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Contextual dependencies expand the re-usability of genetic inverters
Genetic circuits can be engineered to generate predefined outcomes, however host context is a crucial factor in performance. Here the authors characterise twenty NOT gates in seven different bacteria to understand and predict interoperability and portability across hosts.
- Huseyin Tas
- , Lewis Grozinger
- & Ángel Goñi-Moreno
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Article
| Open Access
High-performance chemical- and light-inducible recombinases in mammalian cells and mice
The availability of high performance recombinases with low basal activity and high dynamic range is limited. Here the authors present a library of over 20 orthogonal split recombinases that can be induced by small molecules, light and temperature in vivo.
- Benjamin H. Weinberg
- , Jang Hwan Cho
- & Wilson W. Wong
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Article
| Open Access
Switching the activity of Cas12a using guide RNA strand displacement circuits
Cas12a is a useful alternative to Cas9 for genome editing and regulation. Here the authors design strand displacement gRNAs that can add functionality to Cas12a by acting as multi-input logic gates.
- Lukas Oesinghaus
- & Friedrich C. Simmel
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Article
| Open Access
Engineering orthogonal dual transcription factors for multi-input synthetic promoters
Genetic circuits usually employ the same set of transcription factors which can act via repression or activation of the target promoter. Here the authors present dual activator-repressor switches, designed via directed evolution, for orthogonal logic gates and multi-input circuit architectures.
- Andreas K. Brödel
- , Alfonso Jaramillo
- & Mark Isalan
A computer-guided design tool to increase the efficiency of cellular conversions