RNA-binding proteins

RNA-binding proteins are proteins that bind to ribonucleic acid (RNA) molecules, are generally found in the cytoplasm and nucleus, and are important in forming ribonucleoproteins (RNPs). One type of RNP, hnRNPs (heteronuclear proteins), are important in splicing of mRNA (messenger RNA), polyadenylation, stabilization, localization and translation.

Latest Research and Reviews

  • Research | | open

    Comprehensive characterisation of RNA-protein interactions requires different levels of resolution. Here, the authors present an integrated mass spectrometry-based approach that allows them to define the Drosophila RNA-protein interactome from the level of multisubunit complexes down to the RNA-binding amino acid.

    • Amol Panhale
    • , Florian M. Richter
    • , Fidel Ramírez
    • , Maria Shvedunova
    • , Thomas Manke
    • , Gerhard Mittler
    •  & Asifa Akhtar
  • Reviews |

    Enabled by genome-wide profiling approaches, there is growing appreciation for the prevalence and functional importance of various types of chromatin-associated RNAs. As Li and Fu describe in this Review, these RNAs can either be retained in cis at their site of transcription or recruited in cis to other loci, and they have diverse roles in gene regulation, genome organization, nuclear body formation and phase-separation events.

    • Xiao Li
    •  & Xiang-Dong Fu
  • Research | | open

    Low complexity (LC) domains can drive the formation of both amyloid fibrils and protein droplets. Here, the authors identify reversible amyloid cores from the LC of hnRNPA1, based on which they elucidate the structural basis of reversible fibrillation and its interplay with hnRNPA1 droplet formation.

    • Xinrui Gui
    • , Feng Luo
    • , Yichen Li
    • , Heng Zhou
    • , Zhenheng Qin
    • , Zhenying Liu
    • , Jinge Gu
    • , Muyun Xie
    • , Kun Zhao
    • , Bin Dai
    • , Woo Shik Shin
    • , Jianhua He
    • , Lin He
    • , Lin Jiang
    • , Minglei Zhao
    • , Bo Sun
    • , Xueming Li
    • , Cong Liu
    •  & Dan Li
  • Research | | open

    The Staufen family of RNA-binding proteins are conserved microtubule dependent mRNA transporter factors. Here the authors use biochemical and functional approaches to characterize the RNA-binding properties of mouse Staufen 2 and study the mRNA binding capacity of its two domains dsRBDs 1 and 2.

    • Simone Heber
    • , Imre Gáspár
    • , Jan-Niklas Tants
    • , Johannes Günther
    • , Sandra M. Fernandez Moya
    • , Robert Janowski
    • , Anne Ephrussi
    • , Michael Sattler
    •  & Dierk Niessing
  • Research |

    Doudna and colleagues determine the mechanisms used by type V anti-CRISPR proteins. AcrVA1 is a multiple-turnover inhibitor that triggers cleavage of the Cas12a-bound guide RNA, while AcrVA4 and AcrVA5 inhibit recognition of dsDNA.

    • Gavin J. Knott
    • , Brittney W. Thornton
    • , Marco J. Lobba
    • , Jun-Jie Liu
    • , Basem Al-Shayeb
    • , Kyle E. Watters
    •  & Jennifer A. Doudna

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