Protein sequencing

Definition

Protein sequencing refers to methods for determining the amino acid sequence of proteins (or peptides) and analysis of the sequence, for example to infer protein conformation. Techniques include mass spectrometry and the Edman degradation reaction as well as prediction of the protein sequence from the encoding DNA or mRNA sequence.

Latest Research and Reviews

  • Research |

    A draft map of the human proteome is presented here, accounting for over 80% of the annotated protein-coding genes in humans; some novel protein-coding regions, including translated pseudogenes, non-coding RNAs and upstream open reading frames, are identified.

    • Min-Sik Kim
    • , Sneha M. Pinto
    • , Derese Getnet
    • , Raja Sekhar Nirujogi
    • , Srikanth S. Manda
    • , Raghothama Chaerkady
    • , Anil K. Madugundu
    • , Dhanashree S. Kelkar
    • , Ruth Isserlin
    • , Shobhit Jain
    • , Joji K. Thomas
    • , Babylakshmi Muthusamy
    • , Pamela Leal-Rojas
    • , Praveen Kumar
    • , Nandini A. Sahasrabuddhe
    • , Lavanya Balakrishnan
    • , Jayshree Advani
    • , Bijesh George
    • , Santosh Renuse
    • , Lakshmi Dhevi N. Selvan
    • , Arun H. Patil
    • , Vishalakshi Nanjappa
    • , Aneesha Radhakrishnan
    • , Samarjeet Prasad
    • , Tejaswini Subbannayya
    • , Rajesh Raju
    • , Manish Kumar
    • , Sreelakshmi K. Sreenivasamurthy
    • , Arivusudar Marimuthu
    • , Gajanan J. Sathe
    • , Sandip Chavan
    • , Keshava K. Datta
    • , Yashwanth Subbannayya
    • , Apeksha Sahu
    • , Soujanya D. Yelamanchi
    • , Savita Jayaram
    • , Pavithra Rajagopalan
    • , Jyoti Sharma
    • , Krishna R. Murthy
    • , Nazia Syed
    • , Renu Goel
    • , Aafaque A. Khan
    • , Sartaj Ahmad
    • , Gourav Dey
    • , Keshav Mudgal
    • , Aditi Chatterjee
    • , Tai-Chung Huang
    • , Jun Zhong
    • , Xinyan Wu
    • , Patrick G. Shaw
    • , Donald Freed
    • , Muhammad S. Zahari
    • , Kanchan K. Mukherjee
    • , Subramanian Shankar
    • , Anita Mahadevan
    • , Henry Lam
    • , Christopher J. Mitchell
    • , Susarla Krishna Shankar
    • , Parthasarathy Satishchandra
    • , John T. Schroeder
    • , Ravi Sirdeshmukh
    • , Anirban Maitra
    • , Steven D. Leach
    • , Charles G. Drake
    • , Marc K. Halushka
    • , T. S. Keshava Prasad
    • , Ralph H. Hruban
    • , Candace L. Kerr
    • , Gary D. Bader
    • , Christine A. Iacobuzio-Donahue
    • , Harsha Gowda
    •  & Akhilesh Pandey
    Nature 509, 575–581
  • Research |

    The combination of protein display, moderate selection for protein activity and high-throughput DNA sequencing can be applied to hundreds of thousands of protein variants in parallel, enabling the derivation of sequence-function relationships.

    • Douglas M Fowler
    • , Carlos L Araya
    • , Sarel J Fleishman
    • , Elizabeth H Kellogg
    • , Jason J Stephany
    • , David Baker
    •  & Stanley Fields
    Nature Methods 7, 741–746
  • Research |

    The complexity and dynamic range of mammalian proteomes has stymied comprehensive protein quantification for the past twenty years. Zhou et al. develop DEEP SEQ mass spectrometry and use it to quantify a murine stem cell proteome to a depth equivalent to RNA-seq-based ribosome profiling.

    • Feng Zhou
    • , Yu Lu
    • , Scott B. Ficarro
    • , Guillaume Adelmant
    • , Wenyu Jiang
    • , C. John Luckey
    •  & Jarrod A. Marto

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