Featured
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News & Views |
Signal transduction with a swing
The continuous monitoring of proteins is a current challenge in medical diagnostics. A new electrochemical approach aiming to address this has been described. The method uses antibodies as a recognition element to achieve the real-time measurement of proteins in saliva in the mouth.
- Kevin J. Cash
- & Kevin W. Plaxco
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Article |
Reagentless biomolecular analysis using a molecular pendulum
A reagentless method for detecting analytes based on the motion of an inverted molecular pendulum has now been developed. The sensor is capable of detecting important physiological markers of stress, allergy, cardiovascular health, inflammation and cancer and works in blood, saliva, urine, tears and sweat. The sensor is also capable of collecting data in living animals.
- Jagotamoy Das
- , Surath Gomis
- & Shana O. Kelley
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Meeting Report |
No small matter
The confined geometry of nanopores enables a wealth of chemistry and analysis to be conducted at the single-molecule scale. Yi-Lun Ying, Aleksandar P. Ivanov and Vincent Tabard-Cossa report on recent developments discussed at the 2020 Nanopore Electrochemistry Meeting.
- Yi-Lun Ying
- , Aleksandar P. Ivanov
- & Vincent Tabard-Cossa
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News & Views |
Unique enzymatic repertoire reveals the tumour
The tumour microenvironment has a specific enzymatic fingerprint, which provides opportunities for cancer therapy. Now, two studies show how this unique chemical environment can be used to produce reporter molecules or nanoclusters within the tumour that can subsequently be identified in urine or breath, enabling cancer detection and monitoring.
- Alexander N. Zelikin
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Article |
Designer DNA architecture offers precise and multivalent spatial pattern-recognition for viral sensing and inhibition
DNA is capable of self-assembling into a wide range of user-defined structures and so can be used as a scaffold to arrange binding motifs with nanometre precision. Now, DNA has been used to accurately display aptamers that fit the repeated epitope pattern of a dengue viral antigen to produce a nanostructure that can be a potent viral inhibitor or a fluorescent sensor.
- Paul S. Kwon
- , Shaokang Ren
- & Xing Wang
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Article |
A molecular multi-gene classifier for disease diagnostics
Gene expression profiling remains cost-prohibitive and challenging to implement in a clinical setting. Now, a molecular computation strategy for classifying complex gene expression signatures has been developed. Classification occurs through a series of molecular interactions between RNA inputs and engineered DNA probes designed to implement a relevant linear classification model.
- Randolph Lopez
- , Ruofan Wang
- & Georg Seelig
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Article |
A monodisperse transmembrane α-helical peptide barrel
The assembly of transmembrane barrels formed from short synthetic peptides has not been previously demonstrated. Now, a transmembrane pore has been fabricated via the self-assembly of peptides. The 35-amino-acid α-helical peptides are based on the C-terminal D4 domain of the Escherichia coli polysaccharide transporter Wza.
- Kozhinjampara R. Mahendran
- , Ai Niitsu
- & Hagan Bayley
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News & Views |
Clamping down on cancer detection
An electrochemical clamp assay that enables the rapid and sensitive detection of nucleic acids containing single base mutations has now been developed. It has been shown to differentiate between cancer patient samples featuring a specific mutation, and controls from healthy donors or other cancer patients, all directly in unprocessed serum.
- Irina A. Gorodetskaya
- & Alon A. Gorodetsky
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Article |
Through-space transfer of chiral information mediated by a plasmonic nanomaterial
Surface-enhanced resonant Raman optical activity (SERROA) reveals the through-space transfer of chirality from biomolecules to achiral benzotriazole dye-conjugated nanotags. The chiroptical responses generated by the stereoisomers of ribose and tryptophan establish this as the basis for a stereoselective nanosensor platform.
- Saeideh Ostovar pour
- , Louise Rocks
- & Ewan W. Blanch
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Article |
Conditionally fluorescent molecular probes for detecting single base changes in double-stranded DNA
A molecular probe has been designed that distinguishes double-stranded DNA with single base-pair specificity. In this approach, two destabilizing bubbles, in which the base pairs are mismatched, are generated for each point mutation in the target DNA.
- Sherry Xi Chen
- , David Yu Zhang
- & Georg Seelig