Replisome articles from across Nature Portfolio

Poly(ADP-ribose) polymerase 1 (PARP1) functions together with TIMELESS and TIPIN to protect the replisome in early S phase from transcription–replication conflicts, and inhibiting PARP1 enzymatic activity may suffice for treatment efficacy in homologous recombination-deficient settings.

We study the interplay between cohesin and replication by reconstituting a functional replisome using purified proteins, showing how cohesin initially responds to replication and providing a molecular model for the establishment of sister chromatid cohesion.

Coordinating the activities of replicative helicase and DNA polymerase(s) is crucial for DNA replication. Here, the authors show that DNA translocation by CMG is synchronized with the activities of Polε in the leading-strand replisome.

Here the authors report how cohesin loader Scc2 is recruited to chromatin during replication by the processivity-promoting factor PCNA to support de novo cohesin loading onto replicated sister DNA and ensure sister chromatid cohesion.

The mechanism of replication restart initiation by the bacterial DNA replication restart proteins PriA and PriB is resolved, revealing a switch-like restructuring of PriA triggered by replication fork binding that mediates PriA/PriB complex assembly.

Here the authors present the structure of Replication Protein A (RPA) in Archaea. The RPA structure from P. abyssi has been determined in presence and absence of DNA, providing insights into the evolution of this replication factor in eukaryotes