Zinc finger nuclease

Definition

Zinc finger nucleases (ZFNs) are synthetic proteins used for gene targeting. They consist of a DNA-cutting endonculease domain fused to zinc finger domains engineered to bind a specific DNA sequence. ZFNs are used to introduce insertions or deletions at cut sites in the genomes of living cells.

Latest Research and Reviews

  • Protocols |

    CRISPR–Cas9 has truly democratized genome editing. In this Perspective, Jin-Soo Kim discusses CRISPR–Cas9 genome editing in the context of earlier innovations using meganucleases, ZFNs and TALENs, which paved the way for the ongoing CRISPR–Cas revolution.

    • Jin-Soo Kim
    Nature Protocols 11, 1573–1578
  • Reviews |

    Programmable nucleases — including ZFNs, TALENs and RGENs derived from the prokaryotic CRISPR–Cas system — enable targeted high-precision genome engineering. This Review summarizes current knowledge of nuclease-specific features, individual pros and cons, and approaches to improve the efficiency of these enzymes to help researchers to choose the most appropriate tool for various genome editing experiments and applications.

    • Hyongbum Kim
    •  & Jin-Soo Kim
  • Research |

    Due to an unexpected cell-penetrating property, zinc-finger nucleases (ZFNs) can be delivered to several mammalian cell types as proteins. Dose-dependent disruption of an endogenous gene was achieved with reduced activity at known off-target sites.

    • Thomas Gaj
    • , Jing Guo
    • , Yoshio Kato
    • , Shannon J Sirk
    •  & Carlos F Barbas III
    Nature Methods 9, 805–807
  • Research |

    Conditional genetic knockout is achieved in the rat by using zinc-finger nucleases to place loxP sites at specific genomic locations and introducing Cre recombinase under the control of a native promoter.

    • Andrew J Brown
    • , Daniel A Fisher
    • , Evguenia Kouranova
    • , Aaron McCoy
    • , Kevin Forbes
    • , Yumei Wu
    • , Rachel Henry
    • , Diana Ji
    • , Andre Chambers
    • , Joe Warren
    • , Weiguo Shu
    • , Edward J Weinstein
    •  & Xiaoxia Cui
    Nature Methods 10, 638–640
  • Research |

    This paper reports genetic manipulation of the malaria parasite Plasmodium falciparum with zinc-finger nucleases. It demonstrates gene disruption as well as replacement and site-specific editing of both an integrated reporter and an endogenous gene.

    • Judith Straimer
    • , Marcus C S Lee
    • , Andrew H Lee
    • , Bryan Zeitler
    • , April E Williams
    • , Jocelynn R Pearl
    • , Lei Zhang
    • , Edward J Rebar
    • , Philip D Gregory
    • , Manuel Llinás
    • , Fyodor D Urnov
    •  & David A Fidock
    Nature Methods 9, 993–998
  • Research |

    • Hojun Li
    • , Virginia Haurigot
    • , Yannick Doyon
    • , Tianjian Li
    • , Sunnie Y. Wong
    • , Anand S. Bhagwat
    • , Nirav Malani
    • , Xavier M. Anguela
    • , Rajiv Sharma
    • , Lacramiora Ivanciu
    • , Samuel L. Murphy
    • , Jonathan D. Finn
    • , Fayaz R. Khazi
    • , Shangzhen Zhou
    • , David E. Paschon
    • , Edward J. Rebar
    • , Frederic D. Bushman
    • , Philip D. Gregory
    • , Michael C. Holmes
    •  & Katherine A. High
    Nature 475, 217–221

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