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Effect of oral oleoyl-estrone treatment on plasma lipoproteins and tissue lipase activities of Zucker lean and obese female rats

International Journal of Obesity volume 26, pages 618–626 (2002)Cite this article

Abstract

Objective: To study the effect of oral oleoyl-estrone on the plasma lipoprotein profile and tissue lipase activities in order to determine the handling of circulating lipids by adipose tissue, liver and muscle of obese female rats.

Design: Lean (Fa/?) and obese (fa/fa) female Zucker rats treated for 10 days with a daily gavage of 0.2 ml sunflower oil containing 0 (controls) or 10 µmol/kg of oleoyl-estrone. After sacrifice, samples of tissues and plasma were taken.

Measurements: Plasma lipoprotein classes and composition; lipoprotein lipase and hepatic lipase activities in plasma, liver, skeletal muscle and periovaric and mesenteric white adipose tissue (WAT).

Results: Oleoyl-estrone decreased plasma cholesterol (mainly in HDLs: 76%) of lean rats, but dramatically decreased all lipid classes in obese rats, in which chylomicra and VLDL lost most of their triacylglycerols (95 and 81%, respectively). Hepatic lipase activity decreased markedly with oleoyl-estrone in all groups, both in plasma (79% lean, 100% obese) and liver (62% in both groups). Lipoprotein lipase activity was largely unchanged by oleoyl-estrone in lean rats, but in the obese it decreased in WAT (82% in periovaric, and 49% in mesenteric), and increased in plasma (×4) and in skeletal muscle (×5); liver levels showed no change.

Conclusions: The shift observed in obese rats from a decrease in liver and WAT lipoprotein lipase and hepatic lipase activities to an increase in muscle lipoprotein lipase is coincident with the hypolipemic effect of oleoyl-estrone, especially in obese rats, and indicates that muscle is a key site for the disposal of endogenous fat mobilized due to oleoyl-estrone treatment.

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Acknowledgements

This work was carried out with grant 2FD97-0233 from the Government of Spain. Thanks are given to Dr Angelo Pietrobelli for his critical review of the manuscript and essential contribution to the organization of the materials. The linguistic correction of the text by Robin Rycroft from the SAL, University of Barcelona, is gratefully acknowledged.

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Authors and Affiliations

  1. Centre Especial de Recerca en Nutrició i Ciència dels Aliments, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain

    M Blay, J Peinado-Onsurbe, MM Grasa, M Díaz-Silva, JA Fernandez-López, X Remesar & M Alemany

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  1. M Blay
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Correspondence to M Alemany.

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Blay, M., Peinado-Onsurbe, J., Grasa, M. et al. Effect of oral oleoyl-estrone treatment on plasma lipoproteins and tissue lipase activities of Zucker lean and obese female rats. Int J Obes 26, 618–626 (2002). https://doi.org/10.1038/sj.ijo.0801985

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