Ciric et al. describe a protocol for the removal of motion artifacts from functional MRI data. They introduce a software package that implements common denoising protocols and provides tools for assessing the efficacy of denoising.

DNA barcode swapping results in mislabelling of sequencing reads between multiplexed samples. Here, the authors investigate the severity and consequences of barcode swapping for single-cell RNA-seq data, and develop a computational method to exclude swapped reads.

This protocol describes a set of assays for characterization of the quality and quantity of mammalian collagen type I from a diverse range of biological samples.

Technical errors can hamper the interpretation of next-generation sequencing (NGS) data, which poses a major challenge for the clinical application of this technology. This Review discusses how reference standards circumvent this issue by calibrating NGS measurements and evaluating diagnostic performance of NGS-based genetic tests.