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Characterization of mouse GBP28 and its induction by exposure to cold

International Journal of Obesity volume 25, pages 75–83 (2001)Cite this article

Abstract

OBJECTIVE: To investigate whether the expression of the novel adipose tissue-specific protein GBP28 in adipose tissue and serum are altered in mice under a variety of conditions.

DESIGN: Mice were fed a high-fat diet for 4 weeks, fasted for 48 h or exposed at 4°C.

SUBJECTS: C57BL/6J mouse, male, 4–6 weeks old.

MEASUREMENTS: GBP28 mRNA, GBP28 protein, blood glucose, insulin and fad pad weight of the mice.

RESULTS: We first confirmed that the mouse has GBP28 and its characteristics are the same as human GBP28. Serum concentration and mRNA levels of GBP28 significantly increased in the mice exposed to cold.

CONCLUSION: GBP28 may play a role in homeostasis, regulating body temperature and basal metabolic rate in response to changing environmental conditions.

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Acknowledgements

This study was supported in part by Grant-in Aid for Scientific Research 0845761 from the Ministry of Education, Sciences, Sports and Culture of Japan (to MT).

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Authors and Affiliations

  1. Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan

    M Yoda, N-H Choi-Miura & M Tomita

  2. Pharmaceuticals and Medical Devices Evaluation Center, National Institute of Health Sciences, Tokyo, Japan

    Y Nakano

  3. Department of Medicinal Information, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan

    T Tobe

  4. Department of Anatomy, School of Medicine, Showa University, Tokyo, Japan

    S Shioda

Authors
  1. M Yoda
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  2. Y Nakano
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  3. T Tobe
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  4. S Shioda
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  5. N-H Choi-Miura
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  6. M Tomita
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Correspondence to Y Nakano.

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Yoda, M., Nakano, Y., Tobe, T. et al. Characterization of mouse GBP28 and its induction by exposure to cold. Int J Obes 25, 75–83 (2001). https://doi.org/10.1038/sj.ijo.0801482

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  • DOI: https://doi.org/10.1038/sj.ijo.0801482

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