Abstract
A gene sequence for the fowl plague virus (FPV) haemagglutinin molecule has been inserted into a bacterial plasmid such that its transcription is under the control of a promoter derived from the tryptophan operon. Such plasmids direct the synthesis of a protein that reacts specifically with antisera to FPV haemagglutinin. Evidence is also presented that in some cases DNA inserted at the HindIII site of pBR322 is expressed.
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References
Schild, G. C. in Chemoprophylaxis and Virus Infections of the Respiratory Tract Vol. 1 (ed. Oxford, J.S.) 63–101 (CRC Press, Cleveland, 1977).
Kavet, J. thesis, Harvard Univ. (1972).
Drzeniek, R., Seto, J. T. & Rott, R. Biochim. biophys. Acta 128, 547–558 (1966).
Laver, W. G. & Kilbourne, E. D. Virology 30, 493–501 (1966).
Wiley, D. C., Skehel, J. J. & Waterfield, M. D. Virology 79, 446–448 (1977).
Porter, A. G. Nature 282, 471–477 (1979).
Skehel, J. J. & Schild, G. C. Virology 44, 396–408 (1971).
Laver, W. G. Virology 45, 275–288 (1971).
Tacon, W. A., Carey, N. H. & Emtage, J. S. Molec. gen. Genet. (in the press).
Lee, F., Bertrand, K., Bennett, G. & Yanofsky, C. J. molec. Biol. 121, 193–217 (1978).
Steitz, J. A. & Jakes, K. Proc. natn. Acad. Sci. U.S.A. 72, 4734–4738 (1975).
Glover, D. in New Techniques in Biophysics and Cell Biology Vol. 8 (eds Pain, R. H. & Smith, B. J.) 125–145 (Wiley, New York, 1976).
Miller, J. H. Experiments in Molecular Genetics (Cold Spring Harbor Laboratory, New York, 1976).
Weissman, C. & Boll, W. Nature 261, 428–429 (1976).
Sharp, P. A., Sugden, B. & Sambrook, J. Biochemistry 12, 3055–3063 (1973).
Sutcliffe, J. G. Proc. natn. Acad. Sci. U.S.A. 75, 3737–3741 (1978).
Broome, S. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 75, 2746–2749 (1978).
Livingston, D. M. Meth. Enzym. 34, 723–731 (1974).
Hunter, W. M. & Greenwood, F. C. Biochem. J. 91, 43–46 (1964).
Rodriguez, R. L., West, R. W., Heyneker, H. L., Bolivar, F. & Boyer, H. W. Nucleic Acids Res. 6, 3267–3287 (1979).
Widera, G., Gautier, F., Lindenmaier, W. & Collins, J. Molec. gen. Genet. 163, 301–305 (1978).
Boyer, H. W. et al. in Recombinant Molecules: Impact on Science and Society (eds Beers, R. F. & Bassett, E. G.) 9–20 (Raven, New York, 1971).
Genetic Engineering: New Guidelines for U.K. Nature 276, 104–108 (1978).
Platt, T. in The Operon (eds Miller,, J. H. & Reznikoff, W. S.) 263–302 (Cold Spring Harbor Laboratory, New York, 1978).
Squires, C. L., Lee, F. D. & Yanofsky, C. J. molec. Biol. 92, 93–111 (1975).
Waterfield, M. D., Espelie, K., Elder, K. & Skehel, J. J. Br. med. Bull. 35, 57–63 (1979).
Adhya, S. & Gottesman, M. A. Rev. Biochem. 47, 967–996 (1978).
Hallewell, R. A. & Emtage, J. S. Gene (in the press).
Mercereau-Puijalon, O. et al. Nature 275, 505–510 (1978).
Fraser, T. H. & Bruce, B. J. Proc. natn. Acad. Sci. U.S.A. 75, 5936–5940 (1978).
Kessler, S. W. J. Immun. 115, 1617–1624 (1975).
Laemmli, U. K. Nature 227, 680–685 (1970).
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Emtage, J., Tacon, W., Catlin, G. et al. Influenza antigenic determinants are expressed from haemagglutinin genes cloned in Escherichia coli. Nature 283, 171–174 (1980). https://doi.org/10.1038/283171a0
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DOI: https://doi.org/10.1038/283171a0
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