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Next-generation sequencing refers to non-Sanger-based high-throughput DNA sequencing technologies. Millions or billions of DNA strands can be sequenced in parallel, yielding substantially more throughput and minimizing the need for the fragment-cloning methods that are often used in Sanger sequencing of genomes.
A diverse range of viruses of the yak gut in Qinghai-Tibet Plateau is presented, showing significant sequence identity across regions. Also regional differences in gene function and significant virus-bacterium symbiotic networks were uncovered.
In this Tools of the Trade article, Jeroen van den Berg (van Oudenaarden lab) presents a new method based on pulse-labelling of nascent DNA to study the dynamics of DNA replication in single cells.
Exome-sequencing analysis in a Chinese cohort of 1,578 children with cerebral palsy established a genetic etiology in 387 of the patients (24.5%). Children with cerebral palsy for whom perinatal asphyxia had been recorded at birth were found to be more likely to carry pathogenic or potentially pathogenic variants.
The discovery of antibodies that bind with high affinity to clinically relevant antigens can be sped up by leveraging next-generation sequencing to screen hundreds of millions of antibody–antigen interactions.
A trimodal single-cell assay reveals a previously unknown T cell subset and cellular state differences between children and older adults that might contribute to age-specific immunity.