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| Open AccessGenetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin
Centromeres are a self-propagating chromatin structure that feature nucleosomes containing histone H3 variant CENP-A. Here, the authors screen for factors that play a role in CENP-A chromatin maintenance, finding that SUMO-protease SENP6 controls inheritance of chromatin bound CENP-A and is required for the maintenance of the centromere and kinetochore complex.
- Sreyoshi Mitra
- , Dani L. Bodor
- & Lars E. T. Jansen
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Article
| Open AccessSUMO-mediated regulation of NLRP3 modulates inflammasome activity
The NLRP3 inflammasome is an important component of inflammatory responses, but how it is negatively regulated is still unclear. Here the authors show that post-translational modification of NLRP3 by sumoylation suppresses inflammasome activity, and that desumoylation of NLRP3 by the SENP6 and SENP7 proteases promotes NLRP3 activation.
- Rachael Barry
- , Sidonie Wicky John
- & Pascal Meier
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| Open AccessUncovering the SUMOylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification
Ubiquitylation and SUMOylation are two important related post-translational modifications. Here the authors present an approach for the simultaneous identification and quantification of protein-wide SUMO and ubiquitin sites from a single sample, uncovering widespread crosstalk between the two modifications.
- Frédéric Lamoliatte
- , Francis P. McManus
- & Pierre Thibault
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Article
| Open AccessSeveral posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission
Gephyrin is a cytoplasmic scaffolding protein that selectively forms postsynaptic scaffolds at GABAergic and glycinergic synapses. Here the authors characterize regulatory mechanisms determining gephyrin scaffolding and GABAA receptor synaptic transmission that involve acetylation, SUMOylation and phosphorylation.
- Himanish Ghosh
- , Luca Auguadri
- & Shiva K. Tyagarajan