Abstract
Neogenin is a member of the N-CAM family of cell adhesion molecules and is closely related to the DCC tumor suppressor gene product. Recently, it has been demonstrated that the DCC/Neogenin subfamily plays a key role in axonal guidance within the embryonic nervous system, however little is known about the function of DCC or Neogenin in non-neuronal tissues in vertebrates. To gain an understanding of Neogenin function outside of the nervous system we have cloned and sequenced the mouse homologue of Neogenin. We describe three alternatively spliced exons within the extracellular domain of Neogenin and a fourth alternatively spliced exon within the cytoplasmic domain. We further demonstrate that three of these alternatively spliced exons are developmentally regulated. Analysis of Neogenin mRNA expression showed that two distinct Neogenin transcripts are expressed at significant levels in a broad spectrum of adult mouse tissues and throughout the mid to late stages of embryogenesis. In situ hybridization studies on day 15.5 mouse embryos revealed that Neogenin is expressed widely throughout the developing mouse embryo, in both neuronal and non-neuronal tissues. These observations suggests that Neogenin may play an integral role in regulating differentiation programmes and/or cell migration events within many embryonic and adult tissues.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Keeling, S., Gad, J. & Cooper, H. Mouse Neogenin, a DCC-like molecule, has four splice variants and is expressed widely in the adult mouse and during embryogenesis. Oncogene 15, 691–700 (1997). https://doi.org/10.1038/sj.onc.1201225
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1201225
Keywords
This article is cited by
-
Re-evaluation of the myoepithelial cells roles in the breast cancer progression
Cancer Cell International (2022)
-
Neogenin recruitment of the WAVE regulatory complex maintains adherens junction stability and tension
Nature Communications (2016)
-
Netrin-1 Contributes to Myelinated Afferent Fiber Sprouting and Neuropathic Pain
Molecular Neurobiology (2016)
-
Protogenin, a new member of the immunoglobulin superfamily, is implicated in the development of the mouse lower first molar
BMC Developmental Biology (2010)
-
Molecular Evolution of Hemojuvelin and the Repulsive Guidance Molecule Family
Journal of Molecular Evolution (2007)