Western blot

The western blot is a method for detecting the presence and size of specific proteins. Proteins are first separated according to size or other physical properties by gel electrophoresis, then transferred to a membrane (blotted) before a detection step that uses labelled antibody to recognize the protein of interest.

Latest Research and Reviews

Research | 11 September 2017 | open

Molecular details of secretory phospholipase A₂ from flax (Linum usitatissimum L.) provide insight into its structure and function
- Payal Gupta
- & Prasanta K. Dash
Scientific Reports 7, 11080
Research | 26 September 2016 | open

Protein characterization of intracellular target-sorted, formalin-fixed cell subpopulations
- Jessica S. Sadick
- , Molly E. Boutin
- , Diane Hoffman-Kim
- & Eric M. Darling
Scientific Reports 6, 33999
Protocols | 28 July 2016

Single cell–resolution western blotting

Single-cell-resolution western blotting concatenates an electrophoretic separation to downstream immunoprobing of unfixed mammalian cells to detect proteins, isoforms, and interactions that are not discernable by immunoreactivity alone.
- Chi-Chih Kang
- , Kevin A Yamauchi
- , Julea Vlassakis
- , Elly Sinkala
- , Todd A Duncombe
- & Amy E Herr
Nature Protocols 11, 1508-1530
Research | 18 July 2016

DNMT3A and TET2 compete and cooperate to repress lineage-specific transcription factors in hematopoietic stem cells

Margaret Goodell, Wei Li and colleagues use double-knockout mice for Dnmt3a and Tet2 to model leukemia development. Through epigenetic and transcriptional analyses, they show that loss of DNMT3A and TET2 upregulates lineage-specific transcription factors such as KLF1 in hematopoietic stem cells and accelerates malignancy.
- Xiaotian Zhang
- , Jianzhong Su
- , Mira Jeong
- , Myunggon Ko
- , Yun Huang
- , Hyun Jung Park
- , Anna Guzman
- , Yong Lei
- , Yung-Hsin Huang
- , Anjana Rao
- , Wei Li
- & Margaret A Goodell
Nature Genetics 48, 1014-1023
Protocols | 03 March 2011

Investigating ADAMTS-mediated aggrecanolysis in mouse cartilage
- Heather Stanton
- , Suzanne B Golub
- , Fraser M Rogerson
- , Karena Last
- , Christopher B Little
- & Amanda J Fosang
Nature Protocols 6, 388-404
Research | 24 January 2010

Systems analysis of EGF receptor signaling dynamics with microwestern arrays

Microwestern arrays combine the advantages of scalability of reverse phase protein arrays and the information content of western blotting for analyzing protein abundance and modification state with high sensitivity and throughput. The method is demonstrated for analyzing phosphorylation state changes in the EGF receptor signaling network using Bayesian network modeling.
- Mark F Ciaccio
- , Joel P Wagner
- , Chih-Pin Chuu
- , Douglas A Lauffenburger
- & Richard B Jones
Nature Methods 7, 148-155

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News and Comment

Research Highlights | 30 November 2018

Enhanced antibody validation
- Allison Doerr
Nature Methods 15, 1001
Correspondence | 28 February 2017

Should we ignore western blots when selecting antibodies for other applications?
- Fridtjof Lund-Johansen
- & Michael D Browning
Nature Methods 14, 215
Correspondence | 28 February 2017

Response to: Should we ignore western blots when selecting antibodies for other applications?
- Mathias Uhlen
Nature Methods 14, 215-216
Research Highlights | 23 June 2014

Technique: Single-cell western blotting
- Eytan Zlotorynski
Nature Reviews Molecular Cell Biology 15, 429
News | 20 December 2012

Scalable, automated microfluidic western blotting

A scalable, automated microfluidics system could help improve the efficiency of western blots.

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Western blot

Latest Research and Reviews

Molecular details of secretory phospholipase A₂ from flax (Linum usitatissimum L.) provide insight into its structure and function

Protein characterization of intracellular target-sorted, formalin-fixed cell subpopulations

Single cell–resolution western blotting

DNMT3A and TET2 compete and cooperate to repress lineage-specific transcription factors in hematopoietic stem cells

Investigating ADAMTS-mediated aggrecanolysis in mouse cartilage

Systems analysis of EGF receptor signaling dynamics with microwestern arrays

News and Comment

Enhanced antibody validation

Should we ignore western blots when selecting antibodies for other applications?

Response to: Should we ignore western blots when selecting antibodies for other applications?

Technique: Single-cell western blotting

Scalable, automated microfluidic western blotting

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Western blot

Latest Research and Reviews

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