Membrane proteins

Membrane proteins are proteins found in cell membranes, either at the surface or on intracellular organelles. Integral membrane proteins such as receptors and ion channels span the membrane. Peripheral membrane proteins, for example some signalling proteins, are tethered by an anchor, via a fatty acid, prenyl group, glycophosphatidylinositol (GPI) or a hydrophobic protein patch.

Featured

Latest Research and Reviews

  • Research | | open

    The leading cause of cystic fibrosis is the deletion of phenylalanine 508 (F508del) in the first nucleotide-binding domain (NBD1) of the cystic fibrosis transmembrane conductance regulator (CFTR). Here authors we develop nanobodies targeting NBD1 of human CFTR and demonstrate their ability to stabilize both isolated NBD1 and full-length protein.

    • Maud Sigoillot
    • , Marie Overtus
    • , Magdalena Grodecka
    • , Daniel Scholl
    • , Abel Garcia-Pino
    • , Toon Laeremans
    • , Lihua He
    • , Els Pardon
    • , Ellen Hildebrandt
    • , Ina Urbatsch
    • , Jan Steyaert
    • , John R. Riordan
    •  & Cedric Govaerts
  • Research | | open

    The transport of L-lactate across plasma membranes is catalyzed by proton-driven monocarboxylate transporters (MCTs) of the SLC16 solute carrier family. Here, the authors present the crystal structures of a bacterial SLC16 homologue with the bound substrate L-lactate and ligand thiosalicylate both in an outward-open conformation and discuss the L-lactate transport mechanism.

    • Patrick D. Bosshart
    • , David Kalbermatter
    • , Sara Bonetti
    •  & Dimitrios Fotiadis
  • Research | | open

    Type VI secretion systems (T6SS) are important for bacterial interaction, competition and virulence, but the abundance and assembly of their components is still not well understood. Here, the authors apply targeted proteomics to measure the abundance of T6SS components across different species and conditions.

    • Lin Lin
    • , Emmanuelle Lezan
    • , Alexander Schmidt
    •  & Marek Basler
  • Research |

    Cryo-EM structure of the LAT1–CD98hc heterodimer in complex with two antibodies offers insights into the assembly and function of LAT1–CD98hc, and reveals the epitopes targeted by the potentially therapeutic antibodies with an antitumor activity.

    • Yongchan Lee
    • , Pattama Wiriyasermkul
    • , Chunhuan Jin
    • , Lili Quan
    • , Ryuichi Ohgaki
    • , Suguru Okuda
    • , Tsukasa Kusakizako
    • , Tomohiro Nishizawa
    • , Kazumasa Oda
    • , Ryuichiro Ishitani
    • , Takeshi Yokoyama
    • , Takanori Nakane
    • , Mikako Shirouzu
    • , Hitoshi Endou
    • , Shushi Nagamori
    • , Yoshikatsu Kanai
    •  & Osamu Nureki
  • Research | | open

    Hedgehog (Hh) controls embryonic development via interaction with its receptor Patched (Ptch). Here the authors report the cryo-EM structure of tetrameric Ptch1 in complex with the palmitoylated N-terminal signaling domain of human Sonic hedgehog (ShhNp) at a 4:2 stoichiometric ratio.

    • Hongwu Qian
    • , Pingping Cao
    • , Miaohui Hu
    • , Shuai Gao
    • , Nieng Yan
    •  & Xin Gong

News and Comment

  • News and Views |

    The σ1 receptor, an endoplasmic reticulum–resident transmembrane protein, modulates many physiological and pathological processes and binds multiple drugs, but is nonetheless poorly understood. In a recent issue, Kruse and colleagues illustrate structural differences between agonist- and antagonist-bound receptor and propose that agonist binding may impair oligomerization, making a major step in understanding σ1 function. They also use a combination of kinetic and molecular dynamic modeling to explain how ligands access the binding pocket.

    • Felix J. Kim
    •  & Gavril W. Pasternak
  • News and Views |

    The polycystin complex structure has been solved at near-atomic resolution. Its surprising architecture provides new insights into the transient receptor potential (TRP) family of cation channels and the pathogenesis of autosomal dominant polycystic kidney disease. This discovery should have a transformative impact on the development of treatment strategies to cure the disease.

    • Paul A. Welling