane WS, Conder GA, Johnson SS, Austen KF and Stevens RL. . 1990 J. Biol. Chem. 265: 423–429.

  • Shaw G and Kamen R. . 1986 Cell 46: 659–667.

  • Stevens RL and Austen KF. . 1989 Immunol. Today 10: 381–386.

  • Thomas PS. . 1980 Proc. Natl. Acad. Sci. USA 77: 5201–5205.

  • Turker MS, Cooper GE and Bishop PL. . 1993 J. Mol. Evol. 36: 31–40.

  • Wodnar-Filipowicz A, Heusser CH and Moroni C. . 1989 Nature 339: 150–152.

  • Wodnar-Filipowicz A and Moroni C. . 1990 Proc. Natl. Acad. Sci. USA 87: 777–781.

  • Zhu X, Jiang M, Peyton M, Boulay G, Hurst R, Stefani E and Birnbaumer L. . 1996 Cell 85: 661–671.

  • Zweifach A and Lewis RS. . 1993 Proc. Natl. Acad. Sci. USA 90: 6295–6299.

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Acknowledgements

We thank our colleagues Drs L Brennan, X-F Ming, A Nair, G Stoecklin and A Wyss for helpful comments and discussion. M Buess is a fellow of the Roche Research Foundation. This work was in part supported by the Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung through grant 31-40816.94.

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  1. Institute for Medical Microbiology, University of Basel, Petersplatz 10, Basel, CH-4003, Switzerland

    Martin Buess, Olivier Engler, Hans H Hirsch & Christoph Moroni

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Buess, M., Engler, O., Hirsch, H. et al. Search for oncogenic regulators in an autocrine tumor model using differential display PCR: Identification of novel candidate genes including the calcium channel mtrp6. Oncogene 18, 1487–1494 (1999). https://doi.org/10.1038/sj.onc.1202445

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