RNA sequencing

Definition

RNA sequencing refers to techniques used to determine the sequence of RNA molecules. It includes high-throughput shotgun sequencing of cDNA molecules obtained by reverse transcription from RNA, and next-generation sequencing technologies to sequence the RNA molecules within a biological sample in an effort to determine the primary sequence and relative abundance of each RNA molecule.

Latest Research and Reviews

  • Research |

    One hundred and ten Zika virus genomes from ten countries and territories involved in the Zika virus epidemic reveal rapid expansion of the epidemic within Brazil and multiple introductions to other regions.

    • Hayden C. Metsky
    • , Christian B. Matranga
    • , Shirlee Wohl
    • , Stephen F. Schaffner
    • , Catherine A. Freije
    • , Sarah M. Winnicki
    • , Kendra West
    • , James Qu
    • , Mary Lynn Baniecki
    • , Adrianne Gladden-Young
    • , Aaron E. Lin
    • , Christopher H. Tomkins-Tinch
    • , Simon H. Ye
    • , Daniel J. Park
    • , Cynthia Y. Luo
    • , Kayla G. Barnes
    • , Rickey R. Shah
    • , Bridget Chak
    • , Giselle Barbosa-Lima
    • , Edson Delatorre
    • , Yasmine R. Vieira
    • , Lauren M. Paul
    • , Amanda L. Tan
    • , Carolyn M. Barcellona
    • , Mario C. Porcelli
    • , Chalmers Vasquez
    • , Andrew C. Cannons
    • , Marshall R. Cone
    • , Kelly N. Hogan
    • , Edgar W. Kopp
    • , Joshua J. Anzinger
    • , Kimberly F. Garcia
    • , Leda A. Parham
    • , Rosa M. Gélvez Ramírez
    • , Maria C. Miranda Montoya
    • , Diana P. Rojas
    • , Catherine M. Brown
    • , Scott Hennigan
    • , Brandon Sabina
    • , Sarah Scotland
    • , Karthik Gangavarapu
    • , Nathan D. Grubaugh
    • , Glenn Oliveira
    • , Refugio Robles-Sikisaka
    • , Andrew Rambaut
    • , Lee Gehrke
    • , Sandra Smole
    • , M. Elizabeth Halloran
    • , Luis Villar
    • , Salim Mattar
    • , Ivette Lorenzana
    • , Jose Cerbino-Neto
    • , Clarissa Valim
    • , Wim Degrave
    • , Patricia T. Bozza
    • , Andreas Gnirke
    • , Kristian G. Andersen
    • , Sharon Isern
    • , Scott F. Michael
    • , Fernando A. Bozza
    • , Thiago M. L. Souza
    • , Irene Bosch
    • , Nathan L. Yozwiak
    • , Bronwyn L. MacInnis
    •  & Pardis C. Sabeti
  • Research | | open

    Human T-cell leukaemia virus type-1 and bovine leukaemia virus infect T and B lymphocytes and lead to aggressive leukaemia. Here, the authors show these proviruses integrate near cancer drivers perturbing transcription termination or antisense RNA-dependent interaction, suggesting post-transcriptional mechanisms in some cases.

    • Nicolas Rosewick
    • , Keith Durkin
    • , Maria Artesi
    • , Ambroise Marçais
    • , Vincent Hahaut
    • , Philip Griebel
    • , Natasa Arsic
    • , Véronique Avettand-Fenoel
    • , Arsène Burny
    • , Carole Charlier
    • , Olivier Hermine
    • , Michel Georges
    •  & Anne Van den Broeke
  • Reviews |

    Next-generation sequencing technologies have enabled the comprehensive characterization of human and mouse genomes, including at the transcriptional level. This article reviews the degree of conservation of human and mouse transcriptomes, along with the challenges of identifying when the mouse is a suitable model of human physiology.

    • Alessandra Breschi
    • , Thomas R. Gingeras
    •  & Roderic Guigó
  • Research |

    An effective and high-throughput technology spatially profiles the whole transcriptome of intact plant tissues from Arabidopsis thaliana, Populus tremula and Picea abies. It is also suitable for other species that are less genetically tractable.

    • Stefania Giacomello
    • , Fredrik Salmén
    • , Barbara K. Terebieniec
    • , Sanja Vickovic
    • , José Fernandez Navarro
    • , Andrey Alexeyenko
    • , Johan Reimegård
    • , Lauren S. McKee
    • , Chanaka Mannapperuma
    • , Vincent Bulone
    • , Patrik L. Ståhl
    • , Jens F. Sundström
    • , Nathaniel R. Street
    •  & Joakim Lundeberg
    Nature Plants 3, 17061

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