Bacterial structural biology

Bacterial structural biology is a scientific discipline that concerns the structure of bacterial macromolecules, especially proteins and nucleic acids. Such studies are used to understand the function and properties of components of the bacterial cell.

Latest Research and Reviews

  • Research | | open

    Multidrug efflux pumps actively expel a wide range of toxic substrates from bacteria and play a major role in drug resistance. Here authors show the in situ structure of the efflux pump AcrAB-TolC obtained by electron cryo-tomography and subtomogram averaging.

    • Xiaodong Shi
    • , Muyuan Chen
    • , Zhili Yu
    • , James M. Bell
    • , Hans Wang
    • , Isaac Forrester
    • , Heather Villarreal
    • , Joanita Jakana
    • , Dijun Du
    • , Ben F. Luisi
    • , Steven J. Ludtke
    •  & Zhao Wang
  • Reviews |

    Lipopolysaccharide is a key component of the Gram-negative cell envelope and functions, for example, as a permeability barrier or determinant of host immune responses. In this Review, Simpson and Trent guide us through lipopolysaccharide biogenesis and modifications and their functional and therapeutic implications.

    • Brent W. Simpson
    •  & M. Stephen Trent
  • Research | | open

    Autotransporter proteins are localised to the bacterial surface and promote colonisation of host epithelial surfaces. Here, the authors present the crystal structure of autotransporter UpaB and show evidence for distinct binding sites for glycosaminoglycans and host fibronectin.

    • Jason J. Paxman
    • , Alvin W. Lo
    • , Matthew J. Sullivan
    • , Santosh Panjikar
    • , Michael Kuiper
    • , Andrew E. Whitten
    • , Geqing Wang
    • , Chi-Hao Luan
    • , Danilo G. Moriel
    • , Lendl Tan
    • , Kate M. Peters
    • , Minh-Duy Phan
    • , Christine L. Gee
    • , Glen C. Ulett
    • , Mark A. Schembri
    •  & Begoña Heras
  • Research |

    A combination of electron cryotomography and immunofluorescence microscopy reveals the structure of the core transmembrane subcomplex of the Legionella defective in organelle trafficking (Dot)/intracellular multiplication (Icm) type IVB secretion system (T4BSS) and an early-stage assembly process by which T4BSS components are targeted to the bacterial poles by DotU and IcmF.

    • Debnath Ghosal
    • , Kwangcheol C. Jeong
    • , Yi-Wei Chang
    • , Jacob Gyore
    • , Lin Teng
    • , Adam Gardner
    • , Joseph P. Vogel
    •  & Grant J. Jensen
  • Reviews |

    Type IV pili (T4P) are dynamic filaments on the surface of many bacteria with essential roles in colonization and pathogenesis. In this Review, Craig, Forest and Maier examine the T4P architecture, present molecular models for T4P dynamics and highlight recent insights into T4P retraction.

    • Lisa Craig
    • , Katrina T. Forest
    •  & Berenike Maier
  • Research | | open

    Poly-ADP-ribosylation (PARylation) is a well-known regulatory event in eukaryotes but has not yet been observed in bacteria. Here, the authors solve the structure of a bacterial PAR-glycohydrolase and provide evidence for a prokaryotic PARylation machinery involved in the response to genotoxic stress.

    • Chao-Cheng Cho
    • , Chia-Yu Chien
    • , Yi-Chih Chiu
    • , Meng-Hsuan Lin
    •  & Chun-Hua Hsu

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