Abstract
OBJECTIVE: To examine the expression of peroxisome proliferator-activated receptor γ (PPARγ) together with CCAAT/enhancer binding protein α (C/EBPα), lipoprotein lipase (LPL) and glucose transporter (GLUT4) mRNA in adipose tissue of rhesus monkeys in relation to obesity.
DESIGN: Cloning of the PPARγ1 and γ2 cDNAs and analysis of PPARγ, C/EBPα, LPL and GLUT4 mRNA levels in the adipose tissue of lean and obese monkeys.
SUBJECTS: 28 rhesus monkeys (Macaca mulatta) with a wide range of body weights (9.2–22.6 kg) and with or without type 2 diabetes.
MEASUREMENTS: Sequence of PPARγ1 and γ2. Tissue distribution of PPARγ1 and γ2. The mRNA levels of PPARγ, C/EBPα, LPL and GLUT4 in adipose tissue. The ratio of PPARγ2 mRNA to total PPARγ mRNA.
RESULTS: The monkey PPARγ2 protein showed 99% identity with the human protein. PPARγ1 mRNA was shown to be expressed in various tissues and most abundantly in adipose tissue. PPARγ2 existed mainly in adipose tissue. A significant correlation between the ratio of PPARγ2 mRNA to total PPARγ mRNA and obesity was observed, whereas total PPARγ mRNA levels showed no significant relationships to obesity. There was also a significant relationship between the ratio of PPARγ2 mRNA to total PPARγ mRNA and fasting plasma insulin concentration. The mRNA levels of C/EBPα, LPL and GLUT4 were highly correlated to that of total PPARγ mRNA. They were also significantly correlated to the mRNA levels of PPARγ1 and PPARγ2.
CONCLUSIONS: The ratio of PPARγ2 mRNA to total PPARγ mRNA is related to obesity in the rhesus monkey and mRNA expression of PPARγ1, PPARγ2, C/EBPα, LPL and GLUT4 appear to be coordinated in vivo.
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Hotta, K., Gustafson, T., Yoshioka, S. et al. Relationships of PPARγ and PPARγ2 mRNA levels to obesity, diabetes and hyperinsulinaemia in rhesus monkeys. Int J Obes 22, 1000–1010 (1998). https://doi.org/10.1038/sj.ijo.0800718
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DOI: https://doi.org/10.1038/sj.ijo.0800718
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