Abstract
At extravascular sites, monocyte/macrophage synthesis of complement protein is thought to be quantitatively important. Lipopolysaccharide(LPS) stimulates the production of C3 and other complement components.
In the present study human monocytes were isolated from peripheral blood and cultured for 1-5 days under serum-free conditions. GM-CSF or LPS was added both alone and in combination to selected monocyte populations on day 0. Supernatants were harvested on day 2 and day 5 and analysed for total C3 content in a sensitive enzyme-linked immunoassay.
Conclusion: GM-CSF is thought to increase resistance to infections, but the described effect may reduce complement opsonization and phagocytosis of pathogens in the tissues.
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Hoåsen, A., Abrahamsen, T. GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR (GM-CSF) SUPPRESSES BOTH SPONTANEOUS AND LIPOPOLYSACCHARIDE-STIMULATED BIOSYNTHESIS OF COMPLEMENT COMPONENT C3 IN CULTURED MONOCYTES. Pediatr Res 32, 623 (1992). https://doi.org/10.1203/00006450-199211000-00112
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DOI: https://doi.org/10.1203/00006450-199211000-00112